Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Development of an efficient protocol for high-frequency regeneration system in Hibiscus syriacus L.

  • Seo, Sang-Gyu (Department of Environmental Horticulture, The University of Seoul) ;
  • Ryu, Sun-Hee (Department of Environmental Horticulture, The University of Seoul) ;
  • Zhou, Yang (Department of Environmental Horticulture, The University of Seoul) ;
  • Kim, Sun-Hyung (Department of Environmental Horticulture, The University of Seoul)
  • Received : 2017.06.21
  • Accepted : 2017.06.21
  • Published : 2017.06.30
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Abstract

A rapid and efficient in vitro regeneration system was established for Hibiscus syriacus L. The successful regeneration protocol employs induction of shoot organogenesis on leaf, petiole, and root explants. Among the various plant growth regulators evaluated, thidiazuron (TDZ) was the most effective for inducing rapid shoot formation. Most efficient shoot regeneration frequency was obtained from Murashige and Skoog (MS) media containing 0.01 mg/L TDZ. Regeneration efficiency was highest in the roots, and lowest in the leaves. A combination of 0.01 mg/L TDZ with benzyladenine (BAP) markedly improved the frequency of shoot differentiation from the root (up to 98%) and petiole (up to 88%) explants. Furthermore, leaf and petiole explants showed the highest frequency of shoot induction in half-strength MS media containing 0.01 mg/L TDZ and 1.0 mg/L BAP, while root explants formed the greatest number of shoots when 0.01 mg/L TDZ and 0.1 mg/L BAP were added to half-strength MS media. Although the frequency of shoot differentiation from leaf explants was only 50%, the leaf is considered the most efficient plant organ for use in tissue culture because leaves are easier to obtain than roots and petioles. Our findings show that various organs of H. syriacus can be used for plant regeneration, and the protocol developed in this study may be applicable in the horticulture industry.

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References

  1. Bates S, Navarrete JE, Sammbeek JW, Gaffney GR (1992) Thidiazuron stimulates shoot organogenesis and somatic embryogenesis in white ash (Fraxinus americana L). Plant Cell Tissue Organ Cult 32:21-29
  2. Cui J, Chen JJ, Henny RJ (2009) Regeneration of Aeschynanthusradicans via direct somatic embryogenesis and analysis of regenerants with flow cytometry. In Vitro Cell Dev Biol Plant 45:34-43
  3. Fellman CD, Read PE, Hosier MA (1987) Effects of thidiazuron and CPPU on meristem formation and shoot proliferation. HortScience 22:1197-1200
  4. Govinden-Soulange J, Boodia N, Dussooa C, Gunowa R, Deensah S, Facknath S, Rajkomar B (2009) Vegetative propagation and tissue culture regeneration of Hibiscus sabdariffa L(Roselle). World J Agric Sci 5:651-661
  5. Guo B, Abbasi BH, Zeb A, Xu LL, Wei YH (2011) Thidiazuron: A multi-dimensional plant growth regulator. Afr J Biotechnol 10:8984-9000 https://doi.org/10.5897/AJB11.636
  6. Hartman BH, Kester DE, Davies FT (1994) Plant propagation: principles and techniques. p. 246-247. Prentice Hall, New Jersey.
  7. Hotta M, Ogata K, Nitta A, Hosikawa K, Yanagi M,Yamazaki K (1989) Useful plants of the world. p. 1499. Heibonsha, Tokyo, Japan
  8. Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Organ Cult 33:105-119 https://doi.org/10.1007/BF01983223
  9. Hutchinson MJ, Murch SJ, Saxena PK (1996) Morphoregulatory role of thidiazur on: evidence of the involvement of endogenous auxin in thidiazuron-induced somatic embryogenesis of geranium (Pelargonium x hortorum Bailey). J plant physiol 149:573-579 https://doi.org/10.1016/S0176-1617(96)80336-1
  10. Jeon SB, Kang SW, Kim WS, Lee GP, Kim SH, Seo SG (2009) In vitro plant regeneration from axillary buds of Hibiscus syriacus L. J Plant Biotechnol 36:174-178 https://doi.org/10.5010/JPB.2009.36.2.174
  11. Jones MPA, Cao J, O'Brien R, Murch SJ, Saxena PK (2007) The mode of action of thidiazuron: auxins, indoleamines, and ion channels in the regeneration of Echinacea purpurea L. Plant Cell Rep 26:1481-1490 https://doi.org/10.1007/s00299-007-0357-0
  12. Kim EK, Yoo YK, Kim KS (1998) Effect of thidiazuron on callus and multiple shoot formation in shoot-tip culture of Hibiscus syriacus L. 'Honghwarang'. Kor J Hort Sci Technol 16:520-524
  13. Kumari, S. and R.K. Pandey (2011) In vitro plant regeneration form shoot tip explants of Hibiscus syriacus (L.). Int Q J Life Sci 6:647-648
  14. Lo KH, Giles KL, Sawhney VK (1997) Acquisition of competence for shoot regeneration in leaf discs of Saintpaulia ionantha x confusa hybrids (African violet) cultured in vitro. Plant Cell Rep 16: 416-420
  15. Ma GH, Lu J, Teixeira da Silva JA, Zhang X, Zhao J (2011) Shoot organogenesis and somatic embryogenesis from leaf and shoot explants of Ochna integerrima (Lour). Plant Cell Tissue Organ Cult 104:157-162 https://doi.org/10.1007/s11240-010-9812-7
  16. Mithila J, Hall JC, Victor JMR, Saxena PK (2003) Thidiazuron induces shoot organogenesis at low concentrations and somatic embryogenesis at high concentrations on leaf and petiole explants of African violet (Saintpaulia Ionantha Wendl.). Plant Cell Rep 21:408-414 https://doi.org/10.1007/s00299-002-0544-y
  17. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  18. Murch SJ, Saxena PK (2001) Molecular fate of thidiazuron and its effects on auxin transport in hypocotyls tissues of Pelargonium x hortorum Bailey. Plant Growth Regul 35:269-275 https://doi.org/10.1023/A:1014468905953
  19. Murthy BNS, Murch SJ, Saxena PK (1998) Thidiazuron: A potent regulator ofin vitro plant morphogenesis. In Vitro Cellular & Developmental Biology-Plant 34:267 https://doi.org/10.1007/BF02822732
  20. Neuman MC, Preece JE, Gaffney GR, VANSAMBEEK JW (1988) Production of embryoid-like structures from immature cotyledonary tissue of black walnut (Juglansnigra L). HortScience23:807
  21. Peak KY, Son YJ, Hwang JK, Jong SK, Park SI (1989) Plant regeneration in tissue culture initiated from immature embryos of Hibiscus syriacus L. Korean J Plant Tissue Cult 16:93-104
  22. Taha RM, Daud N, Hasbullah NA, Awal A (2009) Somatic embryogenesis and production of artificial seeds in Saintapaulia ionantha Wendl. Acta Hortic 829:331-336
  23. Takagi H, Sugawara S, Saito T, Tasaki H, Yuanxue L, Kaiyun G, Han D-S, Godo T, Nakano M (2011) Plant regeneration via direct and indirect adventitious shoot formation and chromosomedoubled somaclonal variation in Titanotrichum oldhamii (Hemsl.) Solereder. Plant Biotechnol Rep 5:187-195 https://doi.org/10.1007/s11816-011-0172-5
  24. Tiwari V, Tiwari KN, Singh BD (2001) Comparative studies of cytokinins on in vitro propagation of Bacopamonniera. Plant Cell Tissue Organ Cult 66:9-16 https://doi.org/10.1023/A:1010652006417
  25. Visser C, Qureshi JA, Gill R, Saxena PK (1992) Morphoregulatory role of TDZ. Substitution of auxin and cytokinin requirement for the induction of somatic embryogenesis in geranium hypocotyl culture. Plant Physiol 99:1704-1707 https://doi.org/10.1104/pp.99.4.1704
  26. Yoo EH, YooYK, Kim KS (1996) Effects of growth regulators, sucrose, light, and medium on callus and shoot formation in shoot-tip culture of Hibiscus syriacus L. 'Honghwarang'. J Kor Soc Hort Sci 37:317-323
  27. Zhao YX, Yao DY, Harris PJC (1991) Isolation and culture of protoplasts from callus tissue of Hibiscus syriacus L. Plant Cell Tissue Organ Cult 25:17-19 https://doi.org/10.1007/BF00033907