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녹용대보탕 열수 추출물의 실험적으로 유발된 랫트 아급성 출혈성 빈혈에 대한 효과

Ameliorating Effects of Nokyongdaebo-tang on Experimental Subacute Hemorrhagic Anemia in Rats

  • 김정아 (부산대학교 한방병원 여성의학과) ;
  • 김동철 (대구한의대학교 한의과대학 부인과교실)
  • Kim, Jung-Ah (Dep. of Korean Ob & Gy, Pusan National University Korean Medicine Hospital) ;
  • Kim, Dong-Chul (Dept. of Korean Obstetrics & Gynecology, College of Korean Medicine, Daegu Haany University)
  • 투고 : 2017.07.25
  • 심사 : 2017.08.16
  • 발행 : 2017.08.25

초록

목 적: 본 연구는 녹용대보탕이 안와정맥총 방혈로 유발시킨 랫트의 아급성 출혈성 빈혈 모델에 대해 항빈혈 및 조혈촉진 효과를 확인하였다. 방 법: 본 실험에서는 안와 정맥총에서 혈액을 채혈하여 아급성 출혈성 빈혈을 유발시켰으며, 녹용대보탕 추출물을 경구투여하고, 체중 및 조혈 관련 장기(대퇴골 골수, 비장 및 간) 중량, 적혈구 관련 혈액학적 지표의 변화, 혈액 도말 표본의 세포학적 변화, 조혈관련 장기의 조직병리학적 변화와 함께 조혈장기 내 혈구형성 줄기세포의 수적 변화를 면역조직화학적으로 관찰하였다. 또한 철분 보충제의 대표적 부작용인 소화 장애, 특히 변비 유발 여부를 확인하기 위해 탄분을 이용해 위장관 운동성을 평가하였으며, 결장내 분변 표면의 점막 두께와 결장점막 내 점액생산세포 및 결장 점막 두께 변화를 조직병리학적으로 평가하였다. 결 과: 본 실험에서 유발된 아급성 출혈성 빈혈 소견이 모든 세 용량의 녹용대보탕 추출물의 용량 의존적으로 현저히 억제되었다. 또한 세 용량의 녹용대보탕 투여군에서는 정상 대조군 및 아급성 출혈성 빈혈 대조군에 비해 위장관 운동성 변화 및 결장 및 결장내 분변의 점액성분의 유의미한 변화는 없었다. 결 론: 이상의 결과에서, 녹용대보탕의 경구투여는 아급성 출혈성 빈혈 유발 랫트에서 단순 철분 보충제의 부작용인 소화관 장애 없이, 조혈관련 장기들에서 혈구형성줄기세포의 증식 촉진을 통해 투여 용량 의존적으로 철 결핍성 빈혈의 증상을 현저히 완화시키는 것으로 관찰되었다. 따라서 녹용대보탕은 새로운 개념의 조혈촉진 빈혈치료제로서의 개발 가치가 충분할 것으로 판단된다.

Objectives: The object of this study is to observe the possible ameliorating effects of Nokyongdaebo-tang (NYDBT) on the experimental subacute hemorrhagic anemia (SHA) in rats. Methods: In the present study, SHA in rats was induced by exsanguinations from orbital plexus, and ameliorating effects of NYDBT was observed based on the changes of body and hematopoietic organ (spleen, liver and femur) weights, red blood cell (RBC) related hematological values, smear cytology, histopathological changes and immunohistochemistrical analysis of hematopoietic stem cells in the femur bone marrow, liver and spleen. In addition, the gastrointestinal motility and the surface mucosa thicknesses of remnant fecal pellets in the colon lumen, mucosa thicknesses and the mucous producing cell numbers in the colonic mucosa were analyzed to observe the digestive disorders, especially on the constipation, the major discomfort problems in iron supplement. Results: SHA related abnormal anemic signs were markedly and dose-dependently inhibited by oral administration of NYDBT 500, 250 and 125 mg/kg in a condition of this experiment. In addition, no meaningful changes on the gastrointestinal motilities and mucous component on the colon and remnant feces were noticed in all three different dosages of NYDBT treated rats as compared with intact vehicle and SHA control rats in this study. Conclusions: It, therefore, is expected that NYDBT will be promising as a novel alternative hematopoietic and therapeutic agent for anemia.

키워드

참고문헌

  1. Shin TF. Steps to Internal Medicine 1. Seoul:Jungdam. 2005:29-66.
  2. HaBelmann J, Dornbusch J. A rare cause of a chronic bleeding in the upper gastrointestinal tract. Chirurg. 2013;84(4):322-4. https://doi.org/10.1007/s00104-013-2469-0
  3. Bernardi LA, et al. The association between subjective assessment of menstrual bleeding and measures of iron deficiency anemia in premenopausal African-American women: a cross-sectional study. BMC Womens Health. 2016;16(1):50. https://doi.org/10.1186/s12905-016-0329-z
  4. Geisser P. Safety and efficacy of iron(III)-hydroxide polymaltose complex/a review of over 25 years experience. Arzneimittelforschung. 2007;57(6A):439-52.
  5. Feltrin C, et al. Effect of soluble fiber pectin on growth and intestinal iron absorption in rats during recovery from iron deficiency anemia. Biol Trace Elem Res. 2009;129(1-3):221-8. https://doi.org/10.1007/s12011-008-8307-4
  6. Shin CH. Catechetical Basics of Korean medicine. Seoul:Seongbosa. 1988:240.
  7. Kim WH. Byeonjeungronchi of internal organs(臟腑辨證論治). Seoul:Seongbosa. 1985:60-2.
  8. Lee C. Uihagimmun(醫學入門). Seoul:Bubin. 2009:1900.
  9. Cheong MH, Jung IC, Lee SR. The neuroprotective effects of Nokyongdaebo-tang (Lurongdabutang) treatment in pathological Alzheimer's disease model of neural tissues. J Oriental Neuropsychiatry. 2009;20(2):1-17.
  10. Lee SW, et al. Preventive effects of Nokyongdaebotang on hyperlipidemia rats. Kor J Oriental Preventive Medical Society. 2003;7(2):107-19.
  11. Lee KA, Chung HY. Biological activities of a Korean traditional prescription, Nogyongdaebotang. J Korean Soc Food Sci Nutr. 2004;33(1):28-33. https://doi.org/10.3746/jkfn.2004.33.1.028
  12. Bae NY, Ahn TW. The anti-oxidative effect of oral administration of NYD (Nocyongdaebo-tang) in oxidized rats induced by AAPH. J Sasang Constitutional Medicine. 2007;19(2):155-69.
  13. Lee SY, Ahn TW. Anti-aging effect of Tae-Eumin's Nocyongdaebo-tang (NYD) in aged rats. J Sasang Constitutional Medicine. 2008;20(2):58-71.
  14. Pulina MO, et al. Effect of lactoferrin on consequences of acute experimental hemorrhagic anemia in rats. Bull Exp Biol Med. 2010;149(2):219-22. https://doi.org/10.1007/s10517-010-0911-6
  15. Sagar L, Sehgal R, Ojha S. Evaluation of antimotility effect of Lantana camara L. var. acuelata constituents on neostigmine induced gastrointestinal transit in mice. BMC Complement Altern Med. 2005;5:18. https://doi.org/10.1186/1472-6882-5-18
  16. Choi JS, et al. Synergistic effect of fermented rice extracts on the probiotic and laxative properties of yoghurt in rats with loperamide-induced constipation. Evid Based Complement Alternat Med. 2014;2014:878503.
  17. Song MY. Effect of low molecular weight fucoidan on genotoxicity and chronic cisplatin-induced delayed gastrointestinal motility. Seoul:Konkuk University. 2013:36-8.
  18. Price EA, et al. Anemia in older persons: etiology and evaluation. Blood Cells Mol Dis. 2011;46(2):159-65. https://doi.org/10.1016/j.bcmd.2010.11.004
  19. Wu D, et al. Traditional Chinese formula, lubricating gut pill, stimulates cAMP-dependent Cl(-) secretion across rat distal colonic mucosa. J Ethnopharmacol. 2010;134(2):406-13. https://doi.org/10.1016/j.jep.2010.12.031
  20. Tang X, et al. Antigen-retrieval procedure for bromodeoxyuridine immunolabeling with concurrent labeling of nuclear DNA and antigens damaged by HCl pretreatment. J Neurosci. 2007;27(22):5837-44. https://doi.org/10.1523/JNEUROSCI.5048-06.2007
  21. Levene A. Pathological factors influencing excision of tumours in the head and neck. Part I. Clin Otolaryngol Allied Sci. 1981;6(2):145-51. https://doi.org/10.1111/j.1365-2273.1981.tb01800.x
  22. Kang SJ, et al. Fermentation with Aquilariae Lignum enhances the anti-diabetic activity of green tea in type II diabetic db/db mouse. Nutrients. 2014;6(9):3536-71. https://doi.org/10.3390/nu6093536
  23. Moghadam AM, et al. Relationship between blood donors' iron status and their age, body mass index and donation frequency. Sao Paulo Med J. 2013;131(6):377-83. https://doi.org/10.1590/1516-3180.2013.1316554
  24. Scholbach J, et al. Comparison of hematopoietic stem cells derived from fresh and cryopreserved whole cord blood in the generation of humanized mice. PLoS One. 2012;7(10):e46772. https://doi.org/10.1371/journal.pone.0046772
  25. Melikian AL, et al. Hereditary spherocytic hemolytic anemia in an adult with the formation of ectopic foci of extramedullary hemopoiesis in the chest. Ter Arkh. 2010;82(7):72-5.
  26. Baranski GM, et al. ${\beta}$-blockade protection of bone marrow following trauma: the role of G-CSF. J Surg Res. 2011;170(2):325-31. https://doi.org/10.1016/j.jss.2011.03.059
  27. Wintola OA, Sunmonu TO, Afolayan AJ. The effect of Aloe ferox Mill. in the treatment of loperamide-induced constipation in Wistar rats. BMC Gastroenterol. 2010;10:95. https://doi.org/10.1186/1471-230X-10-95