Journal of Korean Society of Occupational and Environmental Hygiene (한국산업보건학회지)

Korean Industrial Hygiene Association (한국산업보건학회)
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Protective Effect of NMDA Receptor Antagonist on the Neurotoxicity Induced by Lead as an Environmental Pollutant

환경오염원인 납의 신경독성에 대한 NMDA 수용체 길항제의 보호 효과

  • Kim, Young-Wo (Sanbon Hospital, School of Medicine, Wonkwang University) ;
  • Rim, Yo-Sup (Department of Bioenvironmenmental Science, Sunchon National University) ;
  • Seo, Young Mi (Department of Nursing Seonam University)
  • 손영우 (원광대학교 의과대학 산본병원) ;
  • 임요섭 (순천대학교 생명산업과학대학) ;
  • 서영미 (서남대학교 간호학과)
  • Received : 2017.09.01
  • Accepted : 2017.09.23
  • Published : 2017.09.30
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Abstract

Objectives: This study was performed to evaluate the neurototoxicity of the environmental pollutant lead acetate(LA) and the protective effect of the D-2-amino-5-phosphonovaleric acid(APV), N-methyl-D-aspartate(NMDA) receptor antagonist on LA-induced cytotoxicity in cultured C6 glioma cells. Materials and Methods: For this study, cell viability in cultured C6 glioma cells was assessed by XTT assay and antioxidative effect, such as lactate dehydrogenase(LDH) activity, by LDH detection kit. Results: LA significantly decreased cell viability in a dose-dependent manner, and the XTT50 value was determined to be 33.3 uM of LA. The cytotoxicity of LA was deemed highly toxic according to Borenfreund and Puerner's toxic criteria. The vitamin E antioxidant significantly increased cell viability damaged by LA-induced cytotoxicity in these cultures. For the protective effect of APV on LA-induced cytotoxicity, APV significantly increased not only cell viability, but also inhibition of LDH activity. From these results, it is suggested that oxidative stress is involved in the neurotoxicity of LA, and APV effectively protected against LA-induced cytotoxicity via an antioxidative effect as an inhibotory activity of LDH. Conclusions: Natural resources like APV may be putative therapeutic agents for the toxic diminution of environmental pollutants such as LA correlated with oxidative stress.

Keywords

References

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