Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Growth Inhibitory Effect of Extracts of Propolis on Epithelial Ovarian Cancer Cells

상피성 난소암 세포에서 프로폴리스 추출물의 세포 증식 저해 효과

  • Yang, Ga Ram (School of Korean Medicine, Pusan National University) ;
  • Yoon, Kyung Mi (School of Korean Medicine, Pusan National University) ;
  • Oh, Hyun Ho (School of Korean Medicine, Pusan National University) ;
  • Kim, Min Sung (Institute of Marine BioTechnology Pusan National University) ;
  • Hwang, Tae Ho (Department of Pharmacology, School of Medicine, Pusan National University) ;
  • An, Won Gun (School of Korean Medicine, Pusan National University)
  • 양가람 (부산대학교 한의학전문대학원) ;
  • 윤경미 (부산대학교 한의학전문대학원) ;
  • 오현호 (부산대학교 한의학전문대학원) ;
  • 김민성 (부산대학교 해양생물기술연구소) ;
  • 황태호 (부산대학교 의학전문대학원 약리학교실) ;
  • 안원근 (부산대학교 한의학전문대학원)
  • Received : 2017.06.09
  • Accepted : 2017.07.24
  • Published : 2017.07.30
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Abstract

Propolis is a natural product collected from plants by honey bees product used extensively in traditional medicine for its antioxidant, anti-inflammatory, immunomodulatory and anti-cancer effects. Propolis exhibits a broad spectrum of biological activities because it is a complex mixture of natural substances. Ovarian cancer is the second most common newly diagnosed cancer from all cancers among women in Korea and the leading cause of death from gynecological malignancies. While most ovarian cancer patients initially respond to surgical debulking and chemotherapy, patients later succumb to the disease. Thus, there is an urgent need to test novel therapeutic agents to counteract the high mortality rate associated with ovarian cancer. In this study, we investigated the anti-cancer properties and the active mechanism of Australian propolis in human epithelial ovarian cancer A2780 cells. Our data revealed that propolis showed a cytotoxic activity in a dose-dependent manner. Flow cytometric analysis for cell cycle arrest and apoptosis using propidium iodide staning and annexin V-FITC indicated that propolis could induce cycle arrest in the G0/G1 phase and apoptosis in a dose-dependent manner on human epithelial ovarian cancer cells. These results suggest that the Australian propolis is potential alternative agent on ovarian cancer prevention and treatment.

Propolis는 꿀벌들이 나무로부터 수집한 천연물로서 항산화, 항염증, 항암 효과를 가지고 있어 전통의학에서 사용되고 있으며, 이러한 생리활성은 여러 가지 유용성분들이 혼합된 것과 관련이 있다. 난소암은 우리나라 여성에서 두 번째로 발병률이 높은 암이다. 대부분의 난소암 환자들은 초기에 수술적 기법과 항암요법에 우수하게 반응하지만, 항암제 내성에 의한 재발이 발생하게 되면 항암요법제에 의한 반응률이 매우 저조하여 높은 사망률을 보인다. 따라서, 난소암의 높은 치사율을 극복하기 위한 새로운 치료제 및 항암보조제의 개발이 필요하다. 본 연구에서는 인체 상피성 난소암 세포주인 A2780를 이용하여 Austalian propolis의 항암 효과와 활성기전을 조사하였다. Propolis 추출물은 농도 의존적으로 난소암 세포의 증식을 억제했으며. Flow cytometric 분석을 통해 G0/G1기에서 세포 주기 억제와 apoptosis 유도 효과를 확인하였다. 이러한 결과는 Austalian propolis의 인간 난소암에 대한 예방과 치료를 위한 보조제로서의 가능성을 제시한다.

Keywords

References

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