대한한의학회지 (The Journal of Korean Medicine)

  • 제38권2호
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  • Pages.53-60
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  • 2017
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  • 1010-0695(pISSN)
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  • 2288-3339(eISSN)
대한한의학회 (The Society of Korean Medicine)
권호 표지
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Beakdugu-tang, Traditional Korean Digestant Medicine, Inhibits Hepatic Steatosis in Insulin Resistance Cell Model with HepG2 and THP-1

  • Kim, Hyuck (Department of Diagnostics, College of Korean Medicine, Dongguk University) ;
  • Lim, Dong-Woo (Department of Pathology, College of Korean Medicine, Dongguk University) ;
  • Park, Sung Yun (Department of Diagnostics, College of Korean Medicine, Dongguk University) ;
  • Park, Sun-Dong (Department of Prescription, College of Korean Medicine, Dongguk University) ;
  • Park, Won-Hwan (Department of Diagnostics, College of Korean Medicine, Dongguk University) ;
  • Kim, Jai-Eun (Department of Pathology, College of Korean Medicine, Dongguk University)
  • 투고 : 2017.05.26
  • 심사 : 2017.06.21
  • 발행 : 2017.06.30
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초록

Objectives: Beakdugu-tang (BDGT) consists of three medicinal herbs, and this prescription has long been used in treatment of various digestant problem in Korea. In this study, we designed to clarify mechanisms by which Korean traditional digestive medicine, BDGT, may exert anti-hepatic steatosis effects via improved insulin resistance cell model in human hepatocellular carcinoma (HepG2) and monocyte (THP-1). Materials and methods: The preparation of BDGT and constituents were extracted with 70% ethanol. HepG2 and THP-1 were treated with different concentrations of BDGT and constituents in the presence and absence of stimulants such as free fatty acids (FFAs) and oxidized low-density lipoprotein (ox-LDL), respectively. Results: The BDGT and its constituents inhibited the FFAs-stimulated lipid accumulation in HepG2 cells. Ethanol extracts of Amomum cardamomum (ACE) improved the ox-LDL induced insulin resistance in THP-1 cells. Also, treatment of monocytic cells with ACE increased anti-hepatic steatosis related gene levels including ABCA, ABCG and SR-B1. Conclusion: The results suggest that the ethanol extract of BDGT and its constituents potently inhibit the FFAs- and ox-LDL induced liver steatosis via improved insulin resistance.

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참고문헌

  1. Richard J, Lingvay I. Hepatic steatosis and type 2 diabetes: current and future treatment considerations. Expert Rev Cardiovasc Ther. 2011;9(3):321-8. https://doi.org/10.1586/erc.11.15
  2. Firneisz G. Non-alcoholic fatty liver disease and type 2 diabetes mellitus: the liver disease of our age?. World J Gastroenterol. 2014;20(27):9072-89. https://doi.org/10.3748/wjg.v20.i27.9072
  3. Lee DH, Han JM, Yang WM. The effects of Atractylodes japonica Koidz. on type 2 diabetic rats. J Korean Med. 2015;35(1):75-85.
  4. Weyer C, Funahashi T, Tanaka S, Hotta K, Matsuzawa Y, Prattey RE, et al. Hypoadiponectinemia in obesity and type 2 diabetes: close association with insulin resistance and hyperinsulinemia. J Clin Endocrinol Metab. 2001;86(5):1930-5. https://doi.org/10.1210/jcem.86.5.7463
  5. Kumashiro N, Erion DM, Zhang D, Kahn M, Beddow SA, Chu X, et al. Cellular mechanism of insulin resistance in nonalcoholic fatty liver disease. Proc Natl Acad Sci USA. 2011;108(39):16381-5. https://doi.org/10.1073/pnas.1113359108
  6. Hundal RS, Krssak M, Dufour S, Laurent D, Lebon V, Chandramouli V, et al. Mechanism by which metformin reduces glucose production in type 2 diabetes. Diabetes. 2000;49(12):2063-9. https://doi.org/10.2337/diabetes.49.12.2063
  7. Mitchell PL, Nachbar R, Lachance D, St-Pierre P, Trottier J, Barbier O, et al. Treatment with a novel agent combining docosahexaenoate and metformin increase protectin DX and IL-6 production in skeletal muscle and reduces insulin resistance in obese diabetic db/db mice. Diabetes Obes Metab. 2017;19(3):313-9. https://doi.org/10.1111/dom.12818
  8. Diamanti-Kandarakis E, Kouli C, Tsianateli T, Bergiele A. Therapeutic effects of metformin on insulin resistance and hyperandrogenism in polycystic ovary syndrome. Eur J Endocrinol. 1998;138(3):269-74. https://doi.org/10.1530/eje.0.1380269
  9. Kwon OJ, Lee SW, Paik SH, Han SR, Ahn YM, Ahn SY, et al. The effects of Platycodi radix on obese type 2 diabetes mouse model induced by high fat, high carbohydrate diet. J Korean Med. 2013;34(1):1-14.
  10. Lim DW, Bose S, Wang JH, Choi HS, Kim YM, Chin YW, et al. Modified SJH alleviates FFAs-induced hepatic steatosis through leptin signaling pathways. Sci Rep. 2017;30(7):45425.
  11. Lim DW, Kim H, Park JY, Kim JE, Moon JY, Park SD, et al. Amomum cardamomum L. ethyl acetate fraction protects against carbon tetrachloride -induced liver injury via an antioxidant mechanism in rats. BMC Complement Altern Med. 2016;16:155. https://doi.org/10.1186/s12906-016-1121-1
  12. Kobayashi Y, Hara N, Sugimoto R, Mifuji -Moroka R, Tanaka H, Eguchi A, et al. The associations between circulating bile acids and the muscle volume in patients with non-alcoholic fatty liver disease (NAFLD). Intern Med. 2017; 56(7):755-62. https://doi.org/10.2169/internalmedicine.56.7796
  13. Lee KP, Kim JE, Kim H, Chang HR, Lee DW, Park WH. Bo-Gan-Whan regulates proliferation and migration of vascular smooth muscle cells. BMC Complement Altern Med. 2016;16:306. https://doi.org/10.1186/s12906-016-1292-9
  14. Um ES, Kim YC. Effect of samhwangsasim-tang and daehwanghwangryunsasim-tang on palmitate-induced lipogenesis in HepG2 cells. J Korean Med. 2016;37(1):62-76. https://doi.org/10.13048/jkm.16007
  15. Heo J. Translated Dongeuibogam. 1st ed. Seoul: Bubinmunwha. 1999.
  16. Ulla A, Alam MA, Sikder B, Sumi FA, Rahman MM, Habib ZF, et al. Supplementation of Syzygium cumini seed powder prevented obesity, glucose intolerance, hyperlipidemia and oxidative stress in high carbohydrate high fat diet induced obese rats. BMC Complement Altern Med. 2017;17:289 https://doi.org/10.1186/s12906-017-1799-8
  17. Huang Q, Wang T, Yang L, Wang HY. Gisenosied Rb2 alleviates hepatic lipid accumulation by resorting autophagy via induction of Sirt1 and activation of AMPK. Int J Mol Sci. 2017; 19(18):E1063.
  18. Zhang M, Liu C, Hu MY, Zhang J, Xu P, Li F, et al. High-fat diet enhanced retinal dehydrogenase activity, but suppressed retinol dehydrogenase activity in liver of rats. J Pharmacol Sci. 2015;127(4):430-438. https://doi.org/10.1016/j.jphs.2015.03.001
  19. Gotoh K, Masaki T, Chiba S, Ando H, Fujiwara K, Shimasaki T, et al. Effects of hydrophilic statins on renal tubular lipid accumulation in diet-induced obese mice. Obes Res Clin Pract. 2013;7(5):e342-352. https://doi.org/10.1016/j.orcp.2013.04.001
  20. Bonora E, Targher G. Increase risk of cardiovascular disease and chronic kidney disease in NAFLD. Nat Rev Gastroenterol Hepatol. 2012;9(7):372-381. https://doi.org/10.1038/nrgastro.2012.79
  21. Masarone M, Rosato V, Aglitti A, Bucci T, Caruso R, Salvatore T, et al. Liver biopsy in type 2 diabetes mellitus: Steatohepatitis represents the sole feature of liver damage. PLOS One. 2017;12(6):e0178473. https://doi.org/10.1371/journal.pone.0178473
  22. Bastard JP, Maachi M, Lagath C, Kim MJ, Caron M, Vidal H, et al. Recent advances in the relationship between obesity, inflammation, and insulin resistance. Eur Cytokine Netw. 2006; 17(1):4-12.