KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Whitening Activities of the Halophyte L. tetragonum (Thunberg) A. A. Bullock Extract in B16F10 Melanoma Cells

염생식물 갯질경 추출물의 미백 활성

  • Kim, Min-Jin (Nakdonggang National Institute of Biological Resources) ;
  • Hyun, Kwang Hee (Helios Co., Ltd.) ;
  • Kim, Ji-Hye (Helios Co., Ltd.) ;
  • Han, Dong Hwan (Department of BT Convergent Pharmaceutical Engineering, Sunmoon University) ;
  • Kim, Seung-Young (Department of BT Convergent Pharmaceutical Engineering, Sunmoon University) ;
  • Lee, Nam Ho (Department of Chemistry and Cosmetics, Jeju National University) ;
  • Hyun, Chang-Gu (Department of Chemistry and Cosmetics, Jeju National University)
  • Received : 2017.08.03
  • Accepted : 2017.08.24
  • Published : 2017.09.30
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Abstract

To identify the natural and novel cosmeceutical ingredients which have whitening function, we examined the effects of tyrosinase activity and melanin production on halophyte Limonium tetragonum (Thunb.) A. A. Bullock extracts. As a result, the extracts showed whitening effect with no cytotoxicity. Melanin contents of B16F10 melanoma cells were decreased in a dose-dependent manner at 50, 100, $200{\mu}g/mL$ treatment of the extracts. In tyrosinase activity inhibition test, L. tetragonum (Thunb.) A. A. Bullock extracts showed decreased tyrosinase activity as the concentration of ${\alpha}-MSH$ was increased. Furthermore, it was observed that the tyrosinase and MITF expression was significantly downregulated by adding L. tetragonum (Thunb.) A. A. Bullock extracts in ${\alpha}-MSH$, a melanogenesis inducing material, treated B16F10 melanoma cells. These results indicate that L. tetragonum (Thunb.) A. A. Bullock extract might be an effective whitening agent by inhibit melanin formation.

Keywords

Acknowledgement

Supported by : 한국연구재단

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