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Molecular Identification of a Sea Anemone (Cnidaria: Anthozoa: Actiniaria) Obtained in Gijang, Busan

부산 기장에서 채집된 말미잘의 분자생물학적 방법을 이용한 동정

  • Yoo, Sang Joon (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Kim, Do-Hyung (Department of Aquatic Life Medicine, Pukyong National University)
  • 유상준 (부경대학교 수산생명의학과) ;
  • 김도형 (부경대학교 수산생명의학과)
  • Received : 2017.06.07
  • Accepted : 2017.07.27
  • Published : 2017.08.31

Abstract

In this study, we tried to identify a sea anemone collected from the coast of Gijang, Busan. The anemone was morphologically similar to species belonging to the genus Anthopleura, but its morphological characteristics did not allow for confirmed identification to species level. Multiple genes from mitochondrial cytochrome oxidase III, 12S and 16S rRNA, and nuclear 18S and 28S rRNA, were amplified for multilocus sequence typing (MLST) analysis using genomic DNA extracted from the sampled anemone and a different primer set. Based on the MLST analysis, the anemone obtained in this study was identified as Anthopleura artemisia. Also, the sequence of internal transcribed spacer-2 was most closely related to A. artemisia, indicating that this single region might be useful for anemone identification. This study shows significance of molecular identification for sea anemones, and will be helpful in studies of sea anemone identification using genotyping-by-sequencing.

Keywords

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