한국환경과학회지 (Journal of Environmental Science International)

  • 제26권8호
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  • Pages.903-911
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  • 2017
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  • 1225-4517(pISSN)
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  • 2287-3503(eISSN)
한국환경과학회 (The Korean Environmental Sciences Society)
권호 표지
DOI QR코드

DOI QR Code

치자(Gardenia jasminoides Ellis) 과피의 용매별 추출물의 Flavonoid 함량 및 항산화 활성 비교

Comparison of Flavonoid Content and Antioxidant Activities of Peel Extracts from Gardenia jasminoides Ellis by Various Solvents

  • 진동혁 (부산대학교 식품공학과) ;
  • 오다영 (부산대학교 식품공학과) ;
  • 이영근 (부산대학교 식품공학과) ;
  • 강동수 (전남대학교 해양바이오식품학과) ;
  • 김한수 (부산대학교 식품공학과)
  • Jin, Dong-Hyeok (Department of Food Science and Technology, Pusan National University) ;
  • Oh, Da-Young (Department of Food Science and Technology, Pusan National University) ;
  • Lee, Young-Guen (Department of Food Science and Technology, Pusan National University) ;
  • Kang, Dong-Soo (Department of Marine Bio Food Science, Chonnam National University) ;
  • Kim, Han-Soo (Department of Food Science and Technology, Pusan National University)
  • 투고 : 2017.05.11
  • 심사 : 2017.07.27
  • 발행 : 2017.08.31
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초록

The purpose of this study was to measure the bioactivity and antioxidant activity of peel from Gardenia jasminoides fructus Ellis (GJE) in Namhae, Korea, following some established methods. CM (Chloroform:Methanol, 2:1, v/v), 70% ethanol, and n-butanol extracts were collected. Flavonoid content and value as a functional food ingredient of GJE peel was investigated through assessing antioxidant [DPPH (1,1'-diphenyl-2-picrylhydrazyl), ABTS (2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid)], and hydroxyl radical scavenging activities; superoxide dismutase like ability; ferrous ion-chelating capacity; and tannin content by solvent extraction. Solvent extract antioxidant activities significantly increased (p<0.05) at increasing concentrations (0.2, 0.4, 0.6 mg/mL). GJE peel extracts were less active than the positive control [ascorbic acid, BHA (butylated hydroxyanisole), and EDTA (ethylenediaminetetraacetic acid disodium salt dihydrate)]. Based on the results of this study, GJE peel could be used as a natural antioxidant source due to its high antioxidant activity and bioactive compound content.

키워드

참고문헌

  1. Aebi, H., 1984, Catalase in vitro, Methods enzymol., 105, 121-126.
  2. Bagchi, D., Garg, A., Krohn, R. L., Bagchi, M., Tran, M. X., Stohs, S. J., 1997, Oxygen free radical scavenging abilities of vitamins C and E, and a grape seed proanthocyanidin extract in vitro, Res. Commun. Mol. Pathol. Pharmacol., 95, 179-189.
  3. Blois, M. S., 1958, Antioxidant determinations by the use of a stable free radical, Nature, 181, 1199-1200. https://doi.org/10.1038/1811199a0
  4. Chu, Y. E., Chang, C. L., Hsu, H. F., 2000, Flavonoid content of several vegetables and their antioxidant activity, J. Sci. Food Agric., 80, 561-566. https://doi.org/10.1002/(SICI)1097-0010(200004)80:5<561::AID-JSFA574>3.0.CO;2-#
  5. Droge, W., 2002, Free radicals in the physiological control of cell function, Physiol. Rev., 82, 47-95. https://doi.org/10.1152/physrev.00018.2001
  6. Edeoga, H. O., Okwu, D. E., Mbaebie, B. O., 2005, Phytochemical constituents of some Nigerian medicinal plants, African J. Biotechnol., 4, 685-688. https://doi.org/10.5897/AJB2005.000-3127
  7. Floegel, A., Kim, D. O., Chung, S. J., Koo, S. I., Chun, O. K., 2011, Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods, J. Food Compos. Anal., 24, 1043-1048. https://doi.org/10.1016/j.jfca.2011.01.008
  8. Hagerman, A. E., Robbins, C. T., Weerasuriya, Y., Wilson, T. C., Mc Arthur, C., 1992, Tannin chemistry in relation to digestion, J. Range Manage., 45, 57-62. https://doi.org/10.2307/4002526
  9. Heim, K. E., Tagliaferro, A. R., Bobilya, D. J., 2002, Flavonoid antioxidants: Chemistry, metabolism and structure-activity relationships, J. Nutr. Biochem., 13, 572-584. https://doi.org/10.1016/S0955-2863(02)00208-5
  10. Heinonen, M., 2007, Antioxidant activity and antimicrobial effect of berry phenolics A Finnish perspective, Mol. Nutr. Food Res., 51, 684-691. https://doi.org/10.1002/mnfr.200700006
  11. Hertog, M. H., Hollman, P. C., Katan, M. B., 1992, Content of potentially anticarcinogenic flavonoids of 28 vegetables and 9 fruits commonly consumed in the Netherlands, J. Agric. Food Chem., 40, 2379-2383. https://doi.org/10.1021/jf00024a011
  12. Jackson, F. S., Barry, T. N., Lascano, C., Palmer, B., 1996, The extractable and bound condensed tannin content of leaves from tropical tree, shrub and forage legumes, J. Sci. Food Agric., 71, 103-110. https://doi.org/10.1002/(SICI)1097-0010(199605)71:1<103::AID-JSFA554>3.0.CO;2-8
  13. Jin, D. H., Kim, H. S., Seong, J. H., Chung, H. S., 2016, Comparison of total phenol, flavonoid contents, and antioxidant activities of Orostachs japonicus A. Berger extracts, J. Environ. Sci. Int., 25, 695-703. https://doi.org/10.5322/JESI.2016.25.5.695
  14. Kim, H. S., Kim, M. A., Jang, S. H., 2014, Influences of Korean haw (Crataegus pinnatifida BUNGE) on lipid concentration in hypercholesterolemia, J. Environ. Sci. Int., 23, 793-800. https://doi.org/10.5322/JESI.2014.5.793
  15. Koo, S. T., Cho, M. S., Park, S. S., Kim, Y. T., Park, K. J., Kim, K. S., Sohn, I. C., 2005, Effect of frutus gardeniae herbal acupuncture on the rat model of ankle sprain pain, Kor. J. Acupunct., 22, 57-74.
  16. Kostyuk, V. A., Potapovich, A. I., Strigunova, E. N., Kostyuk, T. V., Afanas'ev, I. B., 2004, Experimental evidence that flavonoid metal complexes may act as mimics of superoxide dismutase, Arch. Biochem. Biophys., 428, 204-208. https://doi.org/10.1016/j.abb.2004.06.008
  17. Lee, J. H., Lee, S. R., 1994, Analysis of phenolic substances content in Korean plant foods, Kor. J. Food Sci. Technol., 26, 310-316.
  18. Li, X., 2013, Solvent effects and improvements in the deoxyribose degradation assay for hydroxyl radical-scavenging, Food Chem., 141, 2083-2088. https://doi.org/10.1016/j.foodchem.2013.05.084
  19. McCord, J. M., Fridovich, I., 1969, Superoxide dismutase an enzymic function for erythrocuprein (hemocuprein), J. Biol. Chem., 244, 6049-6055.
  20. Meda, A., Lamien, C. E., Romito, M., Millogo, J., Nacoulma, O. G., 2005, Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity, Food Chem., 91, 571-577. https://doi.org/10.1016/j.foodchem.2004.10.006
  21. Miller, N. J., Rice-Evans, C., Davies, M. J., Gopinathan, V., Milner, A., 1993, A Novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates, Clin. Sci., 84, 407-412. https://doi.org/10.1042/cs0840407
  22. Ming, J., Yaxin, C., Jinrong, L., Hui, Z., 1996, 1, 10 - Phenanthroline $Fe^{2+}$ oxidative assay of hydroxyl radical produced by $H_2O_2$/$Fe^{2+}$, Prog. Biochem. Biophys., 23, 553-555.
  23. Mira, L., Tereza Fernandez, M., Santos, M., Rocha, R., Helena Florêncio, M., Jennings, K. R., 2002, Interactions of flavonoids with iron and copper ions: A Mechanism for their antioxidant activity, Free Radical Res., 36, 1199-1208. https://doi.org/10.1080/1071576021000016463
  24. Robu, S., Aprotosoaie, A., Miron, A., Cioanca, O., Stanescu, U., Hancianu, M., 2012, In vitro antioxidant activity of ethanolic extracts from some Lavandula species cultivated in Romania, Farmacia, 60, 394-401.
  25. Schofield, P., Mbugua, D. M., Pell, A. N., 2001, Analysis of condensed tannins: A Review, Anim. Feed Sci. Technol., 91, 21-40. https://doi.org/10.1016/S0377-8401(01)00228-0
  26. Serafini, M., Del Rio, D., 2013, Understanding the association between dietary antioxidants, redox status and disease: Is the total antioxidant capacity the right tool?, Redox Rep., 9, 145-152.
  27. Steinmetz, K. A., Potter, J. K., 1991, Vegetables, fruit, and cancer. I. Epidemiology, Cancer Causes Control, 2, 325-357. https://doi.org/10.1007/BF00051672
  28. Villano, D., Fernandez-Pachon, M. S., Moya, M. L., Troncoso, A. M., Garcia-Parrilla, M. C., 2007, Radical scavenging ability of polyphenolic compounds towards DPPH free radical, Talanta, 71, 230-235. https://doi.org/10.1016/j.talanta.2006.03.050
  29. Walker, R. B., Everette, J. D., 2009, Comparative reaction rates of various antioxidants with ABTS radical cation, J. Agric. Food Chem., 57, 1156-1161. https://doi.org/10.1021/jf8026765
  30. Wolfenden, B. S., Willson, R. L., 1982, Radical-cations as reference chromogens in kinetic studies of ono-electron transfer reactions: Pulse radiolysis studies of 2, 2 -azinobis-(3-ethylbenzthiazoline-6-sulphonate), J. Chem. Soc., 2, 805-812.
  31. Yamauchi, M., Tsuruma, K., Imai, S., Nakanishi, T., Umigai, N., Shimazawa, M., Hara, H., 2011, Crocetin prevents retinal degeneration induced by oxidative and endoplasmic reticulum stresses via inhibition of caspase activity, European J. Pharmacol., 650, 110-119. https://doi.org/10.1016/j.ejphar.2010.09.081