Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Reserpine treatment activates AMP activated protein kinase (AMPK)

  • Park, Rackhyun (Division of Biological Science and Technology, Yonsei University) ;
  • Lee, Kang Il (Division of Biological Science and Technology, Yonsei University) ;
  • Kim, Hyunju (Division of Biological Science and Technology, Yonsei University) ;
  • Jang, Minsu (Division of Biological Science and Technology, Yonsei University) ;
  • Ha, Thi Kim Quy (Korea Bioactive Natural Material Bank, College of Pharmacy, Seoul National University) ;
  • Oh, Won Keun (Korea Bioactive Natural Material Bank, College of Pharmacy, Seoul National University) ;
  • Park, Junsoo (Division of Biological Science and Technology, Yonsei University)
  • Received : 2017.01.18
  • Accepted : 2017.05.04
  • Published : 2017.09.29
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Abstract

Reserpine is a well-known medicine for the treatment of hypertension, however the role of reserpine in cell signaling is not fully understood. Here, we report that reserpine treatment induces the phosphorylation of AMP activated protein kinase (AMPK) at threonine 172 (T172) in PC12 cells. Phosphorylation of AMPK T172 is regulated by upstream signaling molecules, and the increase of phospho-T172 indicates that AMPK is activated. When we examined the FOXO3a dependent transcription by using the FHRE-Luc reporter assay, reserpine treatment repressed the FHRE-Luc reporter activity in a dose dependent manner. Finally, we showed that reserpine treatment induced the phosphorylation of AMPK as well as cell death in MCF-7 cells. These results suggest that AMPK is a potential cellular target of reserpine.

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