Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Detection of Barley yellow mosaic virus from Soil Using Nested PCR

Nested PCR 기법을 이용한 토양으로부터 Barley yellow mosaic virus 검출

  • Lee, Joong-Hwan (Institute for Bioresources Research, Gyeongsangbuk-do Agricultural Research & Extension Services) ;
  • Son, Chang-Gi (Institute for Bioresources Research, Gyeongsangbuk-do Agricultural Research & Extension Services) ;
  • Kwon, Joong-Bae (Institute for Bioresources Research, Gyeongsangbuk-do Agricultural Research & Extension Services) ;
  • Nam, Hyo-Hun (Institute for Bioresources Research, Gyeongsangbuk-do Agricultural Research & Extension Services) ;
  • Kim, Yeong-Tae (Institute for Bioresources Research, Gyeongsangbuk-do Agricultural Research & Extension Services) ;
  • Lee, Bong-Choon (Crop Foundation Division, National Institute of Crop Science, Rural Development Administration) ;
  • Shin, Dong-Bum (Crop Cultivation and Environment Research Division, National Institute of Crop Science, Rural Development Administration)
  • 이중환 (경상북도농업기술원 생물자원연구소) ;
  • 손창기 (경상북도농업기술원 생물자원연구소) ;
  • 권중배 (경상북도농업기술원 생물자원연구소) ;
  • 남효훈 (경상북도농업기술원 생물자원연구소) ;
  • 김영태 (경상북도농업기술원 생물자원연구소) ;
  • 이봉춘 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 신동범 (농촌진흥청 국립식량과학원 재배환경과)
  • Received : 2016.10.31
  • Accepted : 2017.02.14
  • Published : 2017.03.31
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Abstract

Barley yellow mosaic virus (BaYMV), which is transmitted by the root-inhabiting protist Polymyxa graminis, causes a soil-borne disease. In this study, we detected BaYMV from soil using two-step nested polymerase chain reaction (PCR). Specific primers based on a coat protein region of BaYMV segment RNA1 were used in the first round of amplification. Based on the sequenced amplicon, an inner primer was designed for the second round of amplification. A PCR product of 372 bp exhibited 98%-100% nucleotide sequence identity with the coat protein region of BaYMV segment RNA1. In this study, we propose an easy method for the detection of BaYMV from soil, may considerably assist in accurate fungus-transmitted virus diagnosis and subsequent disease forecasting. This is the first report on the detection of BaYMV from soil.

2단계의 nested PCR 방법을 이용하여 보리 및 벼 재배 토양에서 Barley yellow mosaic virus (BaYMV)를 검출하였다. BaYMV 분절 RNA1 외피단백질 영역의 특이 프라이머로 1차 PCR을 하고 내부서열로부터 작성된 프라이머로 2차 PCR을 실시하여 확보된 372 bp의 PCR 산물이 BaYMV 외피단백질 영역과 98%-100% 염기서열이 일치하여 BaYMV를 검출할 수 있음을 확인하였다. 이 결과는 토양으로부터 BaYMV 검출에 관한 최초의 보고이며 토양전염성 바이러스의 정확한 진단과 예찰에 적용될 수 있을 것으로 생각한다.

Keywords

References

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