Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Behavior, Survival and Blood Physiological Responses of Red-spotted Grouper Epinephelus akaara, at Different Water Temperature

수온별 붉바리(Epinephelus akaara)의 행동, 생존율 및 혈액생리학적 반응

  • Park, Hyung-Jun (Aquaculture Research Division, National Institute of Fisheries Science) ;
  • Min, Byung Hwa (Aquaculture Research Division, National Institute of Fisheries Science) ;
  • Kim, Sung-Yeon (Aquaculture Research Division, National Institute of Fisheries Science)
  • 박형준 (국립수산과학원 양식관리과) ;
  • 민병화 (국립수산과학원 양식관리과) ;
  • 김성연 (국립수산과학원 양식관리과)
  • Received : 2017.03.17
  • Accepted : 2017.04.11
  • Published : 2017.06.30
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Abstract

To determine the optimum water temperature (a temperature which minimizes metabolic activities and stress in fish) for long distance transportation of red-spotted grouper (Epinephelus akaara), by evaluating the behavior, survival and physiological and hematological responses to variable water temperature conditions (9, 12, 15, 18 and $21^{\circ}C$) for 48 hours. Fish exposed to $9^{\circ}C$ died at 48 hours exposure, but those exposed to 12, 15, 18 and $21^{\circ}C$ treatment groups all survived. Fish in the 15, 18 and $21^{\circ}C$ exposure groups exhibited normal swimming, while those exposed to $12^{\circ}C$ were observed to be stationary at the bottom of the tank. The plasma cortisol and glucose concentration were higher in fish exposed to $12^{\circ}C$ than at other temperature conditions. The fish in the 12 and $15^{\circ}C$ groups had significantly higher hematocrit (Ht) and hemoglobin (Hb) than those in the 18 and $21^{\circ}C$ groups (P<0.05). Levels of aspartate aminotransferase (AST), $NH_3$ and osmolality showed a pattern similar to the levels of plasma cortisol, while alanine aminotransferase (ALT) and total protein did not significantly vary among the experimental groups. These results indicate that the optimum water temperature for long distance transportation of the red-spotted grouper is $15^{\circ}C$.

본 연구에서는 붉바리(Epinephelus akaara)의 장거리 수송 시 최적의 수온 (대사활동과 스트레스를 최소화하는 수온)을 조사하고자, 수온별(9, 12, 15, 18 및 $21^{\circ}C$)로 48시간 동안 어체를 노출시켜 어류의 행동 변화, 생존 및 혈액생리학적 반응을 확인하였다. $9^{\circ}C$에 노출된 붉바리는 48시간만에 전량 폐사하였지만, 12 15, 18 및 $21^{\circ}C$의 그룹에서는 모두 생존하였다. 15, 18 및 $21^{\circ}C$의 붉바리는 정상적인 유영활동을 보였으나, $12^{\circ}C$에 노출시킨 붉바리는 유영없이 수조 바닥에 가라앉아 있는 상태를 보였다. 혈장 코티졸 농도와 글루코스는 다른 실험구보다 $12^{\circ}C$에 노출시킨 붉바리에서 더 유의하게 높았다(P<0.05). 12 및 $15^{\circ}C$ 그룹은 18 및 $21^{\circ}C$보다 Ht 및 Hb가 유의한 차이를 보였다(P<0.05). AST, $NH_3$ 및 삼투질 농도는 혈장 코티졸과 비슷한 양상을 보였으나(P<0.05), ALT와 총단백질은 다른 실험구에 비해 유의적인 차이를 보이지 않았다(P>0.05). 이러한 결과로 미루어 볼 때, 붉바리의 장거리 수송을 위한 적정 수온은 $15^{\circ}C$ 임을 시사하고 있다.

Keywords

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