The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anti-inflammatory Effects of Jema-sunghyangjungkisan and Yeoldahanso-tang

제마성향정기산과 열다한소탕의 항염증 효능 비교 연구

  • Shim, Eun Hyoung (KM Fundamental Research Division, Korea Institute of Oriental Medicine) ;
  • You, Sooseong (KM Fundamental Research Division, Korea Institute of Oriental Medicine) ;
  • Lee, Hoyoung (KM Fundamental Research Division, Korea Institute of Oriental Medicine)
  • 심은형 (한국한의학연구원 한의기반연구부) ;
  • 유수성 (한국한의학연구원 한의기반연구부) ;
  • 이호영 (한국한의학연구원 한의기반연구부)
  • Received : 2017.04.25
  • Accepted : 2017.07.15
  • Published : 2017.07.30
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Abstract

Objectives : Jema-sunghyangjungkisan (JSGS, Jima Xingxiang Zhengqi san) and yeoldahanso-tang (YDHST, Reduo hanshao decoction) are traditional herbal formulas which commonly used to prevent and treat stroke in traditional korean medicine. However, JSGS and YDHST extracts have not been previously reported to have anti-inflammatory effects. Therefore, We measured the anti-inflammatory effects of JSGS and YDHST extracts on lipopolysaccharide (LPS)-stimulated murine macrophage cell line, RAW 264.7 cells. Methods : To investigate the anti-inflammatory activities of JSGS and YDHST extracts, tumor necrosis factor-alpha ($TNF-{\alpha}$), interleukin (IL)-6, nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) were examined in RAW 264.7 cells with LPS of $1{\mu}g/m{\ell}$. Results : JSGS and YDHST extracts did not have any cytotoxicity in RAW 264.7 cells. $TNF-{\alpha}$ concentration decreased 49.67% at $500{\mu}g/m{\ell}$ by JSGS but, YDHST has no statistically significant effect at all concentration. IL-6 accumulation on JSGS and YDHST extracts in LPS-stimulated RAW 264.7 cells reduced 22.03% and 41.44% at $500{\mu}g/m{\ell}$ respectively. In addition, JSGS has no inhibitory effects on NO accumulation and YDHST reduced 10.08% at $500{\mu}g/m{\ell}$. Moreover, JSGS and YDHST treatment does-dependently reduced the $PGE_2$ production. In particular, YDHST ($500{\mu}g/m{\ell}$) extract was more effective compared with $10ng/m{\ell}$ of indomethacin which is the $PGE_2$ positive control. Conclusions : Our results suggest that treatment of JSGS and YDHST extracts decreased the LPS-stimulated inflammation. Therefore, in the present study, we demonstrated that JSGS and YDHS may be used as a potential anti-inflammatory therapeutic agent.

Keywords

References

  1. Koo BH. Cardiovascular and neurologic disease. Seoul : Seowondang. 1985 : 305-11
  2. Kim YG, Kweon JN, Kim JH. Effect of Sunghyangchungisan on Contractile Reactivity and $Ca^{2+}$ Metabolism in Isolated Rabbit Carotid Artery. Korean J Orient Int Med. 2000 ; 21(3) : 377-88.
  3. Kim HS, Kim YS. Neuroprotective Effect of Sunghyangjungki-San on Focal Cerebral Ischemia Induced by MCAO in Rats. Korean J Physiology & Pathology 2005 ; 20(3) : 596-02.
  4. Kim YJ, Kim BO, Kim HJ, Kwack JJ, Jeon SY, Hong S. A clinical report of CVA with diabetib foot. Korean J Orient Int Med. 2003 ; 24(3) : 727-34.
  5. Kim OS, Kim YJ, Shin HK. Anti-atherosclerotic Effects of Herbal Formulas for Sasang Constitutional Medicine. J Sasang Constit Med. 2012 ; 24(4) : 51-61. https://doi.org/10.7730/JSCM.2012.24.4.51
  6. Bae HS, Kim YS, Cho KH, Lee KS, Kim JJ, Lee HU, Kim DH. Hepatoprotective Activity of Reduohanxiaotang (Yuldahanso-tang) is Related to the Inhibition of $\beta$-Glucuronidase. Am J Chi Med. 2003 ; 31(1) : 111-7. https://doi.org/10.1142/S0192415X03000722
  7. Kim MS, Park SS. Effects of yuldahansotang on rat skin occurred the allergic contact dermatitis. J of Sasang Const Med. 2002 ; 14(3) : 97-113.
  8. Jung HS, Lee JY. A study on the alteration of general characteristics and therapeutic effect of GamiYeoldahansotang (加味熱多寒少湯) in patients with Atopic Dermatitis. 2001 ; 15(2) : 177-88.
  9. Kim JH, Kang KW, Yu BC, Choi SM, Baek HK, Lim SM, Seol IC, Kim YS. A Case-Control Study on Blood Lipids as a Risk Factor of Stroke. Korean J Orient Int Med. 2007 ; 28(4) : 830-7.
  10. Yoon YY, Song JH. Korean Death Report in 2014. Seoul : Statistics Korea. 2015.
  11. Kim MJ, Kang BG, An JJ, Jo HK, Yoo HR, Kim YS, Seol IC. Case-Control Study on Relationship of Dampness-phlegm to Blood Lipid Level in Stroke Patients. Korean J Oriental Physiology & Pathology. 2009 ; 23(6) : 1470-9.
  12. Park JK, Choi HJ, Kim DH. A study on the Effect of Kamijesubsungitang on Hyperlipidemia. J Daejeon Orien Med. 2006 ; 15(1) : 19-31.
  13. Min IK, Kim CH, Hwang JW, Park JY, Lee SY, Choi WW, Na BJ, Park SW, Jung WS, Moon SK, Park JM, Ko CN, Cho KH, Kim YS, Bae HS. The relation of Dampness-Phlegm and Metabolic Syndrome in Acute Stroke Patients. J Korean Oriental Med. 2009 ; 30(1) : 109-19.
  14. Woo JM. The Relationship between Epicardial Fat Thickness and Dampness - Phlegm Pattern in the Patients with ischemic stroke. Kyung Hee Univ. 2017.
  15. Mariathasan S, Monack DM. Inflammasome adaptors and sensors : intracellular regulators of infection and inflammation. Nat. Rev. Immunol. 2007 ; 7(1) : 31-40. https://doi.org/10.1038/nri1997
  16. Zedler S, Faist E. The impact of endogenous triggers on trauma-associated inflammation. Curr Opin Crit Care. 2006 ; 12(6) : 595-01. https://doi.org/10.1097/MCC.0b013e3280106806
  17. Allen L-AH, Aderem A. Mechanisms of phagocytosis. Curr opin immunol. 1996 ; 8(1) : 36-40. https://doi.org/10.1016/S0952-7915(96)80102-6
  18. Nathan CF. Secretory products of macrophages. J Clin Invest. 1987 ; 79(2) : 319-26. https://doi.org/10.1172/JCI112815
  19. Salvemini D, Korbut R, Anggard E, Vane J. Immediate elease of a nitric oxide-like factor from bovine aortic endothelial cells by Escherichia coli lipopolysaccharide. Proc. Natl. Acad. Sci. USA. 1990 ; 87(7) : 2593-7. https://doi.org/10.1073/pnas.87.7.2593
  20. Nava E, Palmer RM, Moncada S. The role of nitric oxide in endotoxic shock : effects of NG-monomethyl-L-arginine. J Cardiovasc Pharmacol. 1992 ; 20 (Suppl 12) : S132-4. https://doi.org/10.1097/00005344-199204002-00037
  21. Hilliquin P, Borderie D, Hernvann A, Menkes CJ, Ekindjian OG. Nitric oxide as S-nitrosoproteins in rheumatoid arthritis. Arthritis Rheum. 1997 ; 40(8) : 1512-7. https://doi.org/10.1002/art.1780400820
  22. Lopez-Bojorquez LN, Dehesa AJ Reyes-Teran G. Molecular mechanisms involved in the pathogenesis of septic shock. Med Res Arch. 2004 ; 35(6) : 465-79. https://doi.org/10.1016/j.arcmed.2004.07.006
  23. Lee JM. Longevity & life preservation in oriental medicine. Seoul ; Kyung Hee University Press : 1997
  24. Choi IY, Kim YH, Choi DJ, Han CH, Lee WC, Jun CY, Cho KH, Choi SM. Relationship between Warning Signs and Sasang Constitution(SC) in Early Stroke Patients. Korean J Orient Int Med. 2007 ; 28(4) : 773-40.
  25. Wang Y, Li C, Chang H, Lu LH, Qiu Q, Ouyang YL, Yu JD, Guo SZ, Han J, Wang W. Metabolomic profiling reveals distinct patterns of tricarboxylic acid disorders in blood stasis syndrome associated with coronary heart disease. Chin J Integr Med. 2016 ; 22(8) : 597-04. https://doi.org/10.1007/s11655-015-2401-1
  26. Kang JS, Kim DH, Shin HS, Jo CH, Lee JH, Kang BG, An JJ, Jo HK, Yoo HR, Seol IC, Kim YS. The Study on Relationship of Dampness-Phlegm Tongue Diagnosis to hyperlipidemia in Stroke Patients. The journal of the Korea institute of oriental medical diagnostics. 2009 ; 13(2) : 24-33.
  27. Kang BG, Go HY, Kang KY, Park SW, Kim JC, Go MM, Kim BY, Seol IC, Lee I, Jo HK, Choi SM. Study of The Diagnostic Indicators of Dampness-Phlegm Pattern Identification In Stroke Patients. Korean journal of oriental medicine. 2007 ; 13(2) : 53-8.
  28. Wang J, Wang Q, Li L, Li Y, Zhang H, Zheng L, Yang L, Zheng Y, Yang Y, Peng G, Zhang Y, Han Y. Phlegm-dampness constitution : genomics, susceptibility, adjustment and treatment with traditional Chinese medicine. Am J Chin Med. 2013 ; 41(2) : 253-62. https://doi.org/10.1142/S0192415X13500183
  29. Male D. Cell migration and inflammation. Immunol. 1993 ; 5 : 61-9.
  30. Chao CY, Yeh SL, Lin MT, Chen WJ. Effects of parenteral infusion with fish-oil or safflower-oil emulsion on hepatic lipids, plasma amino acids, and inflammatory mediators in septic rats. Nutr. 2000 ; 16(4) : 284-8. https://doi.org/10.1016/S0899-9007(99)00299-3
  31. Iontcheva I, Amar S, Zawawi KH, Kantarci A, Van Dyke TE. Role for moesin in lipopolysaccharidestimulated signal transduction. Infect Immun. 2004 ; 72(4) : 2312-20. https://doi.org/10.1128/IAI.72.4.2312-2320.2004
  32. Van Snick J. Interleukin-6 : an overview. Annu Rev Immunol. 1990 ; 8 : 253-78. https://doi.org/10.1146/annurev.iy.08.040190.001345
  33. Yoon HJ, Moon ME, Park HS, Im SY, Lee JH, Kim YH. Effects of Chitosamoligosaccharide on the C.albicans-induced inflammatory effect in mice and RAW 264.7 macrophage cells. J Chitin Chitosan. 2007 ; 12(1) : 15-20.
  34. Leng SX, McElhaney JE, Walston JD, Xie D, Fedarko NS, Kuchel GA. ELISA and multiplex technologies for cytokine measurement in inflammation and aging research. J Gerontol A Biol Sci Med Sci. 2008 ; 63(8) : 879-84. https://doi.org/10.1093/gerona/63.8.879
  35. Oh CH. Translation. simple immunology. 3nd rev. ed. seoul : Medical korea. 2006 : 161-200
  36. Moncada S, Palmer RM, Higgs EA. Nitric oxide : physiology, pathophysiology, and pharmacology. Pharmacol Rev. 1991 ; 43(2) : 109-42.
  37. McCann SM, Mastronardi C, de Laurentiis A, Rettori V. The nitric oxide theory of aging revisited. Ann N Y Acad Sci. 2005 ; 1057 : 64-84. https://doi.org/10.1196/annals.1356.064
  38. Radi R, Beckman JS, Bush KM, Freeman BA. Peroxynitrite oxidation of sulfhydryls. The cytotoxic potential of superoxide and nitric oxide. J Biol Chem, 1991 ; 266(7) : 4244-50.
  39. Murakami, A, Ohigashi H. Targeting NOX, INOS and COX-2 in inflammatory cells : chemoprevention using food phytochemicals. Int J Cancer. 2007 ; 121(11) : 2357-63. https://doi.org/10.1002/ijc.23161
  40. Konturek PC, Kania J, Burnat G, Hahn EG, Konturek SJ. Prostaglandins as mediators of COX-2 derived carcinogenesis in gastrointestinal tract. J Physiol Pharmacol. 2005 ; 56 (Suppl 5) : 57-73.
  41. Gravaghi C, La Perle KM, Ogrodwski P, Kang JX, Quimby F, Lipkin M, Lamprecht SA. Cox-2 expression, PGE2 and cytokines production are inhibited by endogenously synthesized n-3 PUFAs in inflamed colon of fat-1 mice. J Nutr Biochem. 2011 ; 22(4) : 360-5. https://doi.org/10.1016/j.jnutbio.2010.03.003