Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Reduction of Sulfur Compounds Produced from Swine Manure, Using Brevundimonas diminuta

Brevundimonas diminuta를 이용한 돈분뇨에서 발생되는 황화합물의 저감

  • Oh, Min-Hwan (Department of Environmental and Energy Engineering, The University of Suwon) ;
  • Lee, Eun-Young (Department of Environmental and Energy Engineering, The University of Suwon)
  • 오민환 (수원대학교 환경에너지공학과) ;
  • 이은영 (수원대학교 환경에너지공학과)
  • Received : 2017.08.02
  • Accepted : 2017.09.19
  • Published : 2017.09.28
Download PDF
⟨ Previous Next ⟩

Abstract

Mixed substrate oil cakes are known to emit sulfides, ammonia, and amines. Microorganisms capable of removing odorous gases related to these sulfur compounds were isolated from colonies enriched in vials containing oil cakes and water. Activity tests for hydrogen sulfide and methyl mercaptan reduction were performed to measure the sulfide reduction ratio of the isolates. Control groups were prepared with 0.25 g oil cakes and 10 ml water in a 100-ml vial without inoculation. The experimental groups were prepared similarly, albeit with an inoculum. Hydrogen sulfide removal efficiency of >90% was observed for an isolate, which was identified as Brevundimonas diminuta by 16S rDNA sequence analysis. The sequence was deposited in the Korean Collection for Type Cultures under the accession number KCTC11724BP. B. diminuta could remove up to 200 ppmv standard hydrogen sulfide in 24 hours and demonstrated a maximum hydrogen sulfide and methyl mercaptan removal efficiency of 100% at 453 ppmv and 98 ppmv, respectively, in vial tests. Furthermore, B. diminuta cells in 20% (v/w) medium showed removal efficiency of >85% for sulfur compounds in an odor emission chamber for swine manure.

혼합유박은 황화물, 암모니아 그리고 아민이 발생하는 것으로 알려져 있다. 악취 저감 미생물을 선별하기 위해 물과 혼합유박이 포함된 바이얼을 농화 배양하였다. 황화물 저감 미생물의 분리를 위해 황화수소 및 메틸메르캅탄 저감 활성실험을 수행하였다. 대조군에는 100 ml 바이알에 혼합유박(0.25 g)과 증류수(10 ml)를 넣어 악취를 발생시켰으며 실험군에는 대조군 바이알과 같은 상태에서 분리균주를 접종하였다. 분리균주 중에서 황화합물의 저감효율이 가장 높은 균주를 일반적인 동정 방법인 16S rRNA sequence 분석으로 동정 결과 Brevundimonas diminuta로 동정되었으며 KCTC에 기탁하여 기탁번호 KCTC11724BP를 부여받았다. B. diminuta는 황화수소 표준가스를 200 ppmv까지 24시간내에 모두 제거하였다. 또한, 황화수소와 메틸머캅탄의 최대 제거 효율은 바이알 실험에서 453 ppmv과 98 ppmv에서 각각 100% 효율을 나타냈다. 또한 돈분을 이용한 악취발생반응기에서는 접종량 20% (v/weight of swine manure)일 때 황화물 95% 이상 제거 효율을 나타냈다.

Keywords

References

  1. Heo D. 2005. A Study on the Direct Payment Pilot Project for Environmental Friendly Livestock Farming in Korea. Korea Rural Economic Institute.
  2. Lowe PD. 1995. Social issues and animal wastes: A European perspective. In: Proceedings of International Livestock Odor Conference. pp 168-171. Iowa State Univ. College of Agric., Ames.
  3. Rall GD, Wood AJ, Westcott RB, Dommert AR. 1970. Distribution of bacteria in feces of swine. Appl. Microbiol. 20: 789-792.
  4. Nuru S, Osbaldiston GW, Stowe EC, Walker D. 1972. Fecal microflora of healthy cattle and pigs, The Cornell Veterinarian LXII: 242-253.
  5. Salanitro JP, Blake IG, Muirhead PA. 1977. Isolation and identification of fecal bacteria from adult swine. Appl. Environ. Microbiol. 33: 79-84.
  6. Russell EG. 1979. Types and distribution of anaerobic bacteria in the large intestine of pigs. Appl. Environ. Microbiol. 37: 187-193.
  7. Mackie RI. 1994. Microbial production of odor components. In: Proc. of International Round Table on Swine Odor Control, 13-15 June at Ames, IA, USA, pp. 18-19.
  8. Zhu J. 2000. A review of microbiology in swine manure odor control. Agric. Ecosyst. Environ. 78: 93-106. https://doi.org/10.1016/S0167-8809(99)00116-4
  9. Oh CM. 2016. A study on the technological trends on the odor emission of pig manure. Master dissertation. Sungkyunkwan University, Suwon, Korea.
  10. Watkins BD, Hengemuehle SM, Person HL, Yokoyama M, Masten SJ. 1997. Ozonation of swine manure wastes to control odors and reduce the concentrations of pathogens and toxic fermentation metabolies, Ozone Sci. Eng. 19: 425-437. https://doi.org/10.1080/01919512.1997.10382869
  11. Wu JJ, Park SH, Hengeuehle SM, Yokoyama M, Person HL, Masen SJ. 1998. The effect of storage and ozonation on the physical, chemical, and biological characteristics of swine manure slurries. Ozone Sci. Eng. 20: 35-50. https://doi.org/10.1080/01919519808547289
  12. Lee EY. 2008. Problems and verification system of probiotics as livestock-environment improving agent produced and circulated. Korean J. Microbiol. Biotechnol. 36: 87-95.
  13. Chiang SH, Hsieh WH. 1995. Effect of direct-fed microorganisms on broiler growth performance and litter ammonia level. Asian-Aus. J. Anim Sic. 8: 159-162. https://doi.org/10.5713/ajas.1995.159
  14. Kim JH, Kim CH, Ko YD. 2001. Effect of dietary supplementation of fermented feed ($Bio-{\partial}{(R)}$) on performance of finishing pigs and fecal ammonia gas emission. J. Anim. Sci. & Technol. 43: 193-202.
  15. Jung, J.H., Hong, S.M., Kim, H.Y., Meng, Q.W., and Kim, I.H. 2010. Effect of probiotics in diet on growth performance, nutrient digestibility, fecal microbial count, noxious gases emission from the feces, and blood profile in early-finishing pigs. J. Anim. Sci. & Technol. 52: 23-28. https://doi.org/10.5187/JAST.2010.52.1.023
  16. Hayes ET, Leek ABG, Curran TP, Dodd VA, Carton OT, Beattie VE, et al. 2004. The influence of diet crude protein level on odour and ammonia emissions from finishing pig houses. Biores. Tech. 91: 309-315. https://doi.org/10.1016/S0960-8524(03)00184-6
  17. Zhu J, Riskowski GL, Torremorell MT. 1998. Volatile fatty acids as odor indicators in swine manure-A critical review. Trasactions of the ASAE. 42: 175-182.
  18. National Institute of Environmental Research. 2007. Official odor test method.
  19. Hongoh Y, Yuzawa H, Ohkuma M, Kudo T. 2003. Evaluation of primers and PCR conditions for the analysis of 16S rRNA genes from a natural enviroment. FEMS Microbiol. 221: 299-304. https://doi.org/10.1016/S0378-1097(03)00218-0
  20. Sim HS, Kim MD. 2016. Antipathogenic activity of Bacillus amyloliquefaciens isolated from Korean traditional rice wine. Korean J. Microbiol. Biotechnol. 44: 98-105. https://doi.org/10.4014/mbl.1511.11005
  21. Lee EY, Lee SJ. 2010. Emission characterization of ammonia produced from swine nightsoil. Korean J. Microbiol. Biotechnol. 38: 308-314.
  22. Kwon HG, Jung JO. 2007. Isolation and characteristics of novel ammonia oxidizing bacteria Brevundimonas diminuta. Korean J. Env. Hlth. 33: 293-298.
  23. Hassan SHA, Van GSW, Kim SM, Yoon SH, Joo JH, Shin BS, et al. 2010. Isolation and characterization of Acidithiobacillus caldus from a sulfur-oxidizing bacterial biosensor and its role in detection of toxic chemicals. J. Microbiol. Methods. 82: 151-155. https://doi.org/10.1016/j.mimet.2010.05.008
  24. Stefanie M, Jorge V, David H, Mark D. 2011. Sulfur metabolism in the extreme acidophile Acidithiobacillus caldus. Frontiers in Microbiology 2: 1664-1681.