Journal of the korean academy of Pediatric Dentistry (대한소아치과학회지)

Korean Academy of Pediatric Dentistry (대한소아치과학회)
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Estimated Time of Biomineralization in Developing Rat Incisors

발생 중인 쥐 절치의 생물학적 광화 소요 시간

  • Park, Min Kyoung (Department of Pediatric Dentistry, College of Dentistry, Yonsei University) ;
  • Min, Soo-Young (Department of Pediatric Dentistry, College of Dentistry, Yonsei University) ;
  • Song, Je Seon (Department of Pediatric Dentistry, College of Dentistry, Yonsei University) ;
  • Lee, Jae-Ho (Department of Pediatric Dentistry, College of Dentistry, Yonsei University) ;
  • Jung, Han-Sung (Division in Anatomy and Developmental Biology, Department of Oral Biology, College of Dentistry, Yonsei University) ;
  • Kim, Seong-Oh (Department of Pediatric Dentistry, College of Dentistry, Yonsei University)
  • 박민경 (연세대학교 치과대학 소아치과학교실) ;
  • 민수영 (연세대학교 치과대학 소아치과학교실) ;
  • 송제선 (연세대학교 치과대학 소아치과학교실) ;
  • 이제호 (연세대학교 치과대학 소아치과학교실) ;
  • 정한성 (연세대학교 치과대학 구강생물학교실) ;
  • 김성오 (연세대학교 치과대학 소아치과학교실)
  • Received : 2016.06.13
  • Accepted : 2016.08.17
  • Published : 2017.05.31
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Abstract

The aim of this study was to estimate time of biomineralization in developmental stages of rat lower incisors. Eruption length was measured. Four stages of incisor development were identified on histologic and microscopic computerized tomography (micro-CT) sections: (1) preodontoblast, (2) dentin matrix secretion, (3) enamel matrix secretion, and (4) enamel calcification. The overall eruption rate of the rat lower incisor was $600{\pm}70{\mu}m/day$ ($mean{\pm}SD$; n = 12). The length of the enamel secretion was $4.59{\pm}0.75mm$ in histologic section, was $3.64{\pm}0.63mm$ in radiographic section, which converts to $180.4{\pm}30.0hours$, $145{\pm}25hours$ respectively (n = 24). These findings suggested that the four biomineralizing developmental stages of the rat incisor took only several days. The significance of this animal study was to provide understanding for the rapid biomineralization process of developing rat tooth germ by analysis of tooth forming period.

이 연구의 목적은 쥐의 하악 절치에서 생물학적 광화에 대한 발육 단계를 타임테이블로 수립하는데 있다. 맹출 길이가 측정되었고 조직학적 절편과 미세단층촬영기(microscopic computerized tomography) 단면으로 절치 발육의 4단계 (1) preodontoblast, (2) dentin matrix secretion, (3) enamel matrix secretion, 그리고 (4) enamel calcification을 확인하였다. 쥐의 하악 절치에서 총 맹출 속도는 $600{\pm}70{\mu}m/day$ ($mean{\pm}SD$; n = 12) 였다. 법랑질 분비 길이는 조직학적 절편에서 $4.59{\pm}0.75mm$, 방사선학적 단면에서 $3.64{\pm}0.63mm$였고 이를 각각 속도로 환산하면 $180.4{\pm}30.0hours$, $145{\pm}25hours$ 으로 나타났다(n = 24). 이러한 결과들은 쥐의 절치의 생물학적 광화가 일어나는 발육 4 단계가 단 며칠 만에 이루어진다는 것을 제시한다. 이번 동물 실험연구의 결과는 발육 중인 치배의 빠른 광물화 과정을 치아 발육 기간의 분석을 통해 이해할 수 있다는 것에 의의가 있다.

Keywords

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