Journal of the Korean Data and Information Science Society

The Korean Data and Information Science Society (한국데이터정보과학회)
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Molecular genetic evaluation of gorals(naemorhedus caudatus raddeanus) genetic resources using microsatellite markers

초위성체 마커를 이용한 산양의 분자유전학적 고찰

  • Seo, Joo Hee (The Graduate School of Future Convergence Technology, Department of Genomic Informatics, Hankyong National University) ;
  • Lee, Yoonseok (Genomic Informatics Center, Hankyong National University) ;
  • Jeon, Gwang Joo (Genomic Informatics Center, Hankyong National University) ;
  • Kong, Hong Sik (Genomic Informatics Center, Hankyong National University)
  • 서주희 (한경대학교 미래융합기술대학원 유전체정보전공) ;
  • 이윤석 (한경대학교 유전정보연구소) ;
  • 전광주 (한경대학교 유전정보연구소) ;
  • 공홍식 (한경대학교 유전정보연구소)
  • Received : 2017.05.10
  • Accepted : 2017.09.07
  • Published : 2017.09.30
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Abstract

In this study, genotyping was executed by using 13 microsatellite markers for genetic diversity of 224 Gorals (Saanen(88), Laoshan(67), Toggenburg(32), Alpine(12), Anglonubian(9), Jamnapari(7) and Black Bengal(4)). The number of alleles was observed 4 (INRA005) to 18 (SRCRSP23) each markers. Observed heterozygostiy ($H_{obs}$), expected heterozygosity ($H_{\exp}$) and polymorphism information content (PIC) were observed 0.482 to 0.786, 0.476 to 0.923, and 0.392 to 0.915, respectively. Principal Components Analysis(PCoA) results were similar to the results of FCA. NE-I(on-exclusion probability for identity of two unrelated individuals) was estimated at $2.47{\times}10^{-15}$. In conclusion, this study shows the useful data that be utilized as a basic data of Gorals breeding and development.

본 연구는 산양 7 품종을 대상으로 (Saanen (88), Laoshan (67), Toggenburg (32), Alpine (12), Anglonubian (9), Jamnapari (7), Black Bengal (4)) 13종의 초위성체 마커 (microsatellite marker)를 활용하여 유전적 다형성 분석을 실시하였다. 대립유전자 수는 4개 (INRA005) 부터 18개 (SRCRSP23)까지 확인되었으며, 관측이형접합율 ($H_{obs}$)과 기대이형접합율 ($H_{\exp}$) 그리고 다형성 정보지수 (PIC) 값은 각각 0.482 ~ 0.786, 0.476 ~ 0.923 그리고 0.392 ~ 0.915로 나타났다. 품종별 유전적 거리를 확인하기 위하여 실시한 주성분분석 (PCoA) 결과는 요인대응분석 (FCA) 분석과 유사한 결과를 보였으며, 동일개체출현빈도는 $2.47{\times}10^{-15}$으로 확인되었다. 따라서 본 연구 결과는 산양 품종 개량 및 보존에 있어 기초자료로써 유용한 자료로 활용 가능 할 것으로 사료된다.

Keywords

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