Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Incidence of Viral Diseases and Occurrence of Three Unreported Viruses in Yams in Korea

  • Lee, Joong-Hwan (Institute for Bioresources Research, Gyeongsangbuk-do Agricultural Research & Extension Services) ;
  • Park, Chung Youl (School of Applied Biosciences, Kyungpook National University) ;
  • Cho, Ha-Jeong (School of Applied Biosciences, Kyungpook National University) ;
  • Oh, Jonghee (School of Applied Biosciences, Kyungpook National University) ;
  • Kim, Bong-Sub (Functional Crop Resource Development Division, Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration) ;
  • Park, Eun Hey (Incheon International Airport Regional Office, Animal and Plant Quarantine Agency) ;
  • Son, Chang-Gi (Institute for Bioresources Research, Gyeongsangbuk-do Agricultural Research & Extension Services) ;
  • Lee, Su-Heon (School of Applied Biosciences, Kyungpook National University)
  • Received : 2016.11.10
  • Accepted : 2017.01.06
  • Published : 2017.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

During 2012 to 2014, a survey for the presence of viral diseases in yam plants was carried out in a field of the Institute for Bioresources Research in Gyeongsangbuk-do, Korea. A total of 88 leaf samples were collected and tested by reverse transcription polymerase chain reaction using specific primer sets. Eighty-one samples were positive for Broad bean wilt virus 2 (BBWV2), Chinese yam necrotic mosaic virus (ChYNMV), Cucumber mosaic virus (CMV), Japanese yam mosaic virus (JYMV), and Yam mild mosaic virus (YMMV), whereas Yam mosaic virus (YMV) was not detected. Additionally, seven samples were negative for all viruses. Several samples exhibited mixed (double and triple) infections. Three viruses (CMV, JYMV, and YMMV) were detected for the first time in yam plants in Korea. A BLAST search showed that three viruses shared nucleotide identities with CMV-Ca (98%), JYMV-O2 (91%), and YMMV-TG_NH_1 (86%). Thus, our findings confirmed that yam plants cultivated in Korea were infected with multiple viruses with three of these viruses reported for the first time in Korea.

Keywords

References

  1. Asiedu, R. and Sartie, A. 2010. Crops that feed the world 1. Yams. Food Sec. 2: 305-315. https://doi.org/10.1007/s12571-010-0085-0
  2. [FAO] Food and Agriculture Organization of the United Nations (2012 onwards). Value of Agricultural Production. URL http://www.fao.org/faostat/en/#data/QV [1 March 2016].
  3. Gioria, R., Espinha, L. M., Rezende, J. A. M., Gaspar, J. O. and Kitajima, E. W. 2002. Limited movement of Cucumber mosaic virus (CMV) in yellow passion flower in Brazil. Plant Pathol. 51: 127-133. https://doi.org/10.1046/j.1365-3059.2002.00678.x
  4. Kang, D. K., Kondo, T., Shin, J. H., Shin, H. Y., Sung, J. H., Kang, S. G. and Chang, M. U. 2003. Chinese yam necrotic mosaic virus isolated from Chinese yam in Korea. Res. Plant Dis. 9: 107-115. (In Korean) https://doi.org/10.5423/RPD.2003.9.3.107
  5. Kenyon, L., Shoyinka, S. A., Hughes, J. A. and Odu, B. O. 2001. An overview of viruses infecting Dioscorea yams in sub-Saharan Africa. In: Plant Vology in Sub-Saharan Africa: Proceeding of a Conference Organized by IITA, eds. by J. A. Hughes and B. O. Odu, pp. 432-493. 4-8 June, 2001, International Institute of Tropical Agriculture, Ibadan, Nigeria.
  6. Kwon, S. J., Cho, I. S., Choi, S. K., Yoon, J. Y. and Choi, G. S. 2016b. Complete sequence analysis of a Korean isolate of Chinese yam necrotic mosaic virus and generation of the virus specific primers for molecular detection. Res. Plant Dis. 22: 194-197. https://doi.org/10.5423/RPD.2016.22.3.194
  7. Kwon, S. J., Cho, I. S., Yoon, J. Y., Choi, S. K. and Choi, G. S. 2016a. First report of Broad bean wilt virus 2 in Dioscorea opposita Thunb. in Korea. Plant Dis. 100: 538.
  8. Lan, P., Li, F., Wang, M. and Li, R. 2015. Complete genome sequence of a divergent strain of Japanese yam mosaic virus from China. Arch. Virol. 160: 573-576. https://doi.org/10.1007/s00705-014-2231-z
  9. Lee, J. H., Son, C. G., Kwon, J. B., Nam, H. H., Kim, Y., Lee, S. H., Zhao, F. and Moon, J. S. 2016. Complete genome sequence of Chinese yam necrotic mosaic virus from Dioscorea opposita in the Republic of Korea. Genome Announc. 4: e00778-16.
  10. Lee, S. H., Lee, J. B., Kim, S. M., Choi, H. S., Park, J. W., Lee, J. S., Lee, K. W. and Moon, J. S. 2004. The incidence and distribution of viral diseases in pepper by cultivation types. Res. Plant Dis. 10: 231-240. (In Korean) https://doi.org/10.5423/RPD.2004.10.4.231
  11. Mambole, I. A., Bonheur, L., Dumas, L. S., Filloux, D., Gomez, R. M., Faure, C., Lange, D., Anzala, F., Pavis, C., Marais, A., Roumagnac, P., Candresse, T. and Teycheney, P. Y. 2014. Molecular characterization of yam virus X, a new potexvirus infecting yams (Dioscorea spp) and evidence for the existence of at least three distinct potexviruses infecting yams. Arch. Virol. 159: 3421-3426. https://doi.org/10.1007/s00705-014-2211-3
  12. Mantell, S. H. and Haque, S. Q. 1978. Incidence of internal brown spot disease in White Lisbon yams (Dioscorea alata) during storage. Exp. Agric. 14: 167-172. https://doi.org/10.1017/S0014479700008589
  13. Menzel, W., Thottappilly, G. and Winter, S. 2014. Characterization of an isometric virus isolated from yam (Dioscorea rotundata) in Nigeria suggests that it belongs to a new species in the genus Aureusvirus. Arch. Virol. 159: 603-606. https://doi.org/10.1007/s00705-013-1845-x
  14. Mumford, R. A. and Seal, S. E. 1997. Rapid single-tube immunocapture RT-PCR for the detection of two yam potyviruses. J. Virol. Methods 69: 73-79. https://doi.org/10.1016/S0166-0934(97)00141-9
  15. Wang, M., Li, F., Zhou, G., Lan, P., Xu, D. and Li, R. 2015. Molecular detection and characterization of Chinese yam mild mosaic virus isolates. J. Phytopathol. 163: 1036-1040. https://doi.org/10.1111/jph.12337