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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Solid State Fermentation of Phanerochaete chrysosporium for Degradation and Saccharification of Lignocellulose

Phanerochaete chrysosporium의 고상발효를 통한 리그노셀룰로오즈 분해 및 당화

  • Utomo, Romualdus N.C. (Department of Biological Engineering, Inha University) ;
  • Lee, Eun-Kwang (Department of Biological Engineering, Inha University) ;
  • Yun, Hyun Shik (Department of Biological Engineering, Inha University)
  • Received : 2017.03.27
  • Accepted : 2017.05.15
  • Published : 2017.06.30
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Abstract

The lignocellulose that is a major component of spent coffee ground was degraded and saccharified. To implement the spent coffee, after several pre-treatments, inoculation of Phanerochaete chrysosporium and solid-state fermentation were conducted. The optimal temperature of the enzymes (lignin peroxidase, manganese peroxidase, xylanase, laccase, and cellulase) for degradation of lignocellulose by P. chrysosporium was found. We also measured the maximum activity of enzymes (lignin peroxidase 0.15 IU/mL, manganese peroxidase 0.90 IU/mL, laccase 0.11 IU/mL, cellulase 5.87 IU/mL, carboxymethyl cellulase 9.52 IU/mL, xylanase 1.16 IU/mL) used for the process. As a result, 4.73 mg/mL of reduced sugar was obtained and 61.02% of lignin was degraded by solid state fermentation of P. chrysosporium on spent coffee ground.

Keywords

References

  1. Kondamudi, N., S. K. Mohapatra, and M. Misra (2008) Spent coffee grounds as a versatile source of green energy. J. Agric. Food Chem. 5624: 11757-11760.
  2. Zhang, H. Mo. and H. Nam (2011) Bioconversion of cellulose to bioalcohol. Proceedings of 2011 International Conference on Agricultural and Biosystems Engineering (ICABE 2011), February 20, Hong Kong, China.
  3. Hahn-Hagerdal, B., M. Galbe, M.-F. Gorwa-Grauslund, G. Liden, and G. Zacchi (2006) Bio-ethanol the fuel of tomorrow from the residues of today. Trends Biotechnol. 2412: 549-556.
  4. Srivastava, A. K. and P. Agrawal (2012) Saccharification with Phanerochaete chrysosporium and ethanol production with Saccharomyces cerevisiae. J. Atoms Molecules. 24: 321.
  5. Ghasemi, F., F. Tabandeh, B. Bambai, and K. S. Rao (2010) Decolorization of different azo dyes by Phanerochaete chrysosporium RP 78 under optimal condition. Int. J. Environ. Sci. Technol. 73: 457-464.
  6. Levasseur, A., E. Drula, V. Lombard, P. M. Coutinho, and B. Henrissat (2013) Expansion of the enzymatic repertoire of the CAZy database to integrate auxiliary redox enzymes. Biotechnol. Biofuels 6: 41-44. https://doi.org/10.1186/1754-6834-6-41
  7. Asgher, M., M. Asad, and R. Legge (2006) Enhanced lignin peroxidase synthesis by Phanerochaete chrysosporium in solid state bioprocessing of a lignocellulosic substrate. World J. Microbiol. Biotechnol. 225: 449-453.
  8. Christian, V., R. Shrivastava, D. Shukla, H. Modi, and B. Vyas (2005) Degradation of xenobiotic compounds by lignin-degrading white-rot fungi: Enzymology and mechanisms involved. Indian. J. Exp. Biol. 43: 301-312.
  9. Popp, J. L., B. Kalyanaraman, and T. K. Kirk (1990) Lignin peroxidase oxidation of manganese (2+) in the presence of veratryl alcohol, malonic or oxalic acid, and oxygen. Biochem. 2946: 10475-10480.
  10. Glenn, J. K., L. Akileswaran, and M. H. Gold (1986) Mn (II) oxidation is the principal function of the extracellular Mn-peroxidase from Phanerochaete chrysosporium. Arch Biochem. Biophys. 2512: 688-696.
  11. Han, M. J., H. T Choi, and H. G Song (2005) Purification and characterization of laccase from the white rot fungus Trametes versicolor. J. Microbiol. 43: 555-560.
  12. Pandit, N. and S. Maheshwari (2012) Optimization of cellulase enzyme production from sugarcane pressmud using oyster mushroom-pleurotus sajor-caju by solid state fermentation. J Bioremed. Biodegrad. 3: 140-144.
  13. Flores, M., R. Perez, and C. Huitron (1997) $\beta$-Xylosidase and xylanase characterization and production by Streptomyces sp. CH-M-1035. Lett. Appl. Microbiol. 245: 410-416.
  14. Tien, M. and T. K. Kirk (1984) Lignin-degrading enzyme from Phanerochaete chrysosporium: Purification, characterization, and catalytic properties of a unique $H_2O_2$-requiring oxygenase. Proc. Natl. Acad. Sci. 818: 2280-2284.
  15. Kumar, A.G., G. Sekaran, and S. Krishnamoorthy (2006) Solid state fermentation of Achras zapota lignocellulose by Phanerochaete chrysosporium. Bioresour. Technol. 9713: 1521-1528.
  16. Lu, L., M. Zhao, B.-B. Zhang, S.-Y. Yu, X.-J. Bian, W. Wang, and Y. Wang (2007) Purification and characterization of laccase from Pycnoporus sanguineus and decolorization of an anthraquinone dye by the enzyme. Appl. Microbiol. Biotechnol. 746: 1232-1239.
  17. Ghose, T. (1987) Measurement of cellulase activities. Pure Appl. Chem. 592: 257-268.
  18. Ghose, T. and V. S. Bisaria (1987) Measurement of hemicellulase activities: Part I Xylanases. Pure Appl. Chem. 5912: 1739-1751.
  19. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 721-2: 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
  20. Sluiter, A., B. Hames, R. Ruiz, C. Scarlata, J. Sluiter, D. Templeton, and D. Crocker (2008) Determination of Structural Carbohydrates and Lignin in Biomass. NREL/TP-510-42618, US National Renewable Engergy Laboratory, Golden Colorado.
  21. Asther, M., C. Capdevila, and G. Corrieu (1988) Control of lignin peroxidase production by Phanerochaete chrysosporium INA-12 by temperature shifting. Appl. Environ. Microbiol. 5412: 3194-3196.
  22. Ryu, W. R., S. H. Shim, M. Y. Jang, Y. J. Jeon, K. K. Oh, and M. H. Cho (2000) Biodegradation of pentachlorophenol by white rot fungi under ligninolytic and nonligninolytic conditions. Biotechnol. Bioprocess Eng. 53: 211-214.
  23. Kanmani, P. and P. Karuppasamy (2009) Studies on lignocellulose biodegradation of coir waste in solid state fermentation using Phanerocheate chrysosporium and Rhizopus stolonifer. Afr. J. Biotechnol. 8: 6880-6887.
  24. Dobozi, M. S., G. Szakacs, and C. V. Bruschi (1992) Xylanase activity of Phanerochaete chrysosporium. Appl. Environ. Microbiol. 5811: 3466-3471.
  25. Liu, Y.-H. (2011) Surfactant enhanced the hydrolysis of lignocellulose using Phanerochaete chrysosporium. Proceedings of 2011 International Conference on Agricultural and Biosystems Engineering (ICABE 2011), February 20, Hong Kong, China.