Parasites, Hosts and Diseases

  • 제55권3호
  • /
  • Pages.279-285
  • /
  • 2017
  • /
  • 2982-5164(pISSN)
  • /
  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
권호 표지
DOI QR코드

DOI QR Code

Molecular Phylogenetics of Trichostrongylus Species (Nematoda: Trichostrongylidae) from Humans of Mazandaran Province, Iran

  • Sharifdini, Meysam (Department of Parasitology and Mycology, School of Medicine, Guilan University of Medical Sciences) ;
  • Heidari, Zahra (Department of Medical Microbiology, School of Medicine, Ardabil University of Medical Sciences) ;
  • Hesari, Zahra (Department of Pharmaceutics, School of Pharmacy, Guilan University of Medical Sciences) ;
  • Vatandoost, Sajad (Department of Animal Sciences, School of Biology, Faculty of Sciences, Tehran University) ;
  • Kia, Eshrat Beigom (Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences)
  • 투고 : 2016.06.21
  • 심사 : 2017.01.10
  • 발행 : 2017.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The present study was performed to analyze molecularly the phylogenetic positions of human-infecting Trichostrongylus species in Mazandaran Province, Iran, which is an endemic area for trichostrongyliasis. DNA from 7 Trichostrongylus infected stool samples were extracted by using in-house (IH) method. PCR amplification of ITS2-rDNA region was performed, and products were sequenced. Phylogenetic analysis of the nucleotide sequence data was performed using MEGA 5.0 software. Six out of 7 isolates had high similarity with Trichostrongylus colubriformis, while the other one showed high homology with Trichostrongylus axei registered in GenBank reference sequences. Intra-specific variations within isolates of T. colubriformis and T. axei amounted to 0-1.8% and 0-0.6%, respectively. Trichostrongylus species obtained in the present study were in a cluster with the relevant reference sequences from previous studies. BLAST analysis indicated that there was 100% homology among all 6 ITS2 sequences of T. colubriformis in the present study and most previously registered sequences of T. colubriformis from human, sheep, and goat isolates from Iran and also human isolates from Laos, Thailand, and France. The ITS2 sequence of T. axei exhibited 99.4% homology with the human isolate of T. axei from Thailand, sheep isolates from New Zealand and Iran, and cattle isolate from USA.

키워드

참고문헌

  1. Boreham RE, McCowan MJ, Ryan AE, Allworth AM, Robson JM. Human trichostrongyliasis in Queensland. Pathology 1995; 27: 182-185. https://doi.org/10.1080/00313029500169842
  2. Roberts LS, Schmidt GD, Janovy J. Foundations of Parasitology. 8th ed. Boston, USA. McGraw-Hill Higher Education. 2009, pp 428.
  3. Phosuk I, Intapan PM, Sanpool O, Janwan P, Thanchomnang T, Sawanyawisuth K, Morakote N, Maleewong W. Molecular evidence of Trichostrongylus colubriformis and Trichostrongylus axei infections in humans from Thailand and Lao PDR. Am J Trop Med Hyg 2013; 89: 376-379. https://doi.org/10.4269/ajtmh.13-0113
  4. Ghadirian E, Arfaa F. Present status of trichostrongyliasis in Iran. Am J Trop Med Hyg 1975; 24: 935-941. https://doi.org/10.4269/ajtmh.1975.24.935
  5. John D, Petri WA. Markell and Voge's Medical Parasitology. 9th ed. St. Louis, Missouri, USA. Elsevier. 2006, pp 266.
  6. Wall EC, Bhatnagar N, Watson J, Doherty T. An unusual case of hypereosinophilia and abdominal pain: an outbreak of Trichostrongylus imported from New Zealand. J Travel Med 2011; 18: 59-60. https://doi.org/10.1111/j.1708-8305.2010.00474.x
  7. Lattes S, Ferte H, Delaunay P, Depaquit J, Vassallo M, Vittier M, Kokcha S, Coulibaly E, Marty P. Trichostrongylus colubriformis nematode infections in humans, France. Emerg Infect Dis 2011; 17: 1301-1302. https://doi.org/10.3201/eid1707.101519
  8. Georgi JR, McCulloch CE. Diagnostic morphometry: identification of helminth eggs by discriminant analysis of morphometric data. Proc Helminthol Soc Wash 1989; 56: 44-57.
  9. Ghasemikhah R, Sharbatkhori M, Mobedi I, Kia E, Harandi MF, Mirhendi H. Sequence analysis of the second internal transcribed spacer (ITS2) region of rDNA for species identification of Trichostrongylus nematodes isolated from domestic livestock in Iran. Iran J Parasitol 2012; 7: 40-46.
  10. Hoste H, Chilton NB, Gasser RB, Beveridge I. Differences in the second internal transcribed spacer (ribosomal DNA) between five species of Trichostrongylus (Nematoda: Trichostrongylidae). Int J Parasitol 1995; 25: 75-80. https://doi.org/10.1016/0020-7519(94)00085-3
  11. Kuznetsov D, Kuznetsova N. Sequences of the second internal transcribed spacer of ribosomal DNA for three species of Trichostrongylus (Nematoda: Trichostrongylidae) from sheep in Russia. Helminthologia 2007; 44: 43-46. https://doi.org/10.2478/s11687-007-0002-z
  12. Gasser RB, Chilton NB, Hoste H, Stevenson LA. Species identification of trichostrongyle nematodes by PCR-linked RFLP. Int J Parasitol 1994; 24: 291-293. https://doi.org/10.1016/0020-7519(94)90041-8
  13. Tan TK, Panchadcharam C, Low VL, Lee SC, Ngui R, Sharma RS, Lim YA. Co-infection of Haemonchus contortus and Trichostrongylus spp. among livestock in Malaysia as revealed by amplification and sequencing of the internal transcribed spacer II DNA region. BMC Vet Res 2014; 10: 38. https://doi.org/10.1186/1746-6148-10-38
  14. Sato M, Yoonuan T, Sanguankiat S, Nuamtanong S, Pongvongsa T, Phimmayoi I, Phanhanan V, Boupha B, Moji K, Waikagul J. Short report: Human Trichostrongylus colubriformis infection in a rural village in Laos. Am J Trop Med Hyg 2011; 84: 52-54. https://doi.org/10.4269/ajtmh.2011.10-0385
  15. Gholami S, Babamahmoodi F, Abedian R, Sharif M, Shahbazi A, Pagheh A, Fakhar M. Trichostrongylus colubriformis: possible most common cause of human infection in Mazandaran province, North of Iran. Iran J Parasitol 2015; 10: 110-115.
  16. Ghadirian E, Arfaa F, Sadighian A. Human infection with Trichostrongylus capricola in Iran. Am J Trop Med Hyg 1974; 23: 1002-1003. https://doi.org/10.4269/ajtmh.1974.23.1002
  17. Ghadirian E. Human infection with Trichostrongylus lerouxi (Biocca, Chabaud, and Ghadirian, 1974) in Iran. Am J Trop Med Hyg 1977; 26: 1212-1213. https://doi.org/10.4269/ajtmh.1977.26.1212
  18. Shahbazi A, Fallah E, Koshki MHK, Nematollahi A, Chazanchaei A, Asfaram S. Morphological characterization of the Trichostrongylus species isolated from sheep in Tabriz, Iran. Res Opin Anim Vet Sci 2012; 2: 309-312.
  19. Ghasemikhah R, Mirhendi H, Kia E, Mowlavi G, Sarmadian H, Meshgi B, Golestan B, Mobedi I. Morphological and morphometrical description of Trichostrongylus species isolated from domestic ruminants in Khuzestan province, southwest Iran. Iran J Parasitol 2011; 6: 82-88.
  20. Mahjouri E. Historical Geography of Mazandaran Province, Tehran. Geographical Organization Press. 2001.
  21. Sharifdini M, Mirhendi H, Ashrafi K, Hosseini M, Mohebali M, Khodadadi H, Kia EB. Comparison of nested polymerase chain reaction and real-time polymerase chain reaction with parasitological methods for detection of Strongyloides stercoralis in human fecal samples. Am J Trop Med Hyg 2015; 93: 1285-1291. https://doi.org/10.4269/ajtmh.15-0309
  22. Repetto SA, Alba Soto CD, Cazorla SI, Tayeldin ML, Cuello S, Lasala MB, Tekiel VS, Gonzalez Cappa SM. An improved DNA isolation technique for PCR detection of Strongyloides stercoralis in stool samples. Acta Trop 2013; 126: 110-114. https://doi.org/10.1016/j.actatropica.2013.02.003
  23. Chilton NB. The use of nuclear ribosomal DNA markers for the identification of bursate nematodes (order Strongylida) and for the diagnosis of infections. Anim Health Res Rev 2004; 5: 173-187. https://doi.org/10.1079/AHR200497
  24. Borji H, Razmi GH, Movassaghi AR, Naghibi AG, Maleki M. A study on gastrointestinal helminths of camels in Mashhad abattoir, Iran. Iran J Vet Res 2010; 11: 174-179.
  25. Anvari-Tafti M, Sazmand A, Hekmatimoghaddam S, Moobedi I. Gastrointestinal helminths of camels (Camelus dromedarius) in center of Iran. Trop Biomed 2013; 30: 56-61.
  26. Biocca E, Chabaud A, Ghadirian E. Trichostrongylus lerouxi n. sp., parasite of Bos taurus. Parassitologia 1974; 16: 199-207.
  27. Rokni MB. The present status of human helminthic diseases in Iran. Ann Trop Med Parasitol 2008; 102: 283-295. https://doi.org/10.1179/136485908X300805
  28. Ashrafi K, Tahbaz A, Sharifdini M, Mas-Coma S. Familial Trichostrongylus infection misdiagnosed as acute fascioliasis. Emerg Infect Dis 2015; 21: 1869-1870. https://doi.org/10.3201/eid2110.141392
  29. Ahmadi M, Kia EB, Rezaeian M, Hosseini M, Kamranrashani B, Tarighi F. Prevalence of Strongyloides stercoralis and other intestinal parasites in rehabilitation centers in Mazandaran province, Northern Iran. J Mazandaran Univ Med Sci 2015; 25: 1-7 (in Persian).
  30. Kia EB, Mahmoudi M, Zahabiun F, Meamar AR. An evaluation on the efficacy of agar plate culture for detection of Strongyloides stercoralis. Iran J Parasitol 2007; 2: 29-34.
  31. Watthanakulpanich D, Pongvongsa T, Sanguankiat S, Nuamtanong S, Maipanich W, Yoonuan T, Phuphisut O, Boupha B, Moji K, Sato M, Waikagul J. Prevalence and clinical aspects of human Trichostrongylus colubriformis infection in Lao PDR. Acta Trop 2013; 126: 37-42. https://doi.org/10.1016/j.actatropica.2013.01.002
  32. Gasser RB, Hoste H. Genetic markers for closely-related parasiticnematodes. Mol Cell Probes 1995; 9: 315-320. https://doi.org/10.1016/S0890-8508(95)91588-5
  33. Yong TS, Lee JH, Sim S, Lee J, Min DY, Chai JY, Eom KS, Sohn WM, Lee SH, Rim HJ. Differential diagnosis of Trichostrongylus and hookworm eggs via PCR using ITS-1 sequence. Korean J Parasitol 2007; 45: 69-74. https://doi.org/10.3347/kjp.2007.45.1.69
  34. Cancrini G, Boemi G, Iori A, Corselli A. Human infestations by Trichostrongylus axei, T. capricola and T. vitrinus: 1st report in Italy. Parassitologia 1982; 24: 145-149 (in Italian).

피인용 문헌

  1. Gastrointestinal helminths in farmers and their ruminant livestock from the Coastal Savannah zone of Ghana vol.117, pp.10, 2017, https://doi.org/10.1007/s00436-018-6017-1
  2. Strongyloides stercoralis hyperinfection in an unconscious diabetic patient with dermatomyositis vol.61, pp.1, 2018, https://doi.org/10.4103/ijpm.ijpm_734_16
  3. Clinical and haematological characteristics of human trichostrongyliasis vol.93, pp.2, 2019, https://doi.org/10.1017/s0022149x17001225
  4. Zoonotic transmission of Teladorsagia circumcincta and Trichostrongylus species in Guilan province, northern Iran: molecular and morphological characterizations vol.20, pp.None, 2017, https://doi.org/10.1186/s12879-020-4762-0
  5. Strongyloides stercoralis and other intestinal parasites in patients receiving immunosuppressive drugs in northern Iran: a closer look at risk factors vol.43, pp.None, 2017, https://doi.org/10.4178/epih.e2021009
  6. Comparison of Molecular and Parasitological Methods for Diagnosis of Human Trichostrongylosis vol.11, pp.None, 2021, https://doi.org/10.3389/fcimb.2021.759396
  7. Trichostrongyloid nematodes in ruminants of northern Iran: prevalence and molecular analysis vol.17, pp.1, 2017, https://doi.org/10.1186/s12917-021-03086-3