Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Phytochemicals and antioxidant activity in the kenaf plant (Hibiscus cannabinus L.)

  • Ryu, Jaihyunk (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kwon, Soon-Jae (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Ahn, Joon-Woo (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Jo, Yeong Deuk (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Sang Hoon (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Jeong, Sang Wook (Jangheung Research Institute for Mushroom Industry) ;
  • Lee, Min Kyu (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Jin-Baek (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kang, Si-Yong (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • Received : 2017.05.29
  • Accepted : 2017.06.20
  • Published : 2017.06.30
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Abstract

Chemical compounds from four different tissues of the kenaf plant (Hibiscus cannabinus), a valuable medicinal crop originating from Africa, were examined to determine its potential for use as a new drug material. Leaves, bark, flowers, and seeds were harvested to identify phytochemical compounds and measure antioxidant activities. Gas chromatography mass spectrometry analyses identified 22 different phytocompounds in hexane extracts of the different parts of the kenaf plant. The most abundant volatile compounds were E-phytol (32.4%), linolenic acid (47.3%), trisiloxane-1,1,1,5,5,5-hexamethyl-3,3-bis[(trimethylsilyl)oxy] (16.4%), and linoleic acid (46.4%) in leaves, bark, flowers, and seeds, respectively. Ultra-high performance liquid chromatography identified the major compounds in the different parts of the kenaf plant as kaemperitrin, caffeic acid, myricetin glycoside, and p-hydroxybenzoic acid in leaves, bark, flowers, and seeds, respectively. Water extracts of flowers, leaves, and seeds exhibited the greatest DPPH radical scavenging activity and SOD activity. Our analyses suggest that water is the optimal solvent, as it extracted the greatest quantity of functional compounds with the highest levels of antioxidant activity. These results provide valuable information for the development of environmentally friendly natural products for the pharmaceutical industry.

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References

  1. Abegunde SM, Ayodele-Oduola RO (2013) Comparison of efficiency of different solvents used for the extraction of phytochemicals from the leaf, seed and stem bark of Calotropis procera. IJSR.4: 835-838
  2. Alexopoulou E, Papattheohari Y, Christou M, Monti A (2013) Keanf: A multi-purpose crop for sever industrial applications. Springer-verlag, London. pp.1-15
  3. Chen KW, Iqbal S, Khong NMH, Ooi DJ, Ismail M (2014) Antioxidant activity of phenolicsesaponins rich fraction prepared from defatted kenaf seed meal. LWT-Food Sci. Techno. 56:181-186 https://doi.org/10.1016/j.lwt.2013.10.028
  4. De Freitas RB, Rovani BT, Boligon AA, de Brum TF, Piana M, da Silva Jesus R, Veloso CF, Kober H, Moresco RN, da Costa Araldi IC, de Freitas Bauermann ICL, Athayde ML (2013) Hepatotoxicity evaluation of aqueous extract from Scutia buxifolia. Molecules18:7570-7583 https://doi.org/10.3390/molecules18077570
  5. Ebije I, Oladipupo A, Lawal AOO, Isiaka AO (2014) Volatile composition of the floral essential oil of Hibiscus sabdariffa L.from Nigeria. AJEONP. 2:04-07
  6. Ghafar SAA, Ismail M, Yazan LS, Fakurazi S, Ismail N, Chan KW, Tahir PM (2013) Cytotoxic activity of kenaf seed oils from supercritical carbon dioxide fluid extraction towards human colorectal cancer (HT29) cell lines. Evid. Based Complement. Alternat. Med. 2013:549705
  7. Ghafar SAA, Yazan LS, Tahir PM, Ismail M (2012) Kenaf seed supercritical fluid extract reduces aberrant crypt foci formation in azoxymethane-induced rats. Exp.Toxic. Pathol.16:247-251
  8. Hossain MD, Hanafi MM, Jol H, Jamal T (2011) Dry matter and nutrient partitioning of kenaf (Hibiscus cannabinus L.) varieties grown on sandy bris soil. Aust. J. Crop Sci. 5(6):654-659
  9. Inoue Y, Hada T, Shiraishi A, Hirore K, Hamashima H, Kobayashi S (2005) Biphasic effects of geranylgeraniol, teprenone, and phytol on the growth of Staphylococcus aureus. Antimicrob.agents Chemother.49:1770-1774 https://doi.org/10.1128/AAC.49.5.1770-1774.2005
  10. Jin CW, Ghimeray AK, Wang L, Xu ML, Piao JP, Cho DH (2013) Far infrared assisted kenaf leaf tea preparation and its effect on phenolic compounds, antioxidant and ACE inhibitory activity. 2013. J.Med.Plants Res.7:1121-1128
  11. Jorge AP, Horst H, Sousa E, Pizzolatti MG, Silva FRMB (2004) Insulinomimetic effects of kaempferitrin on glycaemia and on $^{14}C$ glucose uptake in rat soleus muscle. Chem.Biol.Inter.149: 89-96 https://doi.org/10.1016/j.cbi.2004.07.001
  12. Khare CP (2007) Indian medicinal plants; An illustrated dictionary. Springer-verlag, London. p. 309
  13. Kobaisy M, Tellez MR, Webber CL, Dayan FE, Schrader KK, Wedge DE (2001) Phytotoxic and fungitoxic activities of the essential oil of kenaf (Hibiscus cannabinus L.) leaves and its composition. J.Agric.Food Chem.49:3768-3771 https://doi.org/10.1021/jf0101455
  14. Kubmarawa D, Andenyang IFH, Magomya AM (2009) Proximate composition and amino acid profile of two non-conventional leafy vegetables (Hibiscus cannabinus and Haematostaphis barteri). Afr.J.Food Sci. 3:233-236
  15. Mohamed A, Bhardwaj H, Hamama A, Webber C (1995) Chemical composition of kenaf (Hibiscus cannabinus L.)seed oil. Ind. Crop Prod. 4:157-165 https://doi.org/10.1016/0926-6690(95)00027-A
  16. Nandagopalan V, Johnson Gritto M, Doss A (2015) GC-MS analysis of bioactive components of the methanol extract of Hibiscus tiliaceus Linn. Asian J. Plant Sci. Res. 5:6-10
  17. Nyam KL, Tan CP, Lai OM, Long K, Che Man YB (2009) Physicochemical properties and bioactive compounds of selected seed oils. Food Sci.Techol. 42:1396-1403
  18. Obouayeba AP, Diarrassouba M, Soumahin EF, Kouakou TH (2015) Phytochemical analysis, purification and identification of Hibiscus anthocyanins. J.Pharm.Chem.Biol.Sci. 3:156-168
  19. Rakhimkhanov ZB, Sadykov AS, Ismailov AI, Karimdzhanov AK (1970) A study of the anthocyanins of kenaf. Khimiya Prirodnykh Soedinenii. 6:129-130
  20. Rauter A, Palma FB, Justino J, Araujo ME, Santos SP (2002) Natural Products in the New Millennium: Prospects and Industrial Application. Kluwer academic publishers, Dordreche, Netherlands. pp. 47-57
  21. Ryu J, Ha BK, Kim DS, Kim JB, Kim SH, Kang SY (2013) Assessment of growth and seed oil composition of kenaf (Hibiscus cannabinus L.) germplasm. J.Crop Sci. Biotech. 16: 297-302 https://doi.org/10.1007/s12892-013-0074-x
  22. Ryu, J, Kwon SJ, Ahn JW, Ha BK, Jeong SW, Im SB, Kim JB, Kim SH, Lee YK, Kang SY (2016) Evaluation of nutritive value and identification of fungi in silage from new kenaf (Hibiscus cannabinus) cultivars. Int.J.Agric.Biol.18:1159-1168 https://doi.org/10.17957/IJAB/15.0220
  23. Salem MZM, Olivares-Perez J, Salem AZM (2014) Studies on biological activities and phytochemicals composition of Hibiscus species-A review. Life Sci. J. 11:1-8
  24. Shaheen MA, El-Nakhlawy FS, Al-Shareef AR (2012). Roselle (Hibiscus sabdariffa L.) seeds as unconventional nutritional source. Afr.J.Biotech.11:9821-9824
  25. Sultana B, Anwar F, Przybylski R (2007) Antioxidant activity of phenolic components present in barks of Azadirachta indica, Terminalia arjuna, Acacia nilotica, and Eugenia jambolana Lam.Trees. Food Chem.104:1106-1114 https://doi.org/10.1016/j.foodchem.2007.01.019
  26. Wang J, Sun B, Cao Y, Tian Y, Li X (2008) Optimisation of ultrasound-assisted extraction of phenolic compounds from wheat bran. Food Chem.106:804-810 https://doi.org/10.1016/j.foodchem.2007.06.062
  27. Yusri NM, Chen KW, Iqbal S, Ismail M (2012) Phenolic content and antioxidant activity of Hibiscus cannabinus L. seed extracts after sequential solvent extraction. Molecules17:12612-12621 https://doi.org/10.3390/molecules171112612
  28. Zhao S, Li X, Cho DH, Arasu MV, Al-Dhabi NA, Park SU (2014) Accumlation of kaempferitrin and expression of phenyl-propanoid biosynthetic genes in kenaf (Hibiscus cannabinus). Molecules 19:16987-16997 https://doi.org/10.3390/molecules191016987
  29. Zhishen J, Mengcheng T, Jianming W (1999) The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 64:555-559 https://doi.org/10.1016/S0308-8146(98)00102-2

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