Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Fast Systemic Evaluation of Amylose and Protein Contents in Collected Rice Landraces Germplasm Using Near-Infrared Reflectance Spectroscopy (NIRS)

근적외선 분광분석기를 이용한 국내외 재래종 벼 유전자원의 아밀로스 및 단백질에 관한 대량 평가 체계구축

  • Oh, Sejong (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) ;
  • Lee, Myung Chul (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) ;
  • Choi, Yu Mi (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) ;
  • Lee, Sukyeung (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) ;
  • Rauf, Muhammad (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) ;
  • Chae, Byungsoo (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) ;
  • Hyun, Do Yoon (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA)
  • 오세종 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 이명철 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 최유미 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 이수경 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • ;
  • 채병수 (농촌진흥청 국립농업과학원 농업유전자원센터) ;
  • 현도윤 (농촌진흥청 국립농업과학원 농업유전자원센터)
  • Received : 2017.04.19
  • Accepted : 2017.08.17
  • Published : 2017.08.31
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Abstract

This study was conducted to characterize the amylose and protein contents of 4,948 rice landrace germplasm using the NIRS model developed in the previous study. The average amylose content of the germplasm was 20.39% and ranged between 3.97 and 37.13%. The amylose contents in the standard rice were 4.99, 18.63 and 20.55% in Sinseonchal, Chucheong and Goami, respectively. The average protein content was 8.17% and ranged from 5.20 to 17.45%. Protein contents in Sinseonchal, Chucheong and Goami were 6.824, 6.869 and 7.839%, respectively. A total of 62% germplasm were distributed between 20.06% and 27.02% in amylose content. Germplasm of 81.60% represented protein content of 6.78-9.75%. The distinguishable ranges of amylose contents according to origin were 16.58-20.06% in Korea, 20.06-23.25% in Japan, 23.25-27.02% in North Korea, and 27.02-37.13% in China. In the protein content, approximately 30% of Chinese resources ranged from 9.75 to 17.45%, whereas less than 10% were detected in other origin accessions. Fifty resources were selected with low and high amylose ranging from 3.97-6.66% and 30.41-37.13%, respectively. Similarly, fifty resources were selected with low and high protein ranging from 5.20-6.09% and 13.21-17.45%, respectively. Landraces with higher protein could be adapted to practical utilization of food sources.

본 연구는 선행연구에서 개발된 근적외선 분광분석(NIRS) 예측모델을 기반으로 국내외 재래종 벼 유전자원의 아밀로스 및 단백질 함량 분석을 실시하여 자원의 분포와 지리적 특성에 대한 정확한 정보를 제공하고 벼 유전자원에 대한 대량평가 체계를 구축하기 위해 수행하였다. 벼 성분분석 표준품의 아밀로스와 NIR 측정값의 비교는 신선찰(성분분석값: 6.881%) 4.994%, 추청(19.731%) 18.633%, 고아미(23.246%) 20.548%였고, 단백질의 경우 신선찰(성분분석값: 6.890%) 6.824%, 추청(6.350%) 6.869%, 고아미(6.777%) 7.839%였다. NIR 측정값은 표준품 성분분석값 대비 아밀로스는 1.1-2.7%포인트 낮았으며 단백질은 0.4-0.6%포인트 높았다. 국내외 재래종 벼 4,948자원의 NIR 스펙트럼은 다양한 작용기들의 결합진동과 배음진동이 나타나는 1,300-2,500 nm에서 큰 흡광도 차이를 보였고, NIRS를 이용한 아밀로스 함량은 3.97-37.13%였으며, 평균 함량은 20.39%였다. 아밀로스 함량 20.06-27.02% 구간에 있는 자원들은 전체 자원의 62.20%를 차지하였다. 단백질 함량구간은 5.20-17.45%였고, 평균 함량은 8.17%였다. 단백질 함량 6.78-9.75% 구간에 있는 자원들은 전체 자원의 81.60%를 차지하였다. 아밀로스 함량과 단백질 함량간에는 유의성이 없었으며 RSQ 값은 0.0012였다. 아밀로스 함량 기준으로 선별된 저아밀로스 50자원은 3.97-6.66%였고 고아밀로스 50자원은 30.41-37.13%였다. 단백질 함량 기준으로 선별된 저단백질 50자원은 5.20-6.09%였고 고아밀로스 50자원은 13.21-17.45%였다. 41개국에서 수집된 재래종 4,948자원 중 한국 재래 자원이 가장 많았으며 24.9%를 차지하였다. 자원수가 가장 많은 아밀로스 함량 구간은 한국 자원이 16.58-20.06%, 일본 자원이 20.06-23.25%, 북한 자원이 23.25-27.02%, 중국 자원이 27.02-37.13%였다. 단백질은 5.20-17.45% 구간에서 전반적으로 유사한 분포를 보였으나 중국 자원은 6.78-8.27% 구간에서 자원비율이 낮았고 9.75-17.45% 구간에서 자원비율이 높았다. 단백질 함량이 높은 자원은 식품적 활용 가치가 높을 것으로 사료된다.

Keywords

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