Journal of the Korean Magnetic Resonance Society (한국자기공명학회논문지)

Korean Magnetic Resonance Society (한국자기공명학회)
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NMR Study on the Preferential Binding of the Zα Domain of Human ADAR1 to CG-repeat DNA Duplex

  • Lee, Ae-Ree (Department of Chemistry and RINS, Gyeongsang National University) ;
  • Choi, Seo-Ree (Department of Chemistry and RINS, Gyeongsang National University) ;
  • Seo, Yeo-Jin (Department of Chemistry and RINS, Gyeongsang National University) ;
  • Lee, Joon-Hwa (Department of Chemistry and RINS, Gyeongsang National University)
  • Received : 2017.07.24
  • Accepted : 2017.08.05
  • Published : 2017.09.20
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Abstract

The Z-DNA domain of human ADAR1 ($Z{\alpha}_{ADAR1}$) produces B-Z junction DNA through preferential binding to the CG-repeat segment and destabilizing the neighboring AT-rich region. However, this study could not answer the question of how many base-pairs in AT-rich region are destabilized by binding of $Z{\alpha}_{ADAR1}$. Thus, we have performed NMR experiments of $Z{\alpha}_{ADAR1}$ to the longer DNA duplex containing an 8-base-paired (8-bp) CG-repeat segment and a 12-bp AT-rich region. This study revealed that $Z{\alpha}_{ADAR1}$ preferentially binds to the CG-repeat segment rather than AT-rich region in a long DNA and then destabilizes at least 6 base-pairs in the neighboring AT-rich region for efficient B-Z transition of the CG-repeat segment.

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