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Distribution of Rhizosphere and Endosphere Fungi on the First-Class Endangered Plant Cypripedium japonicum

  • Gang, Geun-Hye (Species Restoration Technology Institute, Korea National Park Service) ;
  • Cho, Gyeongjun (Division of Applied Life Science (BK21 Plus) and Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Kwak, Youn-Sig (Division of Applied Life Science (BK21 Plus) and Institute of Agriculture & Life Science, Gyeongsang National University) ;
  • Park, Eun-Hee (Species Restoration Technology Institute, Korea National Park Service)
  • Received : 2017.03.23
  • Accepted : 2017.05.15
  • Published : 2017.06.30

Abstract

Endangered native plant habitats and populations are rapidly disappearing because of climate and environmental changes. As a representative, the abundance of the first-class endangered wild plant, Cypripedium japonicum, has been rapidly decreasing in Korea. The purpose of this study was to evaluate the distribution of rhizosphere and endophytic fungi on C. japonicum in its native habitat. A total of 440 rhizosphere and 79 endosphere fungi isolates were isolated and identified on the basis of their molecular characteristics. Sixty-five genera and 119 fungi species were identified in this study. The genus Trichoderma showed the highest abundance among both rhizosphere and endosphere fungi. Mortierella, Hypocrea, and Penicillium spp. were also relatively dominant species on C. japonicum. The community structures of rhizosphere and endosphere fungi were similar, but endosphere fungi showed greater diversity.

Keywords

References

  1. Lee B. Rare plants data book of Korea. Pocheon: Korea National Arboretum; 2009.
  2. Bonfante P, Genre A. Mechanisms underlying beneficial plantfungus interactions in mycorrhizal symbiosis. Nat Commun 2010;1:48.
  3. Shimura H, Sadamoto M, Matsuura M, Kawahara T, Naito S, Koda Y. Characterization of mycorrhizal fungi isolated from the threatened Cypripedium macranthos in a northern island of Japan: two phylogenetically distinct fungi associated with the orchid. Mycorrhiza 2009;19:525-34. https://doi.org/10.1007/s00572-009-0251-4
  4. Sawmya K, Vasudevan TG, Murali TS. Fungal endophytes from two orchid species: pointer towards organ specificity. Czech Mycol 2013;65:89-101.
  5. Sim MY, Youm J, Chung J, Lee B, Koo CD, Eom AH. Characteristic of orchid mycorrhizal fungi from roots of Cypripedium japonicum and C. macranthum. Kor J Mycol 2010;38:1-4. https://doi.org/10.4489/KJM.2010.38.1.001
  6. Zettler LW, Hofer CJ. Propagation of the little club-spur orchid (Platanthera clavellata) by symbiotic seed germination and its ecological implications. Environ Exp Bot 1998;39:189-95. https://doi.org/10.1016/S0098-8472(97)00019-1
  7. Warcup JH. Rhizanthella gardneri (Orchidaceae), its Rhizoctonia endophyte and close association with Melaleuca uncinata (Myrtaceae) in Western Australia. New Phytol 1985;99:273-80. https://doi.org/10.1111/j.1469-8137.1985.tb03656.x
  8. Carroll G. Fungal endophytes in stems and leaves: from latent pathogen to mutualistic symbiont. Ecology 1988;69:2-9. https://doi.org/10.2307/1943154
  9. Nam Y, Hwang BY, Jung DH, Shin DJ, Jung HJ, Hong GP, Kim BB, Song DJ, Park EH. A study on the efficient restoration method of Neofinetia falcata (Thunb.) Hu. as endangered wild species I class. J Agric Life Sci 2016;50:39-49. https://doi.org/10.14397/jals.2016.50.5.39
  10. Lee BH, Han HK, Kwon HJ, Eom AH. Diversity of endophytic fungi isolated from roots of Cypripedium japonicum and C. macranthum in Korea. Kor J Mycol 2015;43:20-5. https://doi.org/10.4489/KJM.2015.43.1.20
  11. Graham J, Marshall B, Squire GR. Genetic differentiation over a spatial environmental gradient in wild Rubus ideaus populations. New Phytol 2003;157:667-75. https://doi.org/10.1046/j.1469-8137.2003.00693.x
  12. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetic. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: a guide to methods and applications. San Diego (CA): Academic Press; 1990. p. 315-22.
  13. Ordonez NF, Tupac Otero J, Diez GM. Orchid endophytes and their effect on growth in Vanilla planifolia Andrews. Acta Agron 2012;61:282-90.
  14. Vujanovic V, St-Arnaud M, Barabe D, Thibeault G. Viability testing of orchid seed and the promotion of colouration and germination. Ann Bot 2000;86:79-86. https://doi.org/10.1006/anbo.2000.1162
  15. Zelmer CD. Interactions between northern terrestrial orchids and fungi in nature [thesis]. Edmonton: University of Alberta; 1994.