Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Development of Atopic Dermatitis Mouse Model with Spleen Deficiency

비허형 아토피 동물모델 개발

  • Yang, Won Kyung (Division of Respiratory System, Department of Internal Medicine, College of Korean Medicine, Daejeon University) ;
  • Lyu, Yee Ran (Division of Respiratory System, Department of Internal Medicine, College of Korean Medicine, Daejeon University) ;
  • Kim, Ho Kyoung (Korea Institute of Oriental Medicine) ;
  • Kim, Seung Hyeong (Division of Respiratory System, Department of Internal Medicine, College of Korean Medicine, Daejeon University) ;
  • Park, Yang Chun (Division of Respiratory System, Department of Internal Medicine, College of Korean Medicine, Daejeon University)
  • 양원경 (대전대학교 한의과대학 폐계내과학교실) ;
  • 유이란 (대전대학교 한의과대학 폐계내과학교실) ;
  • 김호경 (한국한의학연구원) ;
  • 김승형 (대전대학교 한의과대학 폐계내과학교실) ;
  • 박양춘 (대전대학교 한의과대학 폐계내과학교실)
  • Received : 2017.07.14
  • Accepted : 2017.08.23
  • Published : 2017.08.25
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Abstract

Atopic dermatitis (AD) is a common skin disease characterized by chronic and relapsing inflammatory dermatitis with immunological disturbances. Spleen deficiency (脾虛) is one of the major causes of AD, so development of animal model is required for AD research that reflects the pattern identification. The groups that we have used in this study included Senna folium extracts (SFE), 2,4-dinitrochlorobenzene (DNCB), and normal mice. Therefore, the present study was developed to atopic dermatitis mouse model with spleen deficiency in 2,4-dinitrochlorobenzene (DNCB) and senna leaves extracts induced AD in NC/Nga mice. The results demonstrated that senna leaves extract treatment significantly increased the dermatitis clinical score and epidermal thickness in AD-like skin lesions. We also proved beyond doubt that there was occurrence of erythema and skin moisture indices in the senna leaves extract groups. Further, we also found that the level of serum immunoglobulin E (IgE) in the senna leaves extract-treated group was increased. The amount of IL-4, IL-13, $TNF-{\alpha}$ and $TGF-{\beta}$ mRNA determined by real-time PCR was increased remarkably when senna leaves extract groups were treated on dorsal skin. Senna leaves extract groups significantly promoted the number of CD11B+/Gr-1 cell in skin, as well as the number of CD4+/CD8+ cell in dorsal skin compared with control. The review summarizes recent process in our understanding of the immunopathophysiology of spleen deficiency AD and the implications for spleen deficiency mouse models of AD on drug discovery from medical plants.

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References

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