Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지
DOI QR코드

DOI QR Code

Enzyme hydrolysate of silk protein suppresses tert-butyl hydroperoxide-induced hepatotoxicity by enhancing antioxidant activity in rats

  • Suh, Hyung Joo (Department of Food and Nutrition, Korea University) ;
  • Kang, Bobin (Department of Food and Nutrition, Korea University) ;
  • Kim, Chae-Young (Department of Food and Nutrition, Korea University) ;
  • Choi, Hyeon-Son (Department of Food Science and Technology, Seoul Women's University)
  • Received : 2017.05.31
  • Accepted : 2017.07.20
  • Published : 2017.07.30
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of current study is to investigate the beneficial effect of enzyme (Alcalase) hydrolysates of silk protein in rat. Alcalase-treated silk protein hydrolysate (ATSH) itself did not show any cytotoxicity on the hepatic tissues and blood biochemistry, similar to the normal condition. ATSH played a protective role in tert-butyl hydroperoxide (t-BHP)-induced hepatotoxicity and liver damage. The values of AST (aspartate aminotransferase) and ALT (alanine aminotransferase), which are the indicators of the liver function, were effectively alleviated with the ATSH treatment in a dose dependent manner. The level of Lactate dehydrogenase (LDH) and Malondialdehyde (MDA), which were increased with t-BHP treatment, were significantly reduced by ATSH. High dose of ATSH (2 g/kg) reduced the t-BHP-induced LDH release by 48%. Antioxidant and antioxidant enzymes in liver cells were significantly increased by ATSH treatment in their level and activities. ATSH (2 g/kg) increased glutathione (GSH), an intracelluar antioxidant, by 2.5-fold compared with the t-BHP treated group. The activities of glutathione-s-transferase (GST), superoxide dismutase (SOD), and catalase were also elevated by 38%, 60%, and 45%, respectively, with ATSH (2 g/kg) treatment. The antioxidative effect of ATSH was recapitulated to the protection from t-BHP induced liver damages in hematoxylin and eosin (H&E) staining. Thus, ATSH might be used as a hepatoprotective agent.

Keywords

References

  1. Kim J, Hwang H, Park J, Yun HY, Suh H, Lim K (2014) Silk peptide treatment can improve the exercise performance of mice. J Int Soc Sports Nutr, 11, 35 https://doi.org/10.1186/1550-2783-11-35
  2. Lee SH, Cho BN, Han CK, Joo SS (2002) Physiologic functionality of silk peptides- focus on antioxidant and immune function. Food Science and Industry, 35, 57-61
  3. Hwang E, Kang B, Kim B, Lee HJ (2001) Protein quality evaluation and effect of plasma lipid contents of acid hydrolysates of cocoon in rats fed by high cholesterol, high triglyceride and high sucrose diet. J Korean Soc Food Sci Nutr, 30, 1004-1009
  4. Zhaorigetu S, Yanaka N, Sasaki M, Watanabe H, Kato N (2003) Inhibitory effects of silk protein, sericin on UVB-induced acute damage and tumor promotion by reducing oxidative stress in the skin of hairless mouse. J Photochem Photobiol B, 71, 11-17 https://doi.org/10.1016/S1011-1344(03)00092-7
  5. Kim AJ, Kim SY, Choi MK, Kim MH, Han MR, Chung KS (2005) Effects of mulberry leaves powder on lipid metabolism in high cholesterol-fed rats. Korean J Food Sci Technol, 37, 636-641
  6. Kim DW, Hwang HS, Kim DS, Sheen SH, Heo DH, Hwang G, Kang SH, Kweon H, Jo YY, Kang SW, Lee KG, Park KW, Han KH, Park J, Eum WS, Cho YJ, Choi HC, Choi SY (2011) Effect of silk fibroin peptide derived from silkworm Bombyx mori on the anti-inflammatory effect of Tat-SOD in a mice edema model. BMB Rep, 44, 787-792 https://doi.org/10.5483/BMBRep.2011.44.12.787
  7. Abdel-Misih SRZ, Bloomston M (2010) Liver anatomy. Surg Clin N Am, 90, 643-653 https://doi.org/10.1016/j.suc.2010.04.017
  8. Davis GL, Albright JE, Cook SF, Rosenberg DM (2003) Projecting future complications of chronic hepatitis C in the United States. Liver Transplant, 9, 331-338 https://doi.org/10.1053/jlts.2003.50073
  9. Cesaratto L, Vascotto C, Calligaris S, Tell G (2004) The importance of redox state in liver damage. Ann Hepatol, 3, 86-92
  10. Baud L, Ardaillou R (1993) Involvement of reactive oxygen species in kidney damage. Br Med Bull, 49, 621-629 https://doi.org/10.1093/oxfordjournals.bmb.a072635
  11. Griffiths HR, Dowling EJ, Sahinoglu T, Blake DR, Parnham M, Lunec J (1992) The selective protection afforded by ebselen against lipid peroxidation in an ROS-dependent model of inflammation. Agents Actions, 36, 107-111 https://doi.org/10.1007/BF01991237
  12. Halliwell B, Gutteridge JMC, Cross CE (1992) Free radicals, antioxidants, and human disease: where are we now?. J Lab Clin Med, 119, 598-620
  13. McCord JM (1988) Superoxide dismutase: the first twenty years (1968-1988). Free Radic Biol Med, 5, 363-369 https://doi.org/10.1016/0891-5849(88)90109-8
  14. Meister A (1988) Glutathione metabolism and its selective modification. J Biol Chem, 263, 17205-17208
  15. Spector A (2009) Review: Oxidative stress and disease. J Ocul Pharmacol Ther, 16, 193-201
  16. Kim EY, Hong KB, Suh HJ, Choi HS (2015) Protective effects of germinated and fermented soybean extract against tert-butyl hydroperoxide-induced hepatotoxicity in HepG2 cells and in rats. Food Funct, 6, 3512-3521 https://doi.org/10.1039/C5FO00785B
  17. Lock JF, Reinhold T, Malinowski M, Pratschke J, Neuhaus P, Stockmann M (2009) The costs of postoperative liver failure and the economic impact of liver function capacity after extended liver resection-a single-center experience. Langenbecks Arch Surg, 394, 1047-1056 https://doi.org/10.1007/s00423-009-0518-4
  18. Kim JH, Suh HJ, Choi HS (2017) Protective effect of silk protein hydrolysates against tert-BHP induced liver damage. Korean J Food Preserv, 24, 107-115 https://doi.org/10.11002/kjfp.2017.24.1.107
  19. Wang CJ, Wang JM, Lin WL, Chu CY, Chou FP, Tseng TH (2000) Protective effect of Hibiscus anthocyanins against tert-butyl hydroperoxide-induced hepatic toxicity in rats. Food Chem Toxicol, 38, 411-416 https://doi.org/10.1016/S0278-6915(00)00011-9
  20. Reed DJ (1986) Regulation of reductive processes by glutathione. Biochem Pharmacol, 35, 7-13 https://doi.org/10.1016/0006-2952(86)90545-9
  21. Habig WH, Pabst MJ, Jakoby WB (1974) Glutathione S-transferases: The first enzymatic step in mercapturic acid formation. J Biol Chem, 249, 7130-7139
  22. Aebi H, Suter H, Feinstein RN (1968) Activity and stability of catalase in blood and tissues of normal and acatalasemic mice. Biochem Genet, 2, 245-251 https://doi.org/10.1007/BF01474764
  23. Durackova Z, Labuda J (1995) Superoxide dismutase mimetic activity of macrocyclic Cu(II)-tetraanhydroaminobenzaldehyde (TAAB) complex. J Inorg Biochem, 58, 297-303 https://doi.org/10.1016/0162-0134(94)00065-I
  24. Lee HS, Won NH, Kim KH, Lee H, Jun W, Lee KW (2005) Antioxidant effects of aqueous extract of Terminalia chebula in vivo and in vitro. Biol Pharm Bull, 28, 1639-1644 https://doi.org/10.1248/bpb.28.1639
  25. Cassidy WM, Reynolds TB (1994) Serum lactic dehydrogenase in the differential diagnosis of acute hepatocellular injury. J Clin Gastroenterol, 19, 118-121 https://doi.org/10.1097/00004836-199409000-00008
  26. Chiang WD, Huang CY, Paul CR, Lee ZY, Lin WT (2016) Lipolysis stimulating peptides of potato protein hydrolysate effectively suppresses high-fat-diet-induced hepatocyte apoptosis and fibrosis in aging rats. Food Nutr Res, 60, 31417 https://doi.org/10.3402/fnr.v60.31417
  27. Chung YI, Bae IY, Lee JY, Chun HS, Lee HG (2009) Protective effects of branched-chain amino acid (BCAA)-enriched corn gluten hydrolysates on ethanolinduced hepatic injury in rats. Korean J Food Sci Technol, 41, 706-711
  28. Kamoun Z, Kamoun AS, Bougatef A, Kharrat RM, Youssfi H, Boudawara T, Chakroun M, Nasri M, Zeghal N (2017) Hepatoprotective and nephroprotective effects of sardinelle (Sardinella aurita) protein hydrolysate against ethanol-induced oxidative stress in rats. Environ Sci Pollut Res Int, 24, 1432-1441 https://doi.org/10.1007/s11356-016-7424-4
  29. Draper HH, Hadley M (1990) Malondialdehyde determination as index of lipid Peroxidation. Method Enzymol, 186, 421-431
  30. Han BK, Park Y, Choi HS, Suh HJ (2016) Hepatoprotective effects of soluble rice protein in primary hepatocytes and in mice. J Sci Food Agric, 96, 685-694 https://doi.org/10.1002/jsfa.7153
  31. Cai X, Yan A, Fu N, Wang S (2017) In vitro antioxidant activities of enzymatic hydrolysate from Schizochytrium sp. and its hepatoprotective effects on acute alcoholinduced liver injury in vivo. Mar Drugs, 15, 115 https://doi.org/10.3390/md15040115
  32. Lushchak VI (2012) Glutathione homeostasis and functions: potential targets for medical interventions. J Amino Acids, 2012, 736837
  33. Nasri R, Abdelhedi O, Jemil I, Daoued I, Hamden K, Kallel C, Elfeki A, Lamri-Senhadji M, Boualga A, Nasri M, Karra-Chaabouni M (2015) Ameliorating effects of goby fish protein hydrolysates on high-fat-high-fructose diet-induced hyperglycemia, oxidative stress and deterioration of kidney function in rats. Chem Biol Interact, 242, 71-80 https://doi.org/10.1016/j.cbi.2015.08.003
  34. Han CH, Lin YF, Lin YS, Lee TL, Huang WJ, Lin SY, Hou WC (2014) Effects of yam tuber protein, dioscorin, on attenuating oxidative status and learning dysfunction in D-galactose-induced BALB/c mice. Food Chem Toxicol, 65, 356-363 https://doi.org/10.1016/j.fct.2014.01.012
  35. Pacheco MTB, Sgarbieri VC (2005) Effect of different hydrolysates of whey protein on hepatic glutathione content in mice. J Med Food, 8, 337-342 https://doi.org/10.1089/jmf.2005.8.337
  36. Guicciardi ME, Malhi H, Mott JL, Gores GJ (2013) Apoptosis and necrosis in the liver. Compr Physiol, 3, 977-1010
  37. Rock KL, Kono H (2008) The inflammatory response to cell death. Annu Rev Pathol: Mech Dis, 3, 99-126 https://doi.org/10.1146/annurev.pathmechdis.3.121806.151456