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Study on sampling methods for mold from indoor air in domestic environment

국내 환경에서 실내 부유진균 포집 방법 연구

  • Ahn, Geum Ran (Department of Microbiology, Dankook University) ;
  • Kim, Bo Young (Department of Microbiology, Dankook University) ;
  • Kim, Ji Eun (Department of Microbiology, Dankook University) ;
  • Son, Bu Sun (Department of Environmental Health Science, Soonchunhyang University) ;
  • Park, Moo-Kyun (Department of Otorhinolaryngology, College of Medicine, Seoul National University College of Medicine) ;
  • Kim, Sung-Yeon (Indoor Environment & Noise Research Division, National Institute of Environmental Research) ;
  • Kwon, Myung-Hee (Indoor Environment & Noise Research Division, National Institute of Environmental Research) ;
  • Kim, Seong Hwan (Department of Microbiology, Dankook University)
  • 안금란 (단국대학교 자연과학대학 미생물학과) ;
  • 김보영 (단국대학교 자연과학대학 미생물학과) ;
  • 김지은 (단국대학교 자연과학대학 미생물학과) ;
  • 손부순 (순천향대학교 환경보건학과) ;
  • 박무균 (서울대학교 의과대학 이비인후과) ;
  • 김성연 (국립환경과학원 생활환경연구과) ;
  • 권명희 (국립환경과학원 생활환경연구과) ;
  • 김성환 (단국대학교 자연과학대학 미생물학과)
  • Received : 2016.12.07
  • Accepted : 2016.12.23
  • Published : 2017.03.31

Abstract

Mold is one of the important bio-aerosols affecting human health in the indoor environment. To manage mold contamination, it is necessary to use an appropriate method for its detection and enumeration. Recently, the impaction method of ISO 16000-18 has been established as one of methods to detect and enumerate molds in air. To investigate the general use of the impaction method for mold detection in domestic indoor environments, the suitability of the method was assessed using different antibiotics, media and air samplers. All of the three antibiotics tested - ampicillin, chloramphenicol and streptomycin - showed inhibitory effects on bacterial colony formation on MEA and DG-18 media, without inhibiting mold growth. Of these three antibiotics, ampicillin was the most effective. There was no statistical difference between MEA and DG-18 media in the measurement of mold concentration. The formation of discriminative colony morphology was more apparent in DG-18 media. No significant difference in the measurement of mold concentration was found between Andersen samplers and MAS-100NT samplers, which are two major samplers introduced in Korea.

Keywords

Acknowledgement

Supported by : 한국연구재단

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