Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Antioxidant Effect and Component Analysis of Cardiospermum halicacabum Leaf Extracts

풍선덩굴 잎 추출물의 항산화 효과 및 성분 분석

  • Jeong, Hyo Jin (Department of Fine Chemistry, Cosmetic R&D center, Seoul National University of Science and Technology) ;
  • Kim, A Rang (Department of Fine Chemistry, Cosmetic R&D center, Seoul National University of Science and Technology) ;
  • Lee, Keon Soo (Department of Fine Chemistry, Cosmetic R&D center, Seoul National University of Science and Technology) ;
  • Park, So Hyun (Department of Fine Chemistry, Cosmetic R&D center, Seoul National University of Science and Technology) ;
  • Shin, Hyuk Soo (Department of Fine Chemistry, Cosmetic R&D center, Seoul National University of Science and Technology) ;
  • Lee, Sang Rae (Department of Fine Chemistry, Cosmetic R&D center, Seoul National University of Science and Technology) ;
  • Song, Ba Reum (Department of Fine Chemistry, Cosmetic R&D center, Seoul National University of Science and Technology) ;
  • Lee, Yun Ju (Department of Fine Chemistry, Cosmetic R&D center, Seoul National University of Science and Technology) ;
  • An, Hyun Jin (Yeomyung Biochem, Room 301, K hall, Startup Center, Chungcheong University) ;
  • Lee, Jae Duk (Yeomyung Biochem, Room 301, K hall, Startup Center, Chungcheong University) ;
  • Park, Soo Nam (Department of Fine Chemistry, Cosmetic R&D center, Seoul National University of Science and Technology)
  • 정효진 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소) ;
  • 김아랑 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소) ;
  • 이건수 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소) ;
  • 박소현 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소) ;
  • 신혁수 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소) ;
  • 이상래 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소) ;
  • 송바름 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소) ;
  • 이윤주 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소) ;
  • 안현진 (여명바이오켐) ;
  • 이재덕 (여명바이오켐) ;
  • 박수남 (서울과학기술대학교 정밀화학과, 화장품종합기술연구소)
  • Received : 2017.06.24
  • Accepted : 2017.06.30
  • Published : 2017.06.30
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Abstract

In this study, the antioxidant effect and component analysis for extract and fractions of Cardiospermum halicacabum leaf were investigated. All experiments were performed with 50% ethanol extract, ethyl acetate fraction and aglycone fraction obtained from dried C. halicacabum leaf. The yields of extract and fractions were 16.4, 0.9 and 0.3% per dried powder, respectively. DPPH (1,1-phenyl-2-picrylhydrazyl) radical scavenging activity ($FSC_{50}$) of ethyl acetate fraction ($92.5{\mu}g/mL$) was the greatest radical scavenging activity, but lower than (+)-${\alpha}$-tocopherol ($8.9{\mu}g/mL$). In reactive oxygen species (ROS) scavenging activity (total antioxidant capacity, $OSC_{50}$) on ROS generated in $Fe^{3+}-EDTA/H_2O_2$ system, aglycone fraction ($4.2{\mu}g/mL$) was the highest total antioxidant capacity and similar to L-ascorbic acid ($1.5{\mu}g/mL$). The cellular protective effects of C. halicacabum leaf extract and fractions on the $^1O_2$-induced cellular damage of human erythrocytes were exhibited at all concentration-dependent ($5.0-25.0{\mu}g/mL$). Especially, aglycone fraction (${\tau}_{50}$, 76.4 min) in $25.0{\mu}g/mL$ showed the most protective effect among extracts. Components of the ethyl acetate fraction obtained from C. halicacabum extracts were analyzed by TLC, HPLC chromatogram and LC/ESI-MS. Results showed that the ethyl acetate fraction contained some flavonoids, such as apigenin-7-O-glucuronide, apigenin-7-glucosdie and quercitrin hydrate. These results suggest that the extracts and fractions of C. halicacabum leaf may be applied as antioxidant functional cosmetic raw materials.

본 연구에서는 풍선덩굴 잎 추출물 및 그 분획물에 대하여 항산화 활성 평가와 성분 분석을 수행하였다. 본 실험에는 풍선덩굴 건조 잎의 50% 에탄올 추출물, 에틸아세테이트 분획 및 아글리콘 분획을 사용하였으며, 각각의 수율은 16.4, 0.9 및 0.3%로 나타났다. 자유라디칼 소거활성(1,1-diphenyl-2-picrylhydrazyl, DPPH, $FSC_{50}$)은 에틸아세테이트 분획($92.5{\mu}g/mL$)이 가장 큰 것으로 나타났으며, 이때 대조군인 (+)-${\alpha}$-tocopherol의 $FSC_5$은 $8.9{\mu}g/mL$이었다. $Fe^{3+}-EDTA/H_2O_2$계를 이용한 활성산소 소거활성(총항산화능, $OSC_{50}$)은 아글리콘 분획($4.2{\mu}g/mL$)에서 가장 크게 나타났으며, 대조군인 L-ascorbic acid ($1.5{\mu}g/mL$)와 유사한 효과를 나타내었다. $^1O_2$로 유도된 사람 세포 손상에 대한 보호효과 측정에서 풍선덩굴 잎 추출물 및 분획은 모두 농도 의존적($5.0-25.0{\mu}g/mL$)으로 세포보호효과를 나타냈다. 추출물/분획물 중에서 아글리콘 분획(${\tau}_{50}$, 76.4 min)이 가장 큰 세포보호효과를 나타내었다. TLC, HPLC, LC/ESI-MS를 이용하여 풍선덩굴 잎 추출물 중 에틸아세테이트 분획물에 대하여 성분 분석을 실시하였다. 그 결과, apigenin-7-O-glucuronide, apigenin-7-glucosdie 및 quercitrin hydrate 등의 플라보노이드가 함유되어 있음을 확인하였다. 이상의 결과들은 풍선덩굴 잎 추출물 또는 분획이 항산화 기능성 화장품 원료로서 응용 가능성이 있음을 시사한다.

Keywords

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