생명과학회지 (Journal of Life Science)

  • 제27권6호
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  • Pages.611-616
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  • 2017
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
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한약재 추출물의 항균활성 검증

Verification of Antibacterial Activities of Oriental Herbal Medicine Extracts

  • 이창은 (신라대학교 일반대학원 그린화학융합공학과) ;
  • 조정권 (신라대학교 일반대학원 바이오과학과) ;
  • 김재덕 (신라대학교 일반대학원 그린화학융합공학과) ;
  • 이동근 (신라대학교 일반대학원 바이오과학과) ;
  • 김원석 (신라대학교 일반대학원 바이오과학과) ;
  • 이상현 (신라대학교 일반대학원 그린화학융합공학과)
  • Lee, Chang-Eun (Department of Green-Chemistry Convergence Engineering, Graduate School, Silla University) ;
  • Jo, Jung-Kwon (Department of Bioscience, Graduate School, Silla University) ;
  • Kim, Jae-Deok (Department of Green-Chemistry Convergence Engineering, Graduate School, Silla University) ;
  • Lee, Dong-Geun (Department of Bioscience, Graduate School, Silla University) ;
  • Kim, Won-Seok (Department of Bioscience, Graduate School, Silla University) ;
  • Lee, Sang-Hyeon (Department of Green-Chemistry Convergence Engineering, Graduate School, Silla University)
  • 투고 : 2016.12.13
  • 심사 : 2017.02.07
  • 발행 : 2017.06.30
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초록

식중독의 원인 세균 및 병원성 세균 7종(Bacillus cereus (11.2, 20), Clostridium perfringens (2.4, 4.7), Escherichia coli (9.1, 9.1), Listeria monocytogenes (11.1, 20), Salmonella typhi (4.7, 9.1), Staphylococcus aureus (2.4, 4.7), Vibrio parahaemolyticus (2.4, 5.3))에 대해 한약재 추출물의 항균 활성을 측정하였다. 추출물은 증류수를 이용한 고온추출법($121^{\circ}C$에서 30분간)으로 제조하였고, 9가지 한약재 추출물의 수율은 0.5-33.4% 범위였다. 종이 디스크 확산법을 이용하여 박테리아에 대한 항균 활성을 평가하였을 때 황련, 지유, 오배자 추출물에서만 항균활성이 나타났다. 황련과 지유 추출물은 Clostridium perfringens과 Listeria monocytogenes에만 항균활성을 나타낸 반면, 오배자는 시험한 모든 세균에 대해 강한 항균 작용을 보였다. 오배자 추출물의 건조시료가 나타내는 최소억제농도(MIC)와 최소살균농도(MBC)는 2.4~20 mg/ml 범위였다. 본 연구결과는 추후 정제와 항균물질에 대한 연구를 통하여 기존 항균제의 보완제 및 식품의 유통기한 증진 등 항균소재로서 응용이 가능할 것으로 기대된다.

The antibacterial activities of nine oriental herbal medicine extracts were measured against seven kinds of bacteria known to cause food poisoning and disease. The extracts were prepared with distilled water using an autoclave ($121^{\circ}C$ for 30 min). The extraction yields of nine oriental herbal medicines were in the rage of 0.5%-33.4%. The antibacterial activities were evaluated using the paper-disc method. Extracts of Hwangryeon, Jiyu, and Ohbaeja showed antibacterial activities while the others did not. Extracts of Coptis japonica, Hwangryeon, and Sanguisorba officinalis, Jiyu showed antibacterial activities only against Clostridium perfringens and Listeria monocytogenes. Extracts of Rhus javanica, Ohbaeja showed the strongest antibacterial activities against all the bacteria tested. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of R. javanica extracts were in the range of 2.4~20 mg/ml for the bacteria tested. In conclusion, the extract of R. javanica showed superior antibacterial activities against the seven bacterial strains tested. The MIC and MBC values for each bacterium were represented as strain names (MIC, MBC), which were Bacillus cereus (11.2, 20), Clostridium perfringens (2.4, 4.7), Escherichia coli (9.1, 9.1), Listeria monocytogenes (11.1, 20), Salmonella typhi (4.7, 9.1), Staphylococcus aureus (2.4, 4.7), and Vibrio parahaemolyticus (2.4, 5.3). Further purification and study of this antibacterial material would be helpful for developing antibiotics or promoting synergistic effects with known antibiotics, producing antibacterial material for lengthening the shelf-life of food, and so on.

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