Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Extract of Balloon-flower Inhibited In Vitro Angiogenesis in Human Umbilical Vein Endothelial Cells

도라지 추출물에 의한 인간 제대 정맥 내피 세포의 in vitro 혈관신생 억제

  • Yi, Eui-Yeun (Department of Molecular Biology, College of Natural Science, Pusan National University) ;
  • Kim, Yung-Jin (Department of Molecular Biology, College of Natural Science, Pusan National University)
  • 이의연 (부산대학교 분자생물학과) ;
  • 김영진 (부산대학교 분자생물학과)
  • Received : 2017.04.13
  • Accepted : 2017.05.10
  • Published : 2017.09.30
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Abstract

Angiogenesis is an essential step in tumoral growth and metastasis and is regulated by a balance between stimulators and inhibitors. Recently, antiangiogenic target therapy has shown promise as a new type of chemotherapy. Natural products have attracted widespread attention worldwide as a useful source of novel therapeutic compounds. The balloon-flower has long been used as a traditional medicinal material and food in Asia. In this study, we investigated whether extract of balloon-flower would inhibit in vitro angiogenesis and vascular-like network formation in human umbilical vein endothelial cells (HUVECs). The extract of Balloon-flower did not affect the viability of HUVECs. However, treatment with the Balloon-flower extract suppressed tube formation of HUVECs. In addition, after treatment with the Balloon-flower extract, cell migration decreased about 80%, and cell invasion was almost completely inhibited. Taken together, these results suggest that extract of Balloon-flower may have potential as an angiogenic inhibitor and that it could be developed as an anticancer agent.

혈관신생과정은 종양 형성과 이동에 필수적인 과정으로 촉진제와 저해제에 의하여 조절되며, 이러한 혈관신생과정의 저해는 새로운 항암치료 기법으로 이용하고 있다. 최근, 한약재와 식료품으로부터 추출한 천연물을 새로운 치료 물질로 널리 이용하고 있으며, 실제 in vitro 뿐만 아니라 in vivo 상에서도 항암 효과가 나타나는 것을 확인하였다. 그 중 도라지는 아시아에서 한약재와 식료품으로 오랫동안 사용 되어왔다. 본 연구에서는 도라지 추출물이 in vitro 상에서 인간 제대 정맥 내피 세포의 혈관신생을 억제하는 효과에 대해 조사하였다. 도라지 추출물은 세포독성 없이, 혈관 형성 및 이동, 침윤 현상을 모두 억제하는 효과를 보였다. 특히, 세포 이동은 80% 정도 감소 시켰으며 침윤 현상이 거의 나타나지 않는 것을 확인하였다. 이러한 결과를 토대로 도라지 추출물은 혈관신생 억제제로 이용할 수 있으며, 더 나아가 항암제로 개발될 수 있을 것이라 사료된다.

Keywords

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