Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Effects of Probiotic Microbes on Growth Performance, Innate Immunity, and Pathogen Sensitivity in Cultured Olive Flounder

Probiotic 기능을 가진 미생물을 함유한 양어용 생균제가 넙치의 성장, 선천성면역 및 항병능에 미치는 영향

  • Lee, Ji-Hoon (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Chae, Young-Sik (SGS Korea, Knowledge Industrial Center) ;
  • Park, Jung-Jin (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Choi, Jun-Ho (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Kim, Dong-Gun (R & D Center, Cheil-Bio Co.) ;
  • Park, Kwan Ha (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
  • 이지훈 (군산대학교 수산생명의학과) ;
  • 채영식 (한국에스지에스) ;
  • 박정진 (군산대학교 수산생명의학과) ;
  • 최준호 (군산대학교 수산생명의학과) ;
  • 김동건 (제일바이오 연구소) ;
  • 박관하 (군산대학교 수산생명의학과)
  • Received : 2017.05.15
  • Accepted : 2017.05.25
  • Published : 2017.06.30
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Abstract

Probiotic principles can be applied in aquaculture for the purpose of growth and immunity stimulation, disease prevention and eventually better production performance. This study was to assess effects of combinations of microbes containing two Bacillus sp., plus one Lactobacillus sp. as the basal preparation (BSL-LAB), and additional Nitrosomonas sp. (nitrifying bacteria consortium, NBS) in olive flounder (Paralichthys olivaceus). The effects examined were growth parameters, hematologic parameters, innate immunity and pathogen challenge test. Fish were assigned to 4 treatments as Control (no probiotics), Group A (Bacillius and Lactobacillus to culture water), Group B (Bacillius and Lactobacillus both in water and feed), Group C (same as Group B with additional Nitrosomonas in feed). Fish were allocated to the above 4 groups, each group being composed of triplicate 30 fish, for a 7-week feeding in the laboratory. Positive effects were observed both in growth and pathogen sensitivity with all three probiotic combinations. Such effects were attributed to improved innate immune functions. This result indicates that the tested probiotic microbes are beneficial to olive flounder aquaculture.

생균제는 성장촉진, 면역증진 및 질병예방에서 넙치 Paralichthys olivaceus 치어의 양식에서 우수한 생산효과로 적용될 수 있다. 성장률, 선천성면역 및 항병력을 증강시킬 수 있는 생균제의 효과를 조사하기위해 Bacillus subtilis, Bacillus licheniformis 및 Lactobacillus plantarum (BSL-LAB)이 혼합되어있는 생균제와 NBS (nitrifying bacteria con- sortium) 분말을 사료에 첨가 또는 수조에 투입하여 시험을 수행하였다. 생균제 첨가사료는 7주간 투여하였고 3가지의 생균제 조합 시험군 모두에서 넙치의 성장과 항병력에서 대조군에 비해 높은 유효성을 발휘 하였다. 어병세균에 대한 항병력의 긍정적인 결과는 생균제에 대한 면역기능의 향상으로 나타난 효과이며 상기 시험결과로 볼 때, 넙치치어 양식에서 생균제의 혼합첨가는 성장 및 선천성면역 뿐만 아니라 질병저항성에 좋은 효과를 나타낼 수 있을 것으로 사료된다.

Keywords

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