Journal of Microbiology and Biotechnology

  • 제27권9호
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  • Pages.1586-1592
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  • 2017
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Isolation and Characterization of Some Promoter Sequences from Leuconostoc mesenteroides SY2 Isolated from Kimchi

  • Park, Ji Yeong (Industrial Technology Research Group, World Institute of Kimchi (an Annex of Korea Food Research Institute)) ;
  • Jeong, Seon-Ju (College of Pharmacy, Catholic University of Daegu) ;
  • Kim, Jeong A (Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University) ;
  • Kim, Jeong Hwan (Division of Applied Life Science (BK21 Plus), Graduate School, Gyeongsang National University)
  • 투고 : 2017.03.16
  • 심사 : 2017.06.26
  • 발행 : 2017.09.28
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초록

Some promoters were isolated and characterized from the genome of Leuconostoc mesenteroides SY2, an isolate from kimchi, a Korean traditional fermented vegetable. Chromosomal DNA of L. mesenteroides SY2 was digested with Sau3AI and ligated with BamHI-cut pBV5030, a promoter screening vector containing a promoterless cat-86. Among E. coli transformants (TFs) resistant against Cm (chloramphenicol), 17 were able to grow in the presence of $1,000{\mu}g/ml$ Cm and their inserts were sequenced. Transcription start sites were examined for three putative promoters (P04C, P25C, and P33C) by primer extension. Four putative promoters were inserted upstream of a promoterless ${\alpha}$-amylase reporter gene in $pJY15{\alpha}$. ${\alpha}$-Amylase activities of E. coli TFs containing $pJY15{\alpha}$ (control, no promoter), $pJY03{\alpha}$ ($pJY15{\alpha}$ with P03C), $pJY04{\alpha}$ (with P04C), $pJY25{\alpha}$ (with P25C), and $pJY33{\alpha}$ (with P33C) were 66.9, 78.7, 122.1, 70.8, and 99.3 U, respectively. Cells harboring $pJY04{\alpha}$ showed 1.8 times higher activity than the control. Some promoters characterized in this study might be useful for construction of food-grade expression vectors for Leuconostoc sp. and related lactic acid bacteria.

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참고문헌

  1. Caplice E, Fitzgerald GF. 1999. Food fermentations: role of microorganisms in food production and preservation. Int. J. Food Microbiol. 50: 131-149. https://doi.org/10.1016/S0168-1605(99)00082-3
  2. Settanni L, Corsetti A. 2008. Application of bacteriocins in vegetable food biopreservation. Int. J. Food Microbiol. 121: 123-138. https://doi.org/10.1016/j.ijfoodmicro.2007.09.001
  3. Morello E, Bermudez-Humaran LG, Llull D, Sole V, Miraglio N, Langella P, Poquet I. 2008. Lactococcus lactis, an efficient cell factory for recombinant protein production and secretion. J. Mol. Microbiol. Biotechnol. 14: 48-58. https://doi.org/10.1159/000106082
  4. Thakur K, Tomar SK, De S. 2016. Lactic acid bacteria as a cell factory for riboflavin production. Microb. Biotechnol. 9: 441-451. https://doi.org/10.1111/1751-7915.12335
  5. Kleerebezem M, Hugenholtz J. 2003. Metabolic pathway engineering in lactic acid bacteria. Curr. Opin. Biotechnol. 14: 232-237. https://doi.org/10.1016/S0958-1669(03)00033-8
  6. Shareck J, Choi Y, Lee B, Miquez CB. 2004. Cloning vectors based on cryptic plasmids isolated from lactic acid bacteria: their characteristics and potential applications in biotechnology. Crit. Rev. Biotechnol. 24: 155-208. https://doi.org/10.1080/07388550490904288
  7. Jeong SJ, Park JY, Lee HJ, Kim JH. 2007. Characterization of pFMBL1, a small cryptic plasmid isolated from Leuconostoc mesenteroides SY2. Plasmid 57: 314-323. https://doi.org/10.1016/j.plasmid.2006.09.003
  8. Bojovic B, Diordjevic G, Topisirovic L. 1991. Improved vector for promoter screening in lactococci. Appl. Environ. Microbiol. 57: 385-388.
  9. Nakamura LK. 1981. Lactobacillus amylovorus, a new starchhydrolyzing species from cattle waste-corn fermentations. Int. J. Syst. Bacteriol. 31: 56-63. https://doi.org/10.1099/00207713-31-1-56
  10. Luchansky JB, Tenn MC, Klaenhammer TR. 1991. Molecular cloning and deoxyribonucleic acid polymorphisms in Lactobacillus acidophilus and Lactobacillus gasseri. J. Dairy Sci. 74: 3293-3302. https://doi.org/10.3168/jds.S0022-0302(91)78515-9
  11. Dower WJ, Miller JF, Ragsdale CW. 1988. High efficiency transformation of E. coli by high voltage electroporation. Nucleic Acids Res. 16: 6127-6145. https://doi.org/10.1093/nar/16.13.6127
  12. Jeong JJ, Kim TY, Kim JH. 1997. Cloning of the entire gene encoding the 140-kDa $\alpha$-amylase of Lactobacillus amylovorus and expression in Escherichia coli and Lactococcus lactis. J. Microbiol. Biotechnol. 7: 293-298.
  13. Lee KW, Park JY, Kim GM, Kwon GH, Park JY, Lee MR, et al. 2008. Expression of alpha-amylase gene from Bacillus licheniformis in Lactobacillus brevis 2.14. J. Food Sci. Nutr. 13: 190-195.
  14. van der Vossen JM, van der Lelie D, Venema G. 1987. Isolation and characterization of Streptococcus cremoris Wg2- specific promoters. Appl. Environ. Microbiol. 53: 2452-2457.
  15. de Vos WM. 1999. Gene expression systems for lactic acid bacteria. Curr. Opin. Microbiol. 2: 289-295. https://doi.org/10.1016/S1369-5274(99)80050-2
  16. Jeong DW, Choi YC, Lee JM, Kim JH, Lee JH, Kim KH, et al. 2004. Isolation and characterization of promoters from Lactococcus lactis ssp. cremoris LM0230. Food Microbiol. 23: 82-89.
  17. Solaiman DK, Somkuti GA. 1997. Isolation and characterization of transcription signal sequences from Streptococcus thermophilus. Curr. Microbiol. 34: 216-219. https://doi.org/10.1007/s002849900171
  18. Park WJ, Lee KH, Lee JM, Lee HJ, Kim JH, Lee JH, et al. 2004. Characterization of pC7 from Lactobacillus paraplantarum C7 derived from kimchi and development of lactic acid bacteria-Escherichia coli shuttle vector. Plasmid 52: 84-88. https://doi.org/10.1016/j.plasmid.2004.05.001
  19. Park JY, Jeong SJ, Sa HD, Lee JY, Liu X, Cho MJ, et al. 2015. Construction o f a shuttle v ector b ased on the small cryptic plasmid pJY33 from Weissella cibaria 33. Plasmid 79: 30-36. https://doi.org/10.1016/j.plasmid.2015.03.008
  20. Mierau I, Kleerebezem M. 2005. 10 years of the nisincontrolled gene expression system (NICE) in Lactococcus lactis. Appl. Microbiol. Biotechnol. 68: 705-717. https://doi.org/10.1007/s00253-005-0107-6
  21. Nguyen TT, Mathiesen G, Fredriksen L, Kittl R, Nguyen TH, Eijsink VGH, et al. 2011. A food-grade system for inducible gene expression in Lactobacillus plantarum using an alanine racemase-encoding selection marker. J. Agric. Food Chem. 59: 5617-5624. https://doi.org/10.1021/jf104755r
  22. Liu CQ, Su P, Khunajakr N, Deng YM, Sumual S, Kim WS, et al. 2005. Development of food-grade cloning and expression vectors for Lactococcus lactis. J. Appl. Microbiol. 98: 127-135. https://doi.org/10.1111/j.1365-2672.2004.02441.x

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