Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Comparative Studies of the Cytotoxic Effect of Four Different Sea Bream Species (Pagrus major, Acanthopagus schlegeli, Oplegnathus fasciatus, and Girella punctata)

4종 돔 추출물의 세포독성 효과 비교

  • Hwang, Seong Yeon (Division of Marine Bioscience, Korea Maritime and Ocean University) ;
  • Lim, Sun-Young (Division of Marine Bioscience, Korea Maritime and Ocean University)
  • 황성연 (한국해양대학교 해양생명과학부) ;
  • 임선영 (한국해양대학교 해양생명과학부)
  • Received : 2017.05.03
  • Accepted : 2017.07.29
  • Published : 2017.09.30
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Abstract

This study compared the cytotoxic effect of extracts from four different sea bream species (Pagrus major, Acanthopagus schlegeli, Oplegnathus fasciatus, and Girella punctata) in human cancer cell lines. Cytotoxic activity against the growth of human gastric adenocarcinoma (AGS) and HT-29 human colon cancer cell lines was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Treatment with acetone/methylene chloride (A+M) and methanol (MeOH) extracts from the four sea bream species dose-dependently increased cytotoxicity against the growth of AGS and HT-29 cancer cells (p < 0.05). As shown by a cell viability assay, treatment with A+M and MeOH extracts from red sea bream (P. major) had the highest cytotoxic effect (p < 0.05) among the sea bream species. The IC50 values of an 85% aqueous methanol (85% aq. MeOH) fraction from red sea bream (P. major) against AGS and HT-29 cancer cells was 0.33 and 1.58 mg/ml, respectively, suggesting that the 85% aq. MeOH fraction had the highest cytotoxic effect among the fractions (p < 0.05). Our results demonstrate that four different sea bream species exhibited cytotoxic activity, as well as high-quality amino acids and fatty acids. Among the sea bream species, red sea bream (P. major) showed the greatest cytotoxic effect. The results could be used to improve nutrition information available to consumers.

본 연구에서는 국내에서 주로 소비되고 있는 감성돔(A. schlegeli), 참돔(P. major), 돌돔(O. fasciatus) 및 벵에돔(G. punctata)에 대한 기능성 식품으로서 영양학적 가치를 평가하기 위하여 인체 위암세포(AGS)와 인체 결장암세포(HT-29)에 대한 세포 독성 효과를 비교 연구하였다. 4종의 돔 A+M 및 MeOH 추출물들을 AGS 암세포에 처리했을 때 A+M추출물의 경우 첨가농도 5 mg/ml에서 참돔(P. major)과 감성돔(A. schlegeli) 추출물은 80% 이상의 높은 세포독성 효과를 나타내었으며 그 다음으로 벵에돔(G. punctata)이 70%, 돌돔(O. fasciatus)이 53%의 세포독성 효과를 보였다. MeOH 추출물의 경우 참돔(P. major)과 돌돔(O. fasciatus) 추출물이 약 80% 이상의 세포독성 효과를 나타났으며, 이어서 감성돔(A. schlegeli)과 벵에돔(G. punctata) 추출물이 약 70% 정도의 세포독성 효과를 보였다. 4종의 돔의 HT-29 암세포에 대한 세포독성 효과는 AGS 암세포의 결과와 비교했을 때 세포독성 효과는 다소 낮았다. 참돔(P. major), 감성돔(A. schlegeli), 돌돔(O. fasciitis) 및 벵에돔(G. punctata)의 A+M 추출물의 $IC_{50}$ 값은 각각 3.35, 6.16, 6.17 및 4.47 mg/ml이었고 MeOH 추출물의 경우 $IC_{50}$ 값은 각각 3.61, 5.52, 2.07, 및 6.06 mg/ml이었다. 이상의 결과로부터 4종 돔 중에서 참돔(P. major) 추출물에 의한 세포독성 효과가 상대적으로 높아 참돔(P. major) 추출물을 용매 극성에 따라 분획하여 얻어진 n-hexane, 85% aq. MeOH, n-BuOH 및 water 분획물들에 의한 인체 암세포에 대한 세포독성 효과를 알아 본 결과 85% aq. MeOH 분획물에 의한 AGS 및 HT-29암세포에 대한 $IC_{50}$ 값은 각각 0.33 및 1.58 mg/ml로 나타났다. 따라서 참돔(P. major)의 85% aq. MeOH 분획물은 AGS 및 HT-29 세포 모두에서 가장 높은 세포독성 효과를 보여 85% aq. MeOH 분획물에 활성물질이 함유되어 있을 것으로 기대되고 본 연구 결과를 기초자료로 하여 향후 참돔의 다양한 생리활성 규명이 필요하다고 사료된다.

Keywords

References

  1. Bae, J. H. and Lim, S. Y. 2010. Metals and biochemical composition of four sea bream species (Acanthopagrus schlegelii Bleeker, Pargrus major Temminck and Schlegel, Oplegnathus fasciatus Kroyer and Girella punctata Gray). Philipp. Agric. Scientist 95, 102-108.
  2. Bae, J. H. and Lim, S. Y. 2011. Comparison between ordinary and dark muscle extracts of yellowtail (Seriola quinqueradiata) on chemical characteristics, antiproliferativeand antioxidant properties. J. Food Technol. 9, 99-105. https://doi.org/10.3923/jftech.2011.99.105
  3. Bae, J. H. and Lim, S. Y. 2012. Chemical composition, antiproliferative and antioxidant properties of lipid classes in ordinary and dark muscles from chub mackerel (Scomber japonicas). Food Chem. Toxicol. 50, 823-828. https://doi.org/10.1016/j.fct.2011.12.038
  4. Bae, J. S., Jung, J. M., An, C. M., Kim, J. W., Hwang, S. D., Kwon, M. G., Park, M. A., Kim, M. C. and Park, C. I. 2016. Piscidin: Antimicrobial peptide of rock bream, Oplegnathus fasciatus. Fish Shellfish Immunol. 51, 136-142. https://doi.org/10.1016/j.fsi.2016.02.010
  5. Denizot, F. and Lang, R. 1986. Rapid colorimetric assay for cell growth and survival. Modification to the tetazolium dye procedure giving improved sensitivity and reliablity. J. Immunol. Methods 89, 271-277. https://doi.org/10.1016/0022-1759(86)90368-6
  6. Hwang, W. I., Baik, N. G., Hwang, Y. K. and Lee, S. D. 1992. Antitumor and immunological effects of tuna extract. J. Kor. Soc. Food Nutr. 21, 353-366.
  7. Hwang, S. Y. and Lim, S. Y. 2015. Heavy metal contents and antioxidant activity and cytotoxic effect of red sea bream (Pagrus major): Comparative studies in domestic and imported red sea bream (Pagrus major). J. Life Sci. 25, 450-455. https://doi.org/10.5352/JLS.2015.25.4.450
  8. Jang, J. R., Choi, H. J., Kim, K. K. and Lim, S. Y. 2008. Effect of extracts from dried mackerel on antioxidant activity and inhibition of growth of human cancer cell lines. J. Life Sci. 18, 680-685. https://doi.org/10.5352/JLS.2008.18.5.680
  9. Jang, J. R., Kim, K. K., Mun, S. B. and Lim, S. Y. 2009. In vitro Anticancer and antioxidant effect of solvent extracts from tuna dried at low temperature vacuum. J. Life Sci. 19, 633-638. https://doi.org/10.5352/JLS.2009.19.5.633
  10. Kim, S. S., Rahimnejad, S., Song, J. W. and Lee, K. J. 2012. Comparison of growth performance and whole-body amino acid composition in red seabream (Pagrus major) fed free or dipeptide form of phenylalanine. Asian-Aust J. Anim. Sci. 25, 1138-1144. https://doi.org/10.5713/ajas.2012.12054
  11. Kong, C. S., Bak, S. S., Jung, K. O., Kil, J. H., Lim, S. Y. and Park, K. Y. 2005. Antimutagenic and anticancer effects of salted mackerel with various kinds of salts. J. Kor. Fish. Soc. 38, 281-285.
  12. Mahfouz, E. M., Sadek, R. R., Abdel-Latiel, W. M., Mosallem, F. A. H. and Hassan, E. E. 2014. The role of dietary and lifestyle factors in the development of colorectal cancer: case control study in Minia, Egypt. Cent. Eur. J. Public Health 22, 215-222. https://doi.org/10.21101/cejph.a3919
  13. Ministry for Food, Agriculture, Forestry and Fisheries (MIFAFF). 2012. Agricultural and forestry statistical yearbook, pp. 302-317, MIFAFF Press, Seoul, Korea.
  14. Na, N. 1997. The cardiac internuncial cell in Carassius auratus longsdorffi and other 19 teleost species: a fine-structural study. Arch. Histol. Cytol. 60, 405-419. https://doi.org/10.1679/aohc.60.405
  15. Venmathi Maran, B. A., Oh, S. Y., Soh, H. Y. and Choi, H. J. 2012. Caligus sclerotinosus (Copepoda: Caligidae), a serious pest of cultured red seabream Pagrus major (Sparidae) in Korea. Veter. Parasitol. 188, 355-361. https://doi.org/10.1016/j.vetpar.2012.03.023
  16. Yoo, M. H., Hwang, D. J., Yoon, Y. H., Jeong, G. S. and Go, H. J. 2003. Initial adaptation of related black sea bream, Acanthopagrus schlegeli in Gamak bay, southern coast in Korea. J. Jor. Fish. Soc. 36, 365-371.