Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Inhibition Effect of Phenolic Compounds from Ultra-fine Ground Chrysanthemum indicum L. on Xanthine Oxidase

초미세 분쇄한 감국으로부터 추출된 phenolic 화합물의 xanthine oxidase 저해 효과

  • Cho, Young-Je (School of Food science & Biotechnology / Food & Bio-Industry Research Institute, Kyungpook National University) ;
  • Kim, Byung-Oh (School of Food science & Biotechnology / Food & Bio-Industry Research Institute, Kyungpook National University) ;
  • Park, Hye-Jin (School of Food science & Biotechnology / Food & Bio-Industry Research Institute, Kyungpook National University) ;
  • Lee, Eun-Ho (School of Food science & Biotechnology / Food & Bio-Industry Research Institute, Kyungpook National University) ;
  • Jo, Jae-Bum (School of Food science & Biotechnology / Food & Bio-Industry Research Institute, Kyungpook National University) ;
  • Lee, Jae-Eun (School of Food science & Biotechnology / Food & Bio-Industry Research Institute, Kyungpook National University) ;
  • Lim, Su-Bin (School of Food science & Biotechnology / Food & Bio-Industry Research Institute, Kyungpook National University) ;
  • Kim, Ye-Jin (School of Food science & Biotechnology / Food & Bio-Industry Research Institute, Kyungpook National University) ;
  • Park, Ki-Tae (School of Culinary Art and Baking technology, Dongju College University) ;
  • Choi, Moo-Young (Department of Food and Nutrition, College of Health Science, Sangji University)
  • 조영제 (경북대학교 식품공학부/식품생물산업연구소) ;
  • 김병오 (경북대학교 식품공학부/식품생물산업연구소) ;
  • 박혜진 (경북대학교 식품공학부/식품생물산업연구소) ;
  • 이은호 (경북대학교 식품공학부/식품생물산업연구소) ;
  • 조재범 (경북대학교 식품공학부/식품생물산업연구소) ;
  • 이재은 (경북대학교 식품공학부/식품생물산업연구소) ;
  • 임수빈 (경북대학교 식품공학부/식품생물산업연구소) ;
  • 김예진 (경북대학교 식품공학부/식품생물산업연구소) ;
  • 박기태 (동주대학교 외식조리제과계열) ;
  • 최무영 (상지대학교 식품영양학과)
  • Received : 2017.04.04
  • Accepted : 2017.06.19
  • Published : 2017.08.30
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Abstract

In this study, the extracted phenolic compounds from 98 species of oriental herbal medicine were examined for biological activities to be used as functional resources. In particular, the anti-gout effect by xanthine oxidase (XOase) inhibition was determined using water and ethanol as extraction solvents because of their non-toxicity in the human body. The extracts of Chrysanthemum indicum L. (83.45%), Cuscuta chinensis (60.22%), Asiasarum sieboldi F. Maekawa (51.66%), Acorus gramineus (67.8%), Aconitum pseudo-laeve var. erectum (75.23%), Thuja orientalis (47.27%), Polygonum aviculare (53.98%), Carthami semen (63.99%), and Syzygium aromaticum (40.22%) showed relatively high XOase inhibitory activity. Chrysanthemum indicum L. was selected for its high XOase inhibitory activity. The biological compounds in Chrysanthemum indicum L. were identified to contain phenolics included in extracts of solids. Ultra-fine grind technology showed a higher extraction yield than normal grind and fine grind technology. Ethanol extracts showed relatively higher XOase inhibitory activity than water extracts. XOase inhibitory activity increased in a dependent manner as phenolic concentration increased. Therefore, ultra-fine grind technology was confirmed for use in increasing the extraction yield of XOase inhibitory compounds from Chrysanthemum indicum L.. Extracts from Chrysanthemum indicum L. are expected to be a useful functional resource for the prevention or treatment of gout.

본 연구에서 98종의 한약재로부터 인체에 무해한 물과 ethanol을 용매로 이용한 추출물에 대하여 xanthine oxidase(XOase)의 저해에 의한 통풍억제 효과를 측정한 결과 Chrysanthemum indicum L. (83.45%), Cuscuta chinensis (60.22%), Asiasarum sieboldi F. Maekawa (51.66%), Acorus gramineus (67.8%), Aconitum pseudo-laeve var. erectum (75.23%), Thuja orientalis (47.27%), Polygonum aviculare (53.98%), Carthami semen (63.99%), Syzygium aromaticum (40.22%) 등이 비교적 높은 XOase 저해율을 나타내었다. 감국의 경우 물과 ethanol 추출물 모두에서 XOase 저해활성이 가장 높게 측정되어 시료로 선발되었다. 감국의 유효성분을 확인한 결과 단순 고형분보다 고형분에 포함된 phenolic 성분이 XOase 저해 효과에 관여함이 입증되었다. 효율적인 추출을 위한 분쇄조건은 일반분쇄보다는 초미세 분쇄한 시료에서 추출 수율이 높아짐이 확인되었다. 추출된 감국 추출물의 XOase 저해 활성은 ethanol 추출물이 물 추출물에 비해 상대적으로 높은 저해 활성을 나타내었으며, phenolics의 첨가농도가 높아지면서 XOase에 대한 저해 활성은 농도 의존적으로 증가하는 것으로 확인되었다. 따라서 초미세 분쇄 기술은 감국으로부터 XOase 저해 물질의 추출 수율 증가를 목적으로 적용할 수 있는 기술로 개발이 가능할 것으로 판단되었다.

Keywords

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