Annals of Laboratory Medicine

Korean Society for Laboratory Medicine (대한진단검사의학회)
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Effect of Vitamin D Status on Von Willebrand Factor and ADAMTS13 in Diabetic Patients on Chronic Hemodialysis

  • Cohen-Hagai, Keren (Renal Physiology Laboratory, Department of Nephrology and Hypertension, Meir Medical Center) ;
  • Rashid, Gloria (Medical Laboratories, Meir Medical Center) ;
  • Einbinder, Yael (Renal Physiology Laboratory, Department of Nephrology and Hypertension, Meir Medical Center) ;
  • Ohana, Meital (Renal Physiology Laboratory, Department of Nephrology and Hypertension, Meir Medical Center) ;
  • Benchetrit, Sydney (Renal Physiology Laboratory, Department of Nephrology and Hypertension, Meir Medical Center) ;
  • Zitman-Gal, Tali (Renal Physiology Laboratory, Department of Nephrology and Hypertension, Meir Medical Center)
  • Received : 2016.07.11
  • Accepted : 2016.12.08
  • Published : 2017.03.01
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Abstract

Von Willebrand factor (vWF) is a glycoprotein with a crucial role in the formation of platelet thrombi, and ADAMTS13 is the main enzyme responsible for vWF cleavage. Both are important in the relationship between diabetic nephropathy, hypercoagulability, and cardiovascular disease. This study evaluated a potential relationship between vitamin D (vitD) levels, vWF, ADAMTS13 activity, and inflammation in diabetic patients on chronic hemodialysis (HD). Blood samples from 52 diabetic patients on chronic HD were obtained to determine vitD levels, vWF, and ADAMTS13 activity, and inflammatory markers. HD patients were grouped according to 25-hydroxyvitamin D [25(OH) VitD]<25 nmol/L (n=16) or >25 nmol/L (n=36). vWF antigen and vWF activity were elevated in both groups, with an average of $214.3{\pm}82.6%$ and $175.8{\pm}72.6%$, respectively. Average ADAMTS13 activity was within the normal range in both groups. Blood samples from the vitD <25 nmol/L group showed a positive correlation between c-reactive protein (CRP) and vWF levels (P =0.023; r=0.564; 95% confidence interval=0.095-0.828), with a negative correlation between HbA1c and 25(OH) VitD (P =0.015; r=-0.337; 95% confidence interval=-0.337-0.19). Diabetic patients on chronic HD had elevated vWF levels and activity with no significant change in ADAMTS13 activity. The correlation between CRP and vWF levels in the 25(OH) VitD<25 nmol/L group suggests inflammatory-related endothelial dysfunction in these patients.

Keywords

References

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