Journal of Plant Biotechnology

  • 제44권2호
  • /
  • Pages.135-141
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  • 2017
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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구지뽕 나무의 엽록체 TrnL-F 영역 염기서열 분석을 통한 특이적 SNP 분자마커의 확인

Identification of specific SNP molecular marker from Cudrania tricuspidata using DNA sequences of chloroplast TrnL-F region

  • 이수진 (국립경남과학기술대학교 생명과학대학 농학.한약자원학부) ;
  • 신용욱 (국립경남과학기술대학교 생명과학대학 농학.한약자원학부) ;
  • 김윤희 (국립경상대학교 사범대학 생물교육과 (농업생명과학연구원)) ;
  • 이신우 (국립경남과학기술대학교 생명과학대학 농학.한약자원학부)
  • Lee, Soo Jin (Department of Agronomy & Medicinal Plant Resources, Gyeongnam National University of Science & Technology) ;
  • Shin, Yong-Wook (Department of Agronomy & Medicinal Plant Resources, Gyeongnam National University of Science & Technology) ;
  • Kim, Yun-Hee (Department of Biology Education, College of Education, IALS, Gyeongsang National University) ;
  • Lee, Shin-Woo (Department of Agronomy & Medicinal Plant Resources, Gyeongnam National University of Science & Technology)
  • 투고 : 2017.05.31
  • 심사 : 2017.06.26
  • 발행 : 2017.06.30
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초록

구지뽕 나무(Cudrania tricuspidata Bureau)는 일반적으로 이용되는 대표적인 다년생의 약용식물이다. 최근 국제적 추세에 따라 자국의 유전자원의 발굴, 보존 등이 강화됨에 따라 인접국가와 국내 자생 꾸지뽕 계통을 판별 할 수 있는 기준설정에 관한 연구의 필요성이 대두되고 있지만, 분자생물학적 판별 기술의 개발은 아직 미흡한 실정이다. 본 연구에서는 국내 토종과 중국 계통의 구지뽕 나무의 기원을 판별하기 위해 엽록체에 존재하는 trnL-trnF 유전자단편에서 SNP를 이용한 판별 프라이머를 확보하였으며 이를 보완하여 보다 신속하게 판별하기 위하여 ARMS-PCR기술을 이용한 판별마커와 그 조건을 확립하였다. 그러므로, 본 연구에서 개발된 SNP 마커는 다양한 지역에서 서식하는 구지뽕 계통들의 신속한 확인을 위해 매우 유용하게 이용될 것으로 생각된다.

Cudrania tricuspidata Bureau is a widely used medicinal perennial woody plant. For conservation and germplasm utilization of the plant, it is imperative to obtaining information regarding the genetic diversity of the plant populations. Although C. tricuspidata is an important medicinal plant registered in South Korea, no molecular markers are currently available to distinguish Korean-specific ecotypes from other ecotypes of different countries. In this study, we developed single nucleotide polymorphism (SNP) markers derived from chloroplast genomic sequences to identify distinct Korean-specific ecotypes of C. tricuspidata via the amplification refractory mutation system (ARMS)-PCR analyses. Molecular authentication of twelve C. tricuspidata ecotypes from different regions was performed, using DNA sequences in the trnL-F chloroplast intergenic region. The SNP markers developed in this study are useful for rapidly identifying specific C. tricuspidata ecotypes from different regions.

키워드

참고문헌

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피인용 문헌

  1. Practical application of the Bar-HRM technology for utilization with the differentiation of the origin of specific medicinal plant species vol.45, pp.1, 2018, https://doi.org/10.5010/JPB.2018.45.1.009