Korean Circulation Journal

  • 제47권2호
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  • Pages.193-200
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  • 2017
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  • 1738-5520(pISSN)
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  • 1738-5555(eISSN)
대한심장학회 (The Korean Society of Cardiology)
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DOI QR Code

Inhibiting Cytochrome C Oxidase Leads to Alleviated Ischemia Reperfusion Injury

  • Yang, Zhaoyun (Department of Anesthesia, Xiang-Ya Second Hospital, Central South University) ;
  • Duan, Zhongxin (Department of Anesthesiology, the Second Affiliated Hospital, University of South China) ;
  • Yu, Tian (Department of Anesthesiology, Zunyi Medical College) ;
  • Xu, Junmei (Department of Anesthesia, Xiang-Ya Second Hospital, Central South University) ;
  • Liu, Lei (Department of Anesthesia, Xiang-Ya Second Hospital, Central South University)
  • 투고 : 2016.03.28
  • 심사 : 2016.07.07
  • 발행 : 2017.03.31
⟨ 이전 논문 다음 논문 ⟩

초록

Background and Objectives: The overall purpose of this study was to investigate the role of cytochrome C oxidase (CcO) in preventing ischemia reperfusion-induced cardiac injury through gaseous signaling molecule pathways. Materials and Methods: We used CcO inhibitor, potassium cyanide (KCN) to mimic the pre-treatment of gaseous signaling molecules in a global ischemia/reperfusion (IR) injury model in rats. Intracellular reactive oxygen species (ROS) was determined by measuring mitochondrial $H_2O_2$ and mitochondrial complex activity. Results: KCN pre-treatment led to decreased infarction area after IR injury and improved cardiac function. KCN pre-treated group challenged with IR injury was associated with reduced ROS production through inhibition of activity and not downregulation of CcO expression. In addition, KCN pre-treatment was associated with enhanced expression and activity of mitochondrial antioxidase, suggesting the role of CcO in regulating IR injury through oxidative stress. Conclusion: KCN pre-treatment reduced the severity of IR injury. The potential mechanism could be increased endogenous anti-oxidase activity and consequently, the enhanced clearance of ROS.

키워드

과제정보

연구 과제 주관 기관 : National Natural Science Foundation of China

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피인용 문헌

  1. The Effects of Potassium Cyanide on the Functional Recovery of Isolated Rat Hearts after Ischemia and Reperfusion: The Role of Oxidative Stress vol.2018, pp.None, 2018, https://doi.org/10.1155/2018/5979721
  2. Sirt1 Activation by Post-ischemic Treatment With Lumbrokinase Protects Against Myocardial Ischemia-Reperfusion Injury vol.9, pp.None, 2017, https://doi.org/10.3389/fphar.2018.00636