생명과학회지 (Journal of Life Science)

  • 제27권9호
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  • Pages.994-1002
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  • 2017
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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고장초 추출 용매의 에탄올 함량에 따른 항산화, 항염증, 항알러지, 미백 활성 비교 분석

Comparative Analysis of Anti-oxidative, Anti-inflammatory, Anti-allergy, and Whitening Effects of Different Solvent Extracts from Zizania latifolia

  • 박세호 (계명대학교 자연과학연구소) ;
  • 이재열 (계명대학교 자연과학연구소) ;
  • 양선아 (계명대학교 식품가공학 전공)
  • Park, Se-Ho (Institute of Natural Science, Keimyung University) ;
  • Lee, Jae-Yeul (Institute of Natural Science, Keimyung University) ;
  • Yang, Seun-Ah (Major in Food Science and Technology, Keimyung University)
  • 투고 : 2017.08.04
  • 심사 : 2017.08.24
  • 발행 : 2017.09.30
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초록

본 연구에서는 고장초 줄기 ethanol 추출물 5종(10, 30, 50, 70, 90% Z. latifolia ethanol extract)에 대한 다양한 효능을 평가하여 원료제조를 위한 추출 조건을 최적화하고자 하였다. Z. latifolia ethanol extracts의 항산화능 평가결과, ethanol의 함량이 증가함에 따라 DPPH 및 ABTS 라디칼에 대한 $RC_{50}$ 값은 감소하여 소거능이 증가함을 확인하였다. Z. latifolia ethanol extracts의 항염증 평가 결과, ethanol의 함량이 증가함에 따라 lipopolysaccharide (LPS)로 처리한 RAW 264.7에서 nitric oxide (NO)의 생성 억제능이 농도의존적으로 증가되는 경향을 확인하였으며, $250{\mu}g/ml$의 농도에서의 NO 생성능 비교 시, 15.69, 14.13, 11.86, 8.64, $10.09{\mu}M$로 70% Z. latifolia ethanol extract에서 가장 높은 NO 억제 활성을 나타냈다. Z. latifolia ethanol extracts의 항알러지 효능을 평가하기 위하여 RBL-2H3 세포를 IgE-항원으로 활성화 시킨 결과, 10% Z. latifolia ethanol extract의 ${\beta}-hexosaminidase$ 방출 억제효과는 나타나지 않았으나, 30% Z. latifolia ethanol extract부터 농도의존적으로 ${\beta}-hexosaminidase$의 방출 억제효과를 확인할 수 있었다. 특히, $100{\mu}g/ml$ 추출물 처리 시 ${\beta}-hexosaminidase$ 방출은 각각 94.20, 57.10, 46.70, 36.38, 27.45%로 90% Z. latifolia ethanol extract의 항알러지 활성이 가장 우수하였다. Z. latifolia ethanol extracts의 미백 효능 평가 결과, ethanol 함량이 증가함에 따라 멜라닌 생성 억제 효과도 증가함을 확인할 수 있었으며, 특히 $500{\mu}g/ml$ 농도의 70%, 90% Z. latifolia ethanol extracts의 멜라닌 생성 억제능은 양성대조군인 arbutin ($250{\mu}M$)과 비슷한 효과를 나타내었다. 본 연구 결과, Z. latifolia ethanol extracts는 세포 독성이 없으며 피부 보호 효능과 관련된 다양한 활성이 우수함을 확인하였다. 이러한 원료 제조 조건에 대한 활성 결과는 향후 피부건강을 위한 기능성 식품 원료 제조를 위한 기초 결과로 활용될 것으로 기대된다.

This study was performed to evaluate the anti-oxidative, anti-inflammatory, anti-allergy, and whitening effects of Zizania latifolia ethanol extracts prepared from 5 different ethanol concentrations (10, 30, 50, 70, and 90%). As the ethanol concentration in the extraction solvent was increased, the radical scavenging activities also increased. The inhibitory activity of Z. latifolia ethanol extracts on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells tended to increase as the content of ethanol increased. The highest inhibitory activity was obtained with 70% ethanol extract. The antiallergy effects of Z. latifolia ethanol extracts were tested by measuring the release of ${\beta}-hexosaminidase$ in IgE-sensitized RBL-2H3 cells. The suppressive effect of Z. latifolia ethanol extracts increased in a dose-dependent manner as the proportion of ethanol increased, except for the 10% ethanol extract. Furthermore, the inhibitory effects of Z. latifolia ethanol extracts against melanin production in ${\alpha}-melanocyte$ stimulated hormone (MSH)-stimulated B16F0 cells increased as the ethanol ratio increased, and 70 and 90% ethanol extracts showed similar inhibitory activities to arbutin, a positive control, at $250{\mu}m$. The present study confirmed the efficacy of Z. latifolia ethanol extracts in various areas, demonstrating antioxidative, anti-inflammation, antiallergy, skin protective, and skin whitening effects, with no cytotoxicity. It could be used as a raw material in functional foods, as well as in cosmetics.

키워드

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