Clinical and Experimental Reproductive Medicine

  • 제44권1호
  • /
  • Pages.52-55
  • /
  • 2017
  • /
  • 2233-8233(pISSN)
  • /
  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
권호 표지
DOI QR코드

DOI QR Code

A successful pregnancy using completely immotile but viable frozen-thawed spermatozoa selected by laser

  • Chen, Huanhua (Center for Reproductive Medicine, The Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region) ;
  • Feng, Guixue (Center for Reproductive Medicine, The Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region) ;
  • Zhang, Bo (Center for Reproductive Medicine, The Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region) ;
  • Zhou, Hong (Center for Reproductive Medicine, The Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region) ;
  • Shu, Jinhui (Center for Reproductive Medicine, The Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region) ;
  • Gan, Xianyou (Center for Reproductive Medicine, The Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region)
  • 투고 : 2016.10.20
  • 심사 : 2017.01.19
  • 발행 : 2017.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The aim of this study was to report a successful pregnancy using completely immotile frozen-thawed spermatozoa selected by laser. A single laser shot was used to detect the presence of viable immotile spermatozoa in fresh and frozen-thawed testicular spermatozoa. The viability rate was 55.8% after the laser detection, and cryopreservation was carried out immediately. The thawing test was performed on the day of oocyte pick-up, and no motile sperm were observed after extending the culture for another 4 hours, while a survival rate of 39.8% was detected using the laser. In all, five mature oocytes were injected, resulting in four cases of normal fertilization (80%) on day 1. Further, two high-quality day 3 embryos were transferred, which resulted in a singleton pregnancy. Our study demonstrates that completely immotile spermatozoa are worth cryopreserving for further intracytoplasmic sperm injection, which provides a new insight into male fertility preservation in cases of completely immotile spermatozoa.

키워드

참고문헌

  1. Hessel M, Robben JC, D'Hauwers KW, Braat DD, Ramos L. The influence of sperm motility and cryopreservation on the treatment outcome after intracytoplasmic sperm injection following testicular sperm extraction. Acta Obstet Gynecol Scand 2015;94: 1313-21. https://doi.org/10.1111/aogs.12769
  2. Stalf T, Mehnert C, Hajimohammad A, Manolopoulos K, Shen Y, Schuppe HC, et al. Influence of motility and vitality in intracytoplasmic sperm injection with ejaculated and testicular sperm. Andrologia 2005;37:125-30. https://doi.org/10.1111/j.1439-0272.2005.00665.x
  3. Javed M, Esfandiari N, Casper RF. Failed fertilization after clinical intracytoplasmic sperm injection. Reprod Biomed Online 2010; 20:56-67. https://doi.org/10.1016/j.rbmo.2009.10.010
  4. John Morris G, Acton E, Murray BJ, Fonseca F. Freezing injury: the special case of the sperm cell. Cryobiology 2012;64:71-80. https://doi.org/10.1016/j.cryobiol.2011.12.002
  5. World Health Organization. WHO laboratory manual for the examination and processing of human semen. 5th ed. Geneva: World Health Organization; 2010.
  6. Feng G, Zhang B, Zhou H, Shu J, Gan X, Wu F, et al. Comparable clinical outcomes and live births after single vitrified-warmed and fresh blastocyst transfer. Reprod Biomed Online 2012;25: 466-73. https://doi.org/10.1016/j.rbmo.2012.07.008
  7. Aktan TM, Montag M, Duman S, Gorkemli H, Rink K, Yurdakul T. Use of a laser to detect viable but immotile spermatozoa. Andrologia 2004;36:366-9. https://doi.org/10.1111/j.1439-0272.2004.00636.x
  8. Desch L, Bruno C, Herbemont C, Michel F, Bechoua S, Girod S, et al. Impact on ICSI outcomes of adding 24 h of in vitro culture before testicular sperm freezing: a retrospective study. Basic Clin Androl 2015;25:6. https://doi.org/10.1186/s12610-015-0022-3
  9. Gerber PA, Kruse R, Hirchenhain J, Krussel JS, Neumann NJ. Pregnancy after laser-assisted selection of viable spermatozoa before intracytoplasmatic sperm injection in a couple with male primary cilia dyskinesia. Fertil Steril 2008;89:1826.e9-12.
  10. Geber S, Lemgruber M, Taitson PF, Valle M, Sampaio M. Birth of healthy twins after intracytoplasmic sperm injection using ejaculated immotile spermatozoa from a patient with Kartagener's syndrome. Andrologia 2012;44 Suppl 1:842-4. https://doi.org/10.1111/j.1439-0272.2011.01224.x
  11. Montjean D, Courageot J, Altie A, Amar-Hoffet A, Rossin B, Geoffroy-Siraudin C, et al. Normal live birth after vitrified/warmed oocytes intracytoplasmic sperm injection with immotile spermatozoa in a patient with Kartagener's syndrome. Andrologia 2015;47:839-45. https://doi.org/10.1111/and.12331
  12. Nordhoff V, Schuring AN, Krallmann C, Zitzmann M, Schlatt S, Kiesel L, et al. Optimizing TESE-ICSI by laser-assisted selection of immotile spermatozoa and polarization microscopy for selection of oocytes. Andrology 2013;1:67-74. https://doi.org/10.1111/j.2047-2927.2012.00020.x
  13. Ortega C, Verheyen G, Raick D, Camus M, Devroey P, Tournaye H. Absolute asthenozoospermia and ICSI: what are the options? Hum Reprod Update 2011;17:684-92. https://doi.org/10.1093/humupd/dmr018
  14. Nordhoff V. How to select immotile but viable spermatozoa on the day of intracytoplasmic sperm injection? An embryologist's view. Andrology 2015;3:156-62. https://doi.org/10.1111/andr.286
  15. Buckett WM. Predictive value of hypo-osmotic swelling test to identify viable non-motile sperm. Asian J Androl 2003;5:209-12.
  16. Tubman A, Check JH, Bollendorf A, Wilson C. Effect of poor motility on pregnancy outcome following intracytoplasmic sperm injection in couples whose male partners have subnormal hypoosmotic swelling test scores. Clin Exp Obstet Gynecol 2013;40:315-6.
  17. Hossain A, Osuamkpe C, Hossain S, Phelps JY. Spontaneously developed tail swellings (SDTS) influence the accuracy of the hypo-osmotic swelling test (HOS-test) in determining membrane integrity and viability of human spermatozoa. J Assist Reprod Genet 2010;27:83-6. https://doi.org/10.1007/s10815-009-9375-x
  18. Ebner T, Moser M, Yaman C, Sommergruber M, Tews G. Successful birth after laser assisted immobilization of spermatozoa before intracytoplasmic injection. Fertil Steril 2002;78:417-8. https://doi.org/10.1016/S0015-0282(02)03208-9
  19. Ebner T, Yaman C, Moser M, Sommergruber M, Hartl J, Tews G. Laser assisted immobilization of spermatozoa prior to intracytoplasmic sperm injection in humans. Hum Reprod 2001;16:2628-31. https://doi.org/10.1093/humrep/16.12.2628
  20. Chen HH, Feng GX, Zhang B, Shu JH, Gan XY, Zhou H, et al. Successful pregnancy following laser-assisted selection of viable but immotile spermatozoa for intracytoplasmic sperm injection: a report of 2 cases. Zhonghua Nan Ke Xue 2015;21:988-91.

피인용 문헌

  1. Comparison of intracytoplasmic sperm injection outcome with fresh versus frozen-thawed testicular sperm in men with nonobstructive azoospermia: a systematic review and meta-analysis vol.35, pp.7, 2017, https://doi.org/10.1007/s10815-018-1206-5
  2. Sperm selection strategies and their impact on assisted reproductive technology outcomes vol.53, pp.2, 2017, https://doi.org/10.1111/and.13725
  3. Sperm Selection Procedures for Optimizing the Outcome of ICSI in Patients with NOA vol.10, pp.12, 2017, https://doi.org/10.3390/jcm10122687
  4. Diagnostics and Management of Male Infertility in Primary Ciliary Dyskinesia vol.11, pp.9, 2017, https://doi.org/10.3390/diagnostics11091550