Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Anti-inflammatory Effects of Lemon Myrtle (Backhousia citriodora) Leaf Extracts in LPS-induced RAW 264.7 Cells

LPS로 유도된 RAW 264.7 세포에 대한 레몬 머틀 잎 추출물의 항염증 효과

  • Kim, Pan Kil (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology) ;
  • Jung, Kyung Im (Department of Food & Nutrition, Silla University) ;
  • Choi, Young Ju (Department of Food & Nutrition, Silla University) ;
  • Gal, Sang Wan (Department of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
  • 김판길 (경남과학기술대학교 제약공학과) ;
  • 정경임 (신라대학교 식품영양학과) ;
  • 최영주 (신라대학교 식품영양학과) ;
  • 갈상완 (경남과학기술대학교 제약공학과)
  • Received : 2017.06.09
  • Accepted : 2017.08.24
  • Published : 2017.09.30
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Abstract

Lemon myrtle (Backhousia citriodora) has been identified as one of the plants that are likely to undergo important commercial exploitation. This study was carried out to investigate the anti-inflammatory activities and nitric oxide synthase (iNOS) expression of hot water (LMW) and 80% ethanol (LME) extracts from lemon myrtle leaf in lipopolysaccharide-induced (LPS) RAW 264.7 cells. The total phenol content of LMW and LME was 207.44 and $331.54{\mu}g$ tannic acid equivalents (TAE)/mg, respectively (p<0.01). DPPH radical scavenging activities of LMW and LME were remarkably increased in a dose-dependent manner, and were about 90.69% and 92.50% at 0.5 mg/ml, respectively. Superoxide dismutase (SOD) activities of LMW and LME were 106.22% and 103.58% at 1 mg/ml, respectively. The highest activity (91.03%) of nitrite-scavenging was observed for LME at 1 mg/ml at pH 1.2, while the activity for LMW was about 81.03% under the same conditions (p<0.05). Anti-inflammatory effect was examined in LPS stimulated RAW 264.7 cells. Nitric oxide (NO) production were reduced to 35.41% and 78.39% by addition of LMW and LME at 0.5 mg/ml, respectively (p<0.05). LMW and LME reduced protein expression of inducible nitric oxide synthase (iNOS) in a dose-dependent manner (p<0.05). These results, we conclude that lemon myrtle may be a highly valuable natural product owing to its high-quality functional components as well as its anti-oxidant and anti-inflammatory activities.

본 연구에서는 레몬 머틀 잎 열수 및 에탄올 추출물을 이용하여 항산화 활성과 NO 소거능 및 RAW 264.7 세포에서의 항염증 활성에 미치는 영향을 확인하였다. 레몬 머틀 열수 및 에탄올 추출물의 총 페놀 함량은 각각 207.44와 $331.54{\mu}g\;TAE/mg$이었고, DPPH radical 소거능은 0.5 mg/ml 농도에서 각각 90.69%와 92.50%로 나타났으며, SOD 활성은 1 mg/ml 농도에서 각각 106.22%와 103.58%로 나타났다. 아질산염 소거능 분석에서는 열수와 에탄올 추출물 1 mg/ml 농도, pH 1.2에서 81.06%와 91.03%로 에탄올 추출물의 소거능이 높은 것으로 나타났다. 레몬머틀의 항염증 활성을 측정하기 위하여 대식세포에 LPS를 처리하여 세포 내 NO 생성을 유도한 후 열수 및 에탄올 추출물을 처리하여 각각의 추출물이 NO 활성에 미치는 영향을 분석한 결과 각 추출물 모두 농도 의존적으로 감소하였다. 또한 LPS에 의해 증가된 iNOS 단백질 발현 또한 농도 의존적으로 감소하였다. 이상의 결과에서와 같이 레몬 머틀 잎은 항산화 및 항염증 효과가 있는 것으로 나타났기에 천연물 소재로서의 활용도가 높을 것으로 생각된다.

Keywords

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