Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Enhanced Internalization of Macromolecular Drugs into Mycobacterium smegmatis with the Assistance of Silver Nanoparticles

  • Sun, Fangfang (Research Institute of Green Science and Technology, Shizuoka University) ;
  • Oh, Sangjin (Department of Cogno-mechatronics Engineering, Pusan National University) ;
  • Kim, Jeonghyo (Department of Cogno-mechatronics Engineering, Pusan National University) ;
  • Kato, Tatsuya (Research Institute of Green Science and Technology, Shizuoka University) ;
  • Kim, Hwa-Jung (Department of Microbiology and Research Institute for Medical Science, College of Medicine, Chungnam National University) ;
  • Lee, Jaebeom (Department of Cogno-mechatronics Engineering, Pusan National University) ;
  • Park, Enoch Y. (Research Institute of Green Science and Technology, Shizuoka University)
  • Received : 2017.01.04
  • Accepted : 2017.06.08
  • Published : 2017.08.28
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Abstract

In this study, silver nanoparticles (AgNPs) were synthesized by the citrate reduction process and, with the assistance of n-hydroxysuccinimide and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, were successfully loaded with the macromolecular drug vancomycin (VAM) to form AgNP-VAM bioconjugates. The synthesized AgNPs, VAM, and AgNP-VAM conjugate were characterized by UV-visible spectroscopy, zeta potential analysis, confocal microscopy, and transmission electron microscopy. The effect of loading VAM onto AgNPs was investigated by testing the internalization of the bioconjugate into Mycobacterium smegmatis. After treatment with the AgNP-VAM conjugate, the bacterial cells showed a significant decrease in UV absorption, indicating that loading of the VAM on AgNPs had vastly improved the drug's internalization compared with that of AgNPs. All the experimental assessments showed that, compared with free AgNPs and VAM, enhanced internalization had been successfully achieved with the AgNP-VAM conjugate, thus leading to significantly better delivery of the macromolecular drug into the M. smegmatis cell. The current research provides a new potential drug delivery system for the treatment of mycobacterial infections.

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