Journal of Microbiology and Biotechnology

  • 제27권8호
  • /
  • Pages.1500-1512
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  • 2017
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Optimization of Siderophore Production by Bacillus sp. PZ-1 and Its Potential Enhancement of Phytoextration of Pb from Soil

  • Yu, Sumei (College of Resources and Environmental Science, Northeast Agricultural University) ;
  • Teng, Chunying (College of Resources and Environmental Science, Northeast Agricultural University) ;
  • Bai, Xin (College of Resources and Environmental Science, Northeast Agricultural University) ;
  • Liang, Jinsong (College of Resources and Environmental Science, Northeast Agricultural University) ;
  • Song, Tao (College of Resources and Environmental Science, Northeast Agricultural University) ;
  • Dong, Liying (College of Resources and Environmental Science, Northeast Agricultural University) ;
  • Jin, Yu (College of Resources and Environmental Science, Northeast Agricultural University) ;
  • Qu, Juanjuan (College of Resources and Environmental Science, Northeast Agricultural University)
  • 투고 : 2017.05.08
  • 심사 : 2017.06.16
  • 발행 : 2017.08.28
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초록

In this study, the siderophore-producing characteristics and conditions of Bacillus sp. PZ-1 were investigated and the enhancement of siderophores on Pb uptake and translocation in Brassica juncea were determined. Results of single factor experiment showed that glucose, pH, and $Pb(NO_3)_2$ could stimulate PZ-1 growth and siderophore production. The maximum siderophore production of 90.52% siderophore units was obtained by response surface methodology optimization at the glucose concentration of 21.84 g/l, pH 6.18, and $Pb(NO_3)_2$ concentration of $245.04{\mu}mol/l$. The type of siderophore was hydroxamate and its concentration in the fermentation broth amounted to $32.24{\mu}g/ml$. Results of pot experiments indicated that the siderophores enhanced B. juncea to assimilate more Pb from soil with the uptake ratio from 1.04 to 2.74, and to translocate more Pb from underground to overground with the TF values from 1.21 to 1.48. The results revealed that Bacillus sp. PZ-1 could produce abundant siderophores and might be potentially used to augment the phytoextraction of Pb from soil.

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