Journal of Biomedical Engineering Research (대한의용생체공학회:의공학회지)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
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The Effect of Microcurrent Electrical Stimulation on Muscle Atrophy Suppression in a Sciatic Nerve Injured Rat Model; Comparative Study by Current Intensity

좌골신경손상 쥐 모델을 이용한 미세전류 자극의 근위축 억제 효과 확인 및 자극 세기 별 비교

  • Hwang, Donghyun (Department of Biomedical Engineering, Yonsei University) ;
  • Kim, Seohyun (Department of Biomedical Engineering, Yonsei University) ;
  • Lee, Hana (Department of Biomedical Engineering, Yonsei University) ;
  • Jang, Seungjun (Department of Biomedical Engineering, Yonsei University) ;
  • Kim, Sebin (Department of Biomedical Engineering, Yonsei University) ;
  • kim, Tackjoong (Division of biological science and technology) ;
  • Choi, Sooim (YD Life science company) ;
  • Kwak, Hoyoung (YD Life science company) ;
  • Kim, Han Sung (Department of Biomedical Engineering, Yonsei University)
  • Received : 2017.06.26
  • Accepted : 2017.06.29
  • Published : 2017.08.31
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Abstract

Microcurrent electrical stimulation(MES) has been used to accelerate recovery of atrophied skeletal muscle. However, convincing stimulation parameters for suppressing muscle atrophy due to injured sciatic nerve remains unclear. The objective of this study was to investigate the effective intensity of MES on restraining muscle atrophy with rat model underwent sciatic nerve injury(SNI). Twenty-5-week-old Sprague Dawley male rats were equally assigned to five groups : Control group(Control, CON, n = 4), Denervation group(Denervation, D, n = 4), Denervation with MES of $22{\mu}A$ group(Denervation + $22{\mu}A$, D+22, n = 4), Denervation with MES of $100{\mu}A$ group (Denervation + $100{\mu}A$, D+100 n = 4), Denervation with MES of $400{\mu}A$ group(Denervation + $400{\mu}A$, D+400, n = 4). To induce muscle atrophy, all rats in the D, D+22, D+100, and D+400 groups, were subjected to sciatic nerve injury on their right hindlimb and allowed to have 1 week of resting period. Following this period, rats underwent daily MES(60 min/ a day, 5times/1week) for 4 weeks. After that, we investigate morphological changes in muscle volume by using in vivo micro-computed tomography at week 0, 1, 3 and 5. After 5 weeks, the muscle volume had the highest value in D+400 group, and also noticeably increased in D+100 group compared to it in D group. The results of this study imply that MES with current intensities between $100-400{\mu}A$ can suppress muscle atrophy effectively.

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References

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