한국수정란이식학회지 (Journal of Embryo Transfer)

  • 제32권2호
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  • Pages.39-45
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  • 2017
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  • 2508-755X(pISSN)
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  • 2288-0178(eISSN)
한국수정란이식학회 (The Korean Society of Embryo Transfer)
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An Increase in Mesenchymal Stem Cells Expressing Nestin in Bone-Marrow-Derived Primary Cells Stimulates Neurogenic Differentiation in Rat

  • Han, Na Rae (Department of Animal Life Science, Kangwon National University) ;
  • Lee, Hyun (Department of Animal Life Science, Kangwon National University) ;
  • Yun, Jung Im (Division of Animal Resource Science, Kangwon National University) ;
  • Kim, Choonghyo (Department of Neurosurgery, Kangwon National University Hospital, School of Medicine, Kangwon National University) ;
  • Hwang, Jae Yeon (Department of Cellular and Molecular Physiology, Yale School of Medicine) ;
  • Park, Kyu Hyun (Department of Animal Life Science, Kangwon National University) ;
  • Lee, Seung Tae (Department of Animal Life Science, Kangwon National University)
  • 투고 : 2017.02.10
  • 심사 : 2017.04.27
  • 발행 : 2017.06.30
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초록

Mesenchymal stem cells (MSCs) have been considered an alternative source of neuronal lineage cells, which are difficult to isolate from brain and expand in vitro. Previous studies have reported that MSCs expressing Nestin ($Nestin^+$ MSCs), a neuronal stem/progenitor cell marker, exhibit increased transcriptional levels of neural development-related genes, indicating that $Nestin^+$ MSCs may exert potential with neurogenic differentiation. Accordingly, we investigated the effects of the presence of $Nestin^+$ MSCs in bone-marrow-derived primary cells (BMPCs) on enhanced neurogenic differentiation of BMPCs by identifying the presence of $Nestin^+$ MSCs in uncultured and cultured BMPCs. The percentage of $Nestin^+$ MSCs in BMPCs was measured per passage by double staining with Nestin and CD90, an MSC marker. The efficiency of neurogenic differentiation was compared among passages, revealing the highest and lowest yields of $Nestin^+$ MSCs. The presence of $Nestin^+$ MSCs was identified in BMPCs before in vitro culture, and the highest and lowest percentages of $Nestin^+$ MSCs in BMPCs was observed at the third (P3) and fifth passages (P5). Moreover, significantly the higher efficiency of differentiation into neurons, oligodendrocyte precursor cells and astrocytes was detected in BMPCs at P3, compared with P5. In conclusion, these results demonstrate that neurogenic differentiation can be enhanced by increasing the proportion of $Nestin^+$ MSCs in cultured BMPCs.

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참고문헌

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