Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Hypolipidemic and Hypoinsulinemic Effects of Dietary Fiber from Agar in C57BL/6N Mice Fed a High-fat Diet

고지방사료를 섭취한 C57BL/6N 마우스에서 한천식이섬유의 혈청 지질과 인슐린 농도 저해효과

  • Park, Jin Ju (Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Kim, Ji Eun (Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Yun, Woo Bin (Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Lee, Mi Lim (Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Choi, Jun Young (Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Song, Bo Ram (Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Kim, Dong Seob (Department of Foods Science & Technology, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University) ;
  • Lee, Chung Yeoul (Gangrim Organics) ;
  • Lee, Hee Seob (Department of Food Science & Nutrition, College of Human Ecology, Pusan National University) ;
  • Lim, Yong (Department of Clinical Laboratory Science, College of Nursing and Healthcare Science, Dong-Eui University) ;
  • Jung, Min Wook (Research Center, Miryang Agar-Agar Co., LTD) ;
  • Hwan, Dae Youn (Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University)
  • 박진주 (부산대학교 생명자원과학대학 바이오소재과학과 & 생명융합연구센터) ;
  • 김지은 (부산대학교 생명자원과학대학 바이오소재과학과 & 생명융합연구센터) ;
  • 윤우빈 (부산대학교 생명자원과학대학 바이오소재과학과 & 생명융합연구센터) ;
  • 이미림 (부산대학교 생명자원과학대학 바이오소재과학과 & 생명융합연구센터) ;
  • 최준영 (부산대학교 생명자원과학대학 바이오소재과학과 & 생명융합연구센터) ;
  • 송보람 (부산대학교 생명자원과학대학 바이오소재과학과 & 생명융합연구센터) ;
  • 김동섭 (부산대학교 생명자원과학대학 식품공학과) ;
  • 이충열 ((주)강림오가닉) ;
  • 이희섭 (부산대학교 생활환경대학 식품영양학과) ;
  • 임용 (동의대학교 의료보건생활대학 임상병리학과) ;
  • 정민욱 ((주)밀양한천) ;
  • 황대연 (부산대학교 생명자원과학대학 바이오소재과학과 & 생명융합연구센터)
  • Received : 2017.04.30
  • Accepted : 2017.08.17
  • Published : 2017.08.30
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Abstract

To investigate the beneficial effects of fiber derived from agar on the lipid and glucose metabolism of obese model mice, changes in the fat accumulation, lipid content, and insulin concentration were measured in C57BL/6N mice fed a high-fat diet (HFD) and treated with a beverage containing agar (BCA) for 9 weeks. The feed efficiency ratio was significantly decreased in the HFD+BCA treatment group, although the body weight and food intake were maintained a constant level. Also, the fat accumulation in the fatty tissue and liver were lower in the HFD+BCA treatment group than in the HFD+Vehicle treatment group. Furthermore, a significant decrease was detected in the levels of total cholesterol (TC) and LDL after the BCA treatment, while a constant HDL level was maintained in the same group. Moreover, the serum insulin concentration was significantly decreased in the HFD+BCALo (Low concentration) and HFD+BCAMi (Middle concentration) groups compared to the HFD+Vehicle group. Therefore, the results suggest that the long-term administration of dietary fiber from agar can improve fat accumulation in the fatty tissue and liver, the serum lipid profile, and the insulin concentration in the blood. Also, the results provide evidence that the inclusion of agar fiber in beverages results in significant hypolipidemic and hypoinsulinemic effects.

본 연구에서는 한천식이섬유(Diet fiber of agar)가 지방과 포도당대사에 미치는 효과를 연구하고자, 고지방사료(High fat diet, HFD)를 급여한 C57BL/6 마우스에 한천함유음료(beverage contained agar, BCA)를 9주 동안 투여하고 지방축적, 지질농도, 인슐린농도 등의 변화를 분석하였다. 식이섭취효율은 HFD+BCA 처리그룹에서 유의적으로 감소하였지만, 체중과 식이섭취량은 HFD+BCA 처리그룹에서 일정하게 유지되었다. 또한, 지방조직과 간조직에서 지방의 축적은 HFD+Vehicle처리그룹에 비하여 HFD+BCA 처리그룹에서 낮게 관찰되었다. 더불어, BCA 처리후에 유의적인 변화는 total cholesterol (TC)과 LDL에서 관찰되었지만, HDL의 농도는 BCA처리에 의해 변화가 없었다. 인슐린의 농도는 HFD+BCAMi 처리그룹과 비교하여 HFD+BCALo과 HFD+BCAMi 처리그룹에서 유의적으로 감소하였다. 따라서 이러한 결과는 한천식이섬유의 장기간 투여는 고지방사료를 급여한 동물에서 지방축적, 혈청내 지방프로파일, 인슐린농도의 개선을 유도할 수 있음을 제시하고 있다. 또한, 이러한 결과는 한천식이 섬유를 함유하는 음료는 혈청내 지질과 인슐린 작용을 유도할 수 있음을 제시하고 있다.

Keywords

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