DOI QR코드

DOI QR Code

Antithrombotic Activity of Extracts from the Aromatic Herb Elsholtzia splendens

  • Kim, Won Shik (Department of Clinical Laboratory Science, Daejeon Health Institute of Technology) ;
  • Lim, Yong (Department of Clinical Laboratory Science, Dong-Eui University)
  • 투고 : 2017.08.08
  • 심사 : 2017.08.28
  • 발행 : 2017.09.30

초록

Elsholtzia splendens, which grows on moist soil of mountainous regions, is widely distributed at all regions of Korea, especially at Mountain Ji ri. It is categorized as a Labiatae plant which is dried aerial part. It has the following medicinal properties; removal of fever, alleviation of pain, a good antiphlogistic agent as well as antibacterial effects. However, the effects of E. splendens on thrombosis and platelet activation are not precisely understood. We performed this study to develop antithrombotic agents from oriental medicine herb extracts. E. splendens inhibited platelet aggregation induced by arachidonic acid and U46619 in a concentration dependent manner. E. splendens did not show an effect on anticoagulation as determined by prothrombin time (PT) or activated partial thromboplastin time (aPTT). We also tested the effects of E. splendens using a carotid artery thrombosis rat model induced by 35% $FeCl_3$ treatment. E. splendens significantly inhibited thrombus weight compared with the control group. These results show that E. splendens may be developed as a potential antiplatelet activity agent for treatment of cardiocerebrovascular disease and atherosclerosis.

키워드

참고문헌

  1. Burggraf D, Vosko MR, Schubert M, Stassen JM, Hamann GF. Different therapy options protecting microvasculature after experimental cerebral ischaemia and reperfusion. Thrombosis and Haemostasis. 2010. 103: 891-900. https://doi.org/10.1160/TH09-07-0500
  2. Chen C, Yang FQ, Zhang Q, Wang FQ, Hu YJ, Xia ZN. Natural products for antithrombosis. Evidence-Based Complementary and Alternative Medicine. 2015: 876426.
  3. Heo J. Donguibogam. Namsandang. 2000. 660.
  4. Jin YR, Im JH, Park ES, Cho MR, Han XH, Lee JJ, Lim Y, Kim TJ, Yun YP. Antiplatelet activity of epigallocatechin gallate is mediated by the inhibition of PLCgamma2 phosphorylation, elevation of PGD2 production, and maintaining calcium-ATPase activity. Journal of Cardiovascular Pharmacology. 2008. 51: 45-54. https://doi.org/10.1097/FJC.0b013e31815ab4b6
  5. Liu S, Jiang S, Wu Z, Lv L, Zhang J, Zhu Z, Wu S. Identification of inhibitors of the HIV-1 gp41 six-helix bundle formation from extracts of Chinese medicinal herbs Prunella vulgaris and Rhizoma cibotte. Life Sciences. 2002. 71: 1779-1791. https://doi.org/10.1016/S0024-3205(02)01939-2
  6. Mine Y, Wong AHK, Jiang B. Fibrinolytic enzymes in Asian traditional fermented foods. Food Research International. 2005. 38: 243-250. https://doi.org/10.1016/j.foodres.2004.04.008
  7. Mizuno T, Sugimoto M, Matsui H, Hamada M, Shida Y, Yoshioka A. Visual evaluation of blood coagulation during mural thrombogenesis under high shear blood flow. Thrombosis Research. 2008. 121: 855-864. https://doi.org/10.1016/j.thromres.2007.07.019
  8. Park ES, Lim Y, Lee SH, Kwon BM, Yoo HS, Hong JT, Yun YP. Antiplatelet activity of obovatol, a biphenolic component of Magnolia Obovata, in rat arterial thrombosis and rabbit platelet aggregation. Journal of Atherosclerosis and Thrombosis. 2011. 18: 659-669. https://doi.org/10.5551/jat.7427
  9. Swenson S, Markland FS Jr. Snake venom fibrin(ogen)olytic enzymes. Toxicon. 2005. 45: 1021-1039. https://doi.org/10.1016/j.toxicon.2005.02.027
  10. Wolberg AS, Campbell RA. Thrombin generation, fibrin clot formation and hemostasis. Transfusion and Apheresis Science. 2008. 38: 15-23. https://doi.org/10.1016/j.transci.2007.12.005
  11. Wolberg AS. Thrombin generation and fibrin clot structure. Blood Reviews. 2007. 21: 131-142. https://doi.org/10.1016/j.blre.2006.11.001