International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
권호 표지
DOI QR코드

DOI QR Code

Study on Changes in Endogenous Stem Cells in the Salivary Gland of Streptozotocin-induced Diabetic Rats

  • Jung, Bo Hyun (Department of Anatomy, College of Dentistry and Research Institute of Oral Science, Gangneung-Wonju National University) ;
  • Lee, Hee Su (Department of Anatomy, College of Dentistry and Research Institute of Oral Science, Gangneung-Wonju National University) ;
  • Yoo, Ki-Yeon (Department of Anatomy, College of Dentistry and Research Institute of Oral Science, Gangneung-Wonju National University)
  • Received : 2017.06.02
  • Accepted : 2017.06.24
  • Published : 2017.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Type1 diabetes mellitus (DM) is generally known to be caused by destruction of insulin-producing pancreatic ${\beta}$ cells or an immune-related problem. Polydipsia is a representative symptom of DM, and it has been reported that this condition is closely related to xerostomia and is considered that hyposalivation from the salivary gland results in this phenomenon. Although various studies have reported that induction of diabetes reduces endogenous stem cells in other organs (heart, brain etc.), diabetes-related changes in endogenous stem cells in the salivary gland have not yet been well established. Therefore, in this study, to verify the change in salivary gland stem cells after diabetes, salivary gland tissues in the control and diabetes-induced groups were processed by histochemistry (Masson's trichrome staining) for morphological analysis, TUNEL assay for cell death, and immunohistochemistry (Ki-67 and c-Kit) for cell proliferation and maturation. Diabetes induced by STZ leads to vacuolization, apoptosis, and reduction in proliferating cells/salivary gland stem cells in salivary glands of rats. This result suggests that diabetes may be associated with reduction in salivary gland function such as degeneration and inhibition of regeneration in the salivary gland.

Keywords

References

  1. Atkinson MA, Eisenbarth GS, Michels AW. Type 1 diabetes. The Lancet. 2014;383:69-82. DOI:10.1016/S0140-6736(13)60591-7
  2. Mahay S, Adeghate, Lindley MZ, Rolph CE, Singh J. Streptozotocin-induced type 1 diabetes mellitus alters the morphology, secretory function and acyl lipid contents in the isolated rat parotid salivary gland. Molecular and cellular biochemistry. 2004;261:175-181. DOI:10.1023/B:MCBI.0000028753.33225.68
  3. Kim EJ, Diabetes, 1st ed., pp211-215, Korea medical book publishing company. Seoul, 1992.
  4. Moore PA, Guggenheimer J, Etzel KR, Weyant RJ, Orchard T. Type 1 diabetes mellitus, xerostomia, and salivary flow rates. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. 2001;92:281-291. DOI:10.1067/more.2001.117815
  5. Dodds MWJ, Yeh CK, Johnson DA. Salivary alterations in type 2 (non-insulin-dependent) diabetes mellitus and hypertension. Community Dent. Oral Epidemiol. 2000;28:373381. DOI:10.1034/j.1600-0528.2000.028005373.x
  6. Leite RS, Marlow NM, Fernandes JK. Oral health and type 2 diabetes. Am J Med Sci. 2013;345:271273. DOI:10.1097/MAJ.0b013e31828bdedf
  7. Fedirko NV, Kruglikov IA, Kopach OV, Vats JA, Kostyuk PG, Voitenko NV. Changes in functioning of rat submandibular salivary gland under streptozotocin-induced diabetes are associated with alterations of ca 2 signaling and ca 2 transporting pumps. Biochimica Et Biophysica Acta (BBA)-Molecular Basis of Disease. 2006;1762:294-303. DOI:10.1016/j.bbadis.2015.12.002
  8. Satoh K, Narita T, Matsuki-Fukushima M, Okabayashi K, Ito T, Senpuku H, Sugiya H. E2f1-deficient NOD/SCID mice have dry mouth due to a change of acinar/duct structure and the down-regulation of AQP5 in the salivary gland. Pflgers Archiv-European Journal of Physiology. 2013;465:271-281. DOI:10.1007/s00424-012-1183-y
  9. Slack JMW. Stem cells in epithelial tissues. Science. 2000;287:1431-1433. DOI:10.1126/science.287.5457.1431
  10. Potten CS, Booth C, Hargreaves D. The small intestine as a model for evaluating adult tissue stem cell drug targets. Cell Prolif. 2003;36:115-129. DOI:10.1046/j.1365-2184.2003.00264.x
  11. Suzuki A, Zheng Y, Kaneko S, Onodera M, Fukao K, Nakauchi H, Taniguchi H. Clonal identification and characterization of self-renewing pluripotent stem cells in the developing liver. J. Cell. Biol. 2002;156:173184. DOI:10.1083/jcb.200108066
  12. Boyd AS, Rodrigues NP,Lui KO, Fu X, Xu Y. Concise review: Immune recognition of induced pluripotent stem cells. Stem Cells. 2012;30:797-803. DOI:10.1002/stem.1066
  13. Lombaert IM, Brunsting JF, Wierenga PK, Faber H, Stokman MA, Kok T, Visser WH, Kampinga HH, de Haan G, Coppes RP. Rescue of salivary gland function after stem cell transplantation in irradiated gland. Plos one. 2008;3:e2063. DOI:10.1371/journal.pone.0002063
  14. Andre C, Hampe A, Lachaume P, Martin E, Wang XP, Manus V, Hu WX, Galibert F. Sequence analysis of two genomic regions containing the KIT and the FMS receptor tyrosine kinase genes. Genomics. 1997;37:216-226. DOI:10.1006/geno.1996.4482
  15. Leong KG, Wang BE, Johnson L, Gao WQ. Generation of a prostate from a single adult stem cell. Nature. ;2008;456:804-808. DOI:10.1038/nature07427
  16. Edling CE, Hallberg B. c-Kit a hematopoietic cell essential receptor tyrosine kinase. Int. J. Biochem. Cell Biol. 2007;39:1995-1998. DOI:10.1016/j.biocel.2006.12.005
  17. Sullivan CA, Haddad RI, Tishler RB, Mahadevan A, Krane JF. Chemoradiation-Induced Cell Loss in Human Submandibular Glands. The Laryngoscope. 2005;115:958-964. DOI:10.1097/01.MLG.0000163340.90211.87
  18. Rota M, LeCapitaine N, Hosoda T, Boni A, De Angelis A, Padin-Iruegas ME, et al. Diabetes promotes cardiac stem cell aging and heart failure, which are prevented by deletion of the p66shc gene. Circulation research. 2006;99:42-52. DOI:10.1161/01.RES.0000231289.63468.08
  19. Zhang WJ, Tan YF, Yue JTY, Vranic, Wojtowicz JM. Impairment of hippocampal neurogenesis in streptozotocintreated diabetic rats. Acta Neurologica Scandinavica. 2008;117:205-210. DOI:10.1111/j.1600-0404.2007.00928.x
  20. Beauquis J, Saravia F, Coulaud J, Roig P, Dardenne M, Homo-Delarche F, De Nicola A. Prominently decreased hippocampal neurogenesis in a spontaneous model of type 1 diabetes, the nonobese diabetic mouse. Experimental neurology. 2008;210:359-367. DOI:10.1016/j.expneurol. 2007.11.009