생명과학회지 (Journal of Life Science)

  • 제27권8호
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  • Pages.919-929
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  • 2017
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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산지별 홍국발효 참당귀의 이화학적 특성 및 생리활성

Physicochemical Characteristics and Biological Activities of Monascus-fermented Angelica gigas Nakai by Origin

  • 투고 : 2017.05.08
  • 심사 : 2017.07.25
  • 발행 : 2017.08.30
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초록

본 연구는 발효 참당귀를 이용한 건강 기능성 식품개발을 위해 산청, 평창 및 제천산 참당귀를 Monascus purpureus로 발효시켜 제조한 발효 참당귀의 이화학적 특성 및 생리활성을 평가하였다. 발효 참당귀의 decursin 및 decursinol angelate 함량은 발효전보다 증가한 바, 평창 참당귀(PA)군의 615.504 및 326.742 ppm이 발효 후 각각 792.610 및 429.500 ppm으로 증가되었다. 총 페놀성 화합물과 플라보노이드 및 미네랄 함량이 발효로 인해 증가하였고, DPPH를 이용한 자유라디칼 소거능 및 Fe, Cu를 이용한 환원력 비교를 통해 발효 참당귀군에서 높게 나타났으며, 이중 FPA군에서 활성이 높은 것을 확인하였다. LPS처리 RAW 264.7세포의 NO생성능과 유방암 세포(MCF-7) 및 간암 세포(Hep3B) 성장 저해능 확인 결과 발효 참당귀군에서 활성이 높게 나타났다. 이와 같이 M. purpureus 발효 산청, 평창 및 제천 참당귀를 비교한 결과 평창 참당귀에서 건강 기능성 식품으로의 활용 가치가 높을 것으로 사료된다.

This study investigated the biological activities and chemical characteristics of Monascus-fermented Angelica gigas Nakai (A. gigas) with a view to the development of health foods. We also investigated the effect of the cultivation region of three A. gigas cultivars, namely Sancheong, Pyeongchang, and Jecheon. After fermentation, the content of decursin and decursinol angelate was increased compared to non-fermentation; the highest content of decursin and decursinol angelate was found in non-fermentation Pyeongchang A. gigas (PA) at 615.504, 326.742 ppm and fermented Pyeongchang A. gigas (FPA) at 792.610, 429.500 ppm, respectively. The highest content of phenolic compounds, flavonoids, and minerals was found in the FPA group, in which DPPH (${\alpha},{\alpha}^{\prime}-diphenyl-{\beta}-picrylhydrazyl$) radical scavenging activity and Fe/Cu reducing power were stronger in fermented than in non-fermented A. gigas. The FPA group in particular showed the highest activity. We measured nitric oxide (NO) production from lipopolysaccharide-induced RAW 264.7 cells and the inhibition of cancer cell proliferation. The inhibition of activity of NO production and cancer cell (MCF-7 and Hep3B) viability was significantly decreased in the FPA group. The results suggest that FPA may be highly useful as a health food. Overall, the study provides basic data for understanding the biological activities and chemical characteristics of A. gigas fermented by Monascus purpureus for the development of health foods.

키워드

참고문헌

  1. A.O.A.C. 1975. Official methods of analysis. 12th ed., Association of official analytical chemists. Washington, D.C., U.S.A.
  2. Ahn, H. Y., Park, K. R., Kim, Y. R., Yoon, K. H., Kim, J. W. and Cho, Y. S. 2013. Effects of Monascus-fermented Angelica gigas Nakai on the contents of serum lipid and tissue lipid peroxidation in alcohol feeding rats. J. Life Sci. 23, 1371-1380. https://doi.org/10.5352/JLS.2013.23.11.1371
  3. Ahn, M. J., Lee, M. K., Kim, Y. C. and Sung, S. H. 2008. The simultaneous determination of coumarins in Angelica gigas root by high performance liquid chromatography-diode array detector coupled with electrospray ionization/mass spectrometry. J. Pharm. Biomed. Anal. 46, 258-266. https://doi.org/10.1016/j.jpba.2007.09.020
  4. Ancerewicz, J., Miqliavacca, E., Carrupt, P. A., Testa, B., Bree, F., Zini, R., Tillement, J. P., Labidalle, S., Guyot, D., Chauvet-Monges, A. M., Crevat, A. and Le Ridant, A. 1998. Structure-property relationships of trimetazidine derivatives and model compounds as potential antioxidants. Free Radic. Biol. Med. 25, 113-120. https://doi.org/10.1016/S0891-5849(98)00072-0
  5. Baskar, R., Rajeswari, V. and Kumar, T. S. 2007. In vitro antioxidant studies in leaves of Annona species. Indian J. Exp. Biol. 45, 480-485.
  6. Blois, M. S. 1958. Antioxidant determination by the use of a stable free radical. Nature 26, 1199-1204.
  7. Cha, J. Y., Kim, H. J., Chung, C. H. and Cho, Y. S. 1999. Antioxidative activities and contents of polyphenolic compound of Cudrania tricuspidata. J. Kor. Soc. Food Sci. Nutr. 28, 1310-1315.
  8. Cha, J. Y., Park, J. C., Ahn, H. Y., Eom, K. E., Park, B. K., Jun, B. S. and Cho, Y. S. 2009. Effect of Monascus purpureus fermented Korean red ginseng powder on the serum lipid levels and antioxidative activity in rats. J. Kor. Soc. Food Sci. Nutr. 38, 1153-1160. https://doi.org/10.3746/jkfn.2009.38.9.1153
  9. Cho, T. D. 2011. Encyclopedia of Korean Herbs, pp. 59, Korea.
  10. Choi, M. G., Bang, J. K. and Chae, Y. A. 2003. Comparison of volatile compounds in plant parts of Angelica gigas Nakai and A. acutiloba Kitagawa. Kor. J. Med. Crop Sci. 11, 352-357.
  11. Duncan, D. B. 1959. Multiple range and multiple F test. Biometrics 1, 1-42.
  12. Han, E. J., Roh, S. B. and Bae, S. J. 2000. Effects of quinone reductase induction and cytotoxicity of the Angelica radix extracts. J. Kor. Soc. Food Sci. Nutr. 29, 147-152.
  13. Han, S. K. 2005. Quality improvement of effective microorganism (EM) pork produced by using EM. J. Kor. Soc Food Sci Nutr. 34, 734-737. https://doi.org/10.3746/jkfn.2005.34.5.734
  14. Heo, J. S., Cha, J. Y., K, H. W., Ahn, H. Y., Eom, K. E., Heo, S. J. and Cho, Y. S. 2010. Bioactive materials and biological activity in the extracts of leaf, stem mixture and root from Angelica gigas Nakai. J. Life Sci. 20, 760-769. https://doi.org/10.5352/JLS.2010.20.5.760
  15. Hwang, J. and Hseu, T. H. 1980. Specificity of the acid protease from Monascus kaoliang towards the B-chain of oxidized insulin. Biochim Biophys Acta. 614, 607-612. https://doi.org/10.1016/0005-2744(80)90250-8
  16. Jia, Z., Tang, M. and Wu, J. 1999. The determination of flavonoid contents in mulberry and thier scavenging effects on superoxide radicals. Food Chem. 64, 555-559. https://doi.org/10.1016/S0308-8146(98)00102-2
  17. Jin, Y. J. and Pyo, Y. H. 2015. Effect of Monascus-fermentation on the content of bioactive compounds in white and black soybeans. Kor. J. Food Sci. Technol. 47, 409-412. https://doi.org/10.9721/KJFST.2015.47.3.409
  18. Kang, D. Z., Um, J. B., Lee, S. K. and Lee, J. H. 2003. Content of rutin and monacolin K in the red buckwheat fermented with Monascus ruber. Kor. J. Food Sci. Technol. 35, 242-245.
  19. Kang, S. A., Han, J. A., Jang, K. H. and Choue, R. W. 2004. DPPH radical scavenger activity and antioxidant effects of Cham-Dang-Gui (Angelica gigas). J. Kor. Soc Food Sci Nutr. 33, 1112-1118. https://doi.org/10.3746/jkfn.2004.33.7.1112
  20. Kang, Y. Y., Lee, J. H., Chae, H. J., Kim, D. H., Lee, S. H. and Park, S. Y. 2003. HPLC Analysis and extraction methods of decursin and decursinol angelate in Angelica gigas roots. Kor. J. Pharmacogn. 67, 201-205.
  21. Kim, E. Y., Baik, I. H., Kim, J. H., Kim, S. R. and Rhyu, M. R. 2004. Screening of the antioxidant activity of some medicinal plants. Kor. J. Food Sci. Technol. 36, 333-338.
  22. Kim, H. S. and Joung, S. W. 2006. Effective components and nitrite scavenging ability of root and leaves a Angelica gigas Nakai. Kor. J. Food Cookery Sci. 22, 957-965.
  23. Kim, J. Y., Yoon, Y. D., Ahn, J. M., Kang, J. S., Park, S. K., Lee, K., Song, K. B., Kim, H. M. and Han, S. B. 2007. Angelan isolated from Angelica gigas Nakai induces dendritic cell maturation through toll-like receptor 4. Int. Immunopharmacol. 7, 78-87. https://doi.org/10.1016/j.intimp.2006.08.017
  24. Kim, K. M., Jung, J. Y., Hwang, S. W., Kim, M. J. and Kang, J. S. 2009. Isolation and purification of decursin and decursinol angelate in Angelica gigas Nakai. J. Kor. Soc. Food Sci. Nutr. 38, 653-656. https://doi.org/10.3746/jkfn.2009.38.5.653
  25. Kwon, D. H., Kang, H. J., Choi, Y. H., Chung, K. T., Lee, J. H., Kang, K. H., Hyun, S. K., Kim, B. W. and Hwang, H. 2016. Immunomodulatory activity of water extract of Ulmus macrocarpa in Macrophages. J. Life Sci. 26, 50-58. https://doi.org/10.5352/JLS.2016.26.1.50
  26. Kwon, J. H., Han, M. S., Lee, B. M. and Lee, Y. M. 2015. Effect of Angelica gigas extract powder on progress of osteoarthritis induced by monosodium iodoacetate in rats. Anal. Sci. Technol. 28, 72-77. https://doi.org/10.5806/AST.2015.28.1.72
  27. Lee, C. Y., Kim, K. M. and Son, H. S. 2013. Optimal extraction conditions to produce rosemary extracts with higher phenolic content and antioxidant activity. Kor. J. Food Sci. Technol. 45, 501-507. https://doi.org/10.9721/KJFST.2013.45.4.501
  28. Lee, J. H., Kwak, E. J., Kim, J. S., Lee, K. S. and Lee, Y. S. 2007. A study on quality characteristics of sourdough breads with addition of red yeast rice. J. Kor. Soc. Food Sci Nutr. 36, 785-793. https://doi.org/10.3746/jkfn.2007.36.6.785
  29. Lee, S. G. and Kim, M. M. 2015. Anti-inflammatory effect of scopoletin in RAW264.7 Macrophages. J. Life Sci. 25, 1377-1383. https://doi.org/10.5352/JLS.2015.25.12.1377
  30. Lee, S. Y. and Lee, J. Y. 2013. Inhibitory efficacy of Angelica gigas Nakai on microphthalmia-associated transcription factor (MITF), tyrosinase related Protein (TRP-1), tyrosinase related protein-2 (TRP-2), and tyrosinase mRNA expression in melanoma cells (B16F10). J. Life Sci. 23, 1336-1341. https://doi.org/10.5352/JLS.2013.23.11.1336
  31. Park, K. W., Choi, S. R., Shon, M. Y., Jeong, I. Y., Kang, K. S., Lee, S. T., Shim, K. H. and Seo, K. I. 2007. Cytotoxic effects of decursin from Angelica gigas Nakai in human cancer cells. J. Kor. Soc. Food Sci. Nutr. 36, 1385-1390. https://doi.org/10.3746/jkfn.2007.36.11.1385
  32. Park, K. Y. 2012. Increased health functionality of fermented foods. Food Industry and Nutrition 17, 1-8.
  33. Ryu, K. S., Hong, N. D. and Kim, Y. Y. 1990. Studies on the coumarin constituents of the root of Angelica gigas Nakai. Isolation of decursinol angelate and assay of decursinol angelate and decursin. Kor. J. Pharmacogn. 21, 64-68.
  34. Sa, Y. J., Kim, J. S., Kim, M. O., Jeong, H. J., Yu, C. Y., Park, D. S. and Kim, M. J. 2010. Comparative study of electron donating ability, reducing power, antimicrobial activity and inhibition of ${\alpha}$-glucosidase by Sorghum bicolor extracts. Kor. J. Food Sci. Technol. 42, 598-604.
  35. Seong, N. S., Lee. S. W., Kim, K. S. and Lee, S. T. 1993. Environmental variation of decursin content in Angelica gigas. Kor. J. Crop Sci. 38, 60-65.
  36. Swain, T., Hillis, W. E. and Oritega, M. 1959. Phenolic constituents of Ptunus domestica. I. Quantitative analysis of phenolic constituents. J. Sci. Food Agriculture 10, 63-68. https://doi.org/10.1002/jsfa.2740100110
  37. Sweeny, J. G., Estrada-Valdes, M. C., Lacobucci, G. A., Sato, H. and Sakamura, S. 1981. Photoprotection of the red pigments of Monascus anka in aqueous media by 1,4,6-trihydroxynaphthalene. J. Agric. Food. Chem. 29, 1189-1193. https://doi.org/10.1021/jf00108a023
  38. Zhu, Q. V., Hackman, R. M., Jodilensunsa, X. X., Holt, R. R. and Keen, C. L. 2002. Antioxidative activities of Oolong tea. J. Agric. Food Chem. 50, 6229-6234.