DOI QR코드

DOI QR Code

Effects of Adenophorae Radix(AR) on Atopic Dermatitis(AD) Induced by DNCB in Mice

사삼이 DNCB로 유발된 생쥐의 아토피 피부염에 미치는 영향

  • Lim, Kyeong-Min (Department of Oriental Medical Ophthalmology, Otolaryngology and Dermatology, College of Korean Medicine, Dong-shin University) ;
  • Ko, Hong-Je (Department of Acupuncture and Moxibustion, College of Korean Medicine, Dong-shin University) ;
  • Choi, Jung-Hwa (Department of Oriental Medical Ophthalmology, Otolaryngology and Dermatology, College of Korean Medicine, Dong-shin University) ;
  • Park, Soo-Yeon (Department of Oriental Medical Ophthalmology, Otolaryngology and Dermatology, College of Korean Medicine, Dong-shin University) ;
  • Kim, Jong-Han (Department of Oriental Medical Ophthalmology, Otolaryngology and Dermatology, College of Korean Medicine, Dong-shin University) ;
  • Jung, Min?Yeong (Department of Oriental Medical Ophthalmology, Otolaryngology and Dermatology, College of Korean Medicine, Dong-shin University)
  • 임경민 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 고홍제 (동신대학교 한의과대학 침구과학교실) ;
  • 최정화 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 박수연 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 김종한 (동신대학교 한의과대학 안이비인후피부과학교실) ;
  • 정민영 (동신대학교 한의과대학 안이비인후피부과학교실)
  • Received : 2017.07.11
  • Accepted : 2017.07.30
  • Published : 2017.08.25

Abstract

Objectives : Adenophorae Radix(AR) is the dried root of Adenphora triphylla var. japonica HARA. AR has the efficacy of clarifying lung, emitting pus, expelling wind, stopping pruitus and so on. Recently, various studies are being done about anti-oxidative, immunomodulatory and anti-inflammatory effects of AR. So, we expected AR has an availability that can improve symptoms of atopic dermatitis(AD). Methods : In this study, we measured body weight, weight of ear, thickness of ear skin and dorsum skin, symptom score, reproduction rate of splenocytes in vivo and in vitro of mouse with AD induced by DNCB. We experimented with five groups of 9 mice, such as normal group, control group, AR spread(ARS) group, AR feeding(ARF) group, AR spread&feeding(ARSF) group. Results : Control group of body weight significantly reduced and APSF group significantly increased in the first and second week. In weight of ear and thickness of ear skin and dorsum skin, control group significantly increased and ARSF group significantly decreased. In macrophotography viewpoint of dorsal skin, ARS group, ARF group and ARSF group decreased severe pigmented skin lesion, erythema and desquamation as compared with control group. Especially ARSF group showed significant drop. ARS group, ARF group and ARSF group compared to control group were improved in histopathology observation. In vitro and in vivo, treatment group of AR increased proliferation rate of splenocytes, specially in vitro significantly. Conclusions : This experiment indicates Adenophorae Radix extracts is effective on AD induced by DNCB in mice.

Keywords

References

  1. The Korean Academy of Asthma, Allergy and Clinical Immunology. Asthma and Allergic Diseases. Seoul:Yeomoongak. 2002:25,99, 373-91.
  2. Korean Dermatological Association. Dermatology. Vol 6. Seoul:MED BOOK CO., LTD. 2013:202.
  3. Jung JY, Ha CM. Primary Care of Skin Disease. Vol 2. Seoul:MD world publisher. 2004:90-109.
  4. Dan Longo, Authony Fauci, Dennis Kasper, Stephen Hauser, J Jameson, Joseph Loscalzo. Harrison's Principles of Internal Medicine. Vol 2. 18th ed. Seoul:MIP. 2013:383-4.
  5. Kim KS, Kim MD, Kim YB, Kim JH, Kim JH, Lee WC, et al. Silyong Dongseo-uihag Imsangchongseo(Vol. 6). Seoul:Jung-Dam Pub, Inc. 2001:47-52.
  6. Yuanfang C. Je Bung Won Hu Ron. Sanghai:Chi Wen Bookstore. 1965:505.
  7. Wu Q. Yi Zong Jin Jian. Vol 3. Beijing:Ren Min Wei Sheng Publishing Co., 1982:443-4, 451-2.
  8. Gong NM, Ji SY. A Literature study about comparison of Eastern-Weatern medicine on the Atopic dermatitis. Journel Korean Med Ophthalmol, Otolaryngol, Dermatol. 1999;12(1):241-53.
  9. Textbook compilation committee of National university korean medicine Dermatology & Surgery. Oriental Medical Dermatology & Surgery. Busan:Sunwoo publishing. 2007:355-61.
  10. Seo MS, Kim GJ. The Study on Atopic Dermatitis Papers Published in the Journal of Korean Oriental Ophthalmology & Otolaryngology & Dermatology. Journal Korean Med Ophthalmol, Otolaryngol, Dermatol. 2009;22(3):108-21.
  11. MK Shin. Clinical Traditional Herbalogy. Seoul:Yeong-Lim Pub. 2006:270-2.
  12. Shin Wen Feng Pub Inc. A new edition of Chinese medicine unabridged dictionary. Taipei:Shin Wen Feng Pub Inc. 1971:1203-5.
  13. Heo Jun. Donguibogam. Seoul:Namsungdang. 1994:716-7.
  14. JM Chen. Ben Cao Meng Quan. Beijing: People's medical Publishing House Co. LTD. 1988:119.
  15. BX Wang. Modern Chinese Pharmacology. Tianjin:Tianjin Science and Technology Press. 1997:1363-4.
  16. Kim JH, Ahn DG. A comparative study on rate of Free Sugar and effectiveness of Radix and Radix Adenophorae. The Korea Journal of Herbology. 1992;7(1)3-13.
  17. Kim SH. Development of hematopoietic factor from natural products. University of Ulsan. 1993.
  18. Shin DH. The Experimental Studies on the Immunomodulational Effects of Adenophorae Radix. University of Daejeon. 1999.
  19. Ham YA. Studies on screening of biological activities from Adenophorae Radix (Adenophora triphylla Var. japonica Hara). University of Kangwon. 2007.
  20. Min SH, Han HS, Lee YJ. Study on the Anti-oxidative Effects of Adenophorae Radix, Codonopsis lanceolatae Radix and Glehniae Radix Cum Rhizoma on Liver Cells Isolated from Oxidatively Stressed Rat. The Korea Journal of Herbology. 2009;24(3)109-19.
  21. Lee YM. A study on the influence of Adenophora Radix extract on hair growing. University of Sungshin. 2008.
  22. Lim NY. Inhibitory Effect of Methanolic Extract from Adenophorae Radix on Melanogenesis. University of Wonkwang. 2004.
  23. Kang GU. Study on the antiinflammatory effect and the isolation of new constituents from the roots of Adenophora triphylla. University of Dong-guk. 2011.
  24. Herbological professor of National university korean medicine. Herbalogy. Seoul: Youngimsa. 1999:587-8.
  25. Wysocki LJ, Sato VL. "Planning" for lymphocytes; a method for cell selection. Proc Natl Acad Sci. 1978;75(6):2844-8. https://doi.org/10.1073/pnas.75.6.2844
  26. Mizel SB, Rosenstreich DL. Regulation of lymphocyte-activating factor (LAF) production and secretion in P388D1 cells; identification of high molecular weight precursors of LAF. J. Immunol. 1979;122(6):2173-9.
  27. Color illustrated guide of Dermatological compilation committee. Color illustrated guide of Dermatology. Seoul:Jung-dam Publishing. 1999:54-63.
  28. Textbook compilation committee of Korean Dermatological Association. Dermatology. Seoul:Ryo-Moon-Gak publishing. 2000:161.
  29. Ki YU. Diagnosis & Treatment of Skin disease. Gwangju:Seo-Gwang Medicine. 1988:23.
  30. Hong CS. Steps to Internal Medicine - Immunity, Allergic disease Part 7. Seoul: Jung-dam Publishing. 2008:41-7,55-8.
  31. Kang Wonhyeong. Atlas Of Skin Diseaes. Seoul:Hanmibook. 2006:9,71-92.
  32. Compilation Committee Of Chinese Medicine Dictionary. Chinese Medicine Dictionary. Vol 1. Seoul:Jung-dam Publishing. 1997:682-6.
  33. Korean Medicinal Botany Society. General Medicinal Botany. Seoul:Hakchangsa. 2002:290.
  34. Jo WU. Confucian classics of medical herbs. Shanghai:Shanghai Science Technology Publisher. 1987:123.
  35. De BI, Schols A, Koerts-deLang E, Wouters E, DEutz N. De novo glutamine synthesis induced by corticosteroids in vivo in rats is secondary to weight loss. Clin Nutr. 2004;23(5):1035-42. https://doi.org/10.1016/j.clnu.2004.01.004
  36. Kim JK, Kim YH, Lee HH, Lim SS, Park KW. Effect of Scrophularia Buergeriana Extract on the Degranulation of Mast Cells and Ear Swelling Induced by Dinitrofluorobenzene in Mice. Inflammation. 2012.;35(1):183-91. https://doi.org/10.1007/s10753-011-9304-x
  37. Mezard P, Rosieres A, Krasteva M, Berard F, Dubois B, Kaiserlian D, et al. F. Allergic contact dermatitis. Eur J Dermatol. 2004;14(5):284-95.
  38. Rhu HS, Kim J, Park SC, Kim HS. Enhancing Effect of Zingiber Officinale Roscoe Extracts on Mouse Spleen and Macrophage Cells Activation. Korean Journal of food and nutrition. 2004;37(9):780-5.