Annals of Laboratory Medicine

Korean Society for Laboratory Medicine (대한진단검사의학회)
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Multidrug Resistance Mechanisms of Carbapenem Resistant Klebsiella pneumoniae Strains Isolated in Chongqing, China

  • Yan, Jinrong (Department of Clinical Laboratory, The First Affiliated Hospital of Chongqing Medical University) ;
  • Pu, Shuli (Department of Clinical Laboratory, The First Affiliated Hospital of Chongqing Medical University) ;
  • Jia, Xiaojiong (Department of Clinical Laboratory, The First Affiliated Hospital of Chongqing Medical University) ;
  • Xu, Xiuyu (Department of Clinical Laboratory, The First Affiliated Hospital of Chongqing Medical University) ;
  • Yang, Shuangshuang (Department of Clinical Laboratory, The First Affiliated Hospital of Chongqing Medical University) ;
  • Shi, Jing (Department of Clinical Laboratory, The First Affiliated Hospital of Chongqing Medical University) ;
  • Sun, Shan (Department of Clinical Laboratory, The First Affiliated Hospital of Chongqing Medical University) ;
  • Zhang, Liping (Department of Clinical Laboratory, The First Affiliated Hospital of Chongqing Medical University)
  • Received : 2016.11.24
  • Accepted : 2017.05.22
  • Published : 2017.09.01
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Abstract

Background: Carbapenem-resistant Klebsiella pneumoniae (CRKP) is considered a serious global threat. However, little is known regarding the multidrug resistance (MDR) mechanisms of CRKP. This study investigated the phenotypes and MDR mechanisms of CRKP and identified their clonal characteristics. Methods: PCR and sequencing were utilized to identify antibiotic resistance determinants. Integron gene cassette arrays were determined by restriction fragment length polymorphism (RFLP) analysis. Multi-locus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) were used for epidemiological analysis. Plasmids were typed by using a PCR-based replicon typing and analyzed by conjugation and transformation assays. Results: Seventy-eight strains were identified as resistant to at least one carbapenem; these CRKP strains had a high prevalence rate (38.5%, 30/78) of carbapenemase producers. Additionally, most isolates harbored MDR genes, including Extended spectrum ${\beta}$-lactamases (ESBLs), AmpC, and quinolone and aminoglycoside resistance genes. Loss of porin genes was observed, and Class 1 integron was detected in 66.7% of the investigated isolates. PFGE and MLST results excluded the occurrence of clonal dissemination among these isolates. Conclusions: A high prevalence of NDM-1 genes encoding carbapenem resistance determinants was demonstrated among the K. pneumoniae isolates. Importantly, this is the first report of $bla_{NDM-1}$ carriage in a K. pneumoniae ST1383 clone in China and of a MDR CRKP isolate co-harboring $bla_{NDM-1}$, $bla_{KPC-2}$, $bla_{CTX-M}$, $bla_{SHV}$, acc(6')-Ib, rmtB, qnrB, and acc(6')-Ib-cr.

Keywords

Acknowledgement

Supported by : National Natural Science Foundation of China, Science Foundation of Yuzhong, district of Chongqing

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