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Antioxidation activity of residue after omija (Schisandra chinensis) juice extract

오미자(Schisandra chinensis) 착즙박 추출물의 항산화 활성

  • Park, Bo Na (Department of Food & Biotechnology and Biotechnology Graduate School, Hankyong National University) ;
  • Lee, Jin-Won (Faculty of Health & Food/Medicinal Plant, Suwon Women's University)
  • Received : 2016.12.28
  • Accepted : 2017.02.04
  • Published : 2017.06.30

Abstract

The physicochemical properties and schizandrin contents of various solvent ($H_2O$, 50% EtOH, 75% EtOH, 95% EtOH) extracts from residue after Omija juice was investigated using total polyphenol contents (TOC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity (RAS), anthocyanin contents (ANC), and schizandrin contents level (SCL). Total polyphenol contents, radical scavenging activity, and anthocyanin contents of 50% EtOH extract were the highest among all residue after Omija juice extracts, and was 16.70 mg/mL in the TOC and 86.16% in the DPPH-RAS. This meant that 50% EtOH extract from residue after Omija juice had more available antioxidant matters. As extraction time increases all extract treatments significantly reduced in the ANC contents (p <0.05). Amount of the SCL were observed higher value in 95% EtOH extract of residue after Omija juice.

오미자를 착즙하고 남은 착즙박을 이용하여 추출조건에 따른 이화학적 변화를 알아보았다. 즉, 착즙박을 열수, 50% 에탄올, 70% 에탄올 및 95% 에탄올을 추출 용매로 사용하였으며, 추출시간은 1시간에서 10시간까지 1시간 간격으로 $60^{\circ}C$에서 정치 추출하여 여과한 후, 감압 농축하여 제조된 용매별 농축액을 분석시료로 이용하였다. 오미자에 함유된 유효성분 중 하나인 schizandrin 함량은 추출 용매에 따라서 열수: 0.52 g/100 g, 50% 에탄올: 1.79 g/100 g, 70% 에탄올: 2.01 g/100 g 및 95% 에탄올은 2.37 g/100을 나타내었으며, 열수를 이용하여 추출한 경우보다 에탄올 농도가 증가할수록 schizandrin 함량이 증가하는 경향을 나타내었다. 총 폴리페놀 함량은 50% 에탄올 용매 추출물에서 16.70 mg/mL로 가장 높게 나타났으며, 2,2-diphenyl-1-picrylhydrazyl 전자공여소거능에 대한 효과는 분석 시료의 200 mg/mL 농도에서 측정하였을 때, 50% 에탄올 용매 추출물에서 86.16%로 가장 높게 나타내었다. 또한, 안토시아닌 함량은 모든 용매 추출물이 추출 시간이 증가할수록 감소하는 경향을 보였다.

Keywords

References

  1. Blois MS (1958) Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200 https://doi.org/10.1038/1811199a0
  2. Choi EO, Lee BS, Park YS, Seo EO, Chung BW (2008) Extraction condition from schizandra chinensis baillon for beverage development of high scizandrin concentration. Korean Soc J Biotechnol Bioeng 23: 271-275
  3. Choi HJ, Choi YW, Baek SY, Kim BS, Ahn SC, Rhee MS, Yoon S (2013) Activity-guided screening of anti-inflammatory compounds from the hexane extracts of schisandra chinensis fruit. J Life Science 23: 311-318 https://doi.org/10.5352/JLS.2013.23.2.311
  4. Choi SR, Kim CS, Kim JY, You DH, Kim JM, Kim YS, Song EJ, Kim YG, Ahn YS, Choi DG (2011) Changes of antioxidant activity and lignan contents in schisandra chinensis by harvesting times. Korean J Medicinal Crop Sci 19: 414-420 https://doi.org/10.7783/KJMCS.2011.19.6.414
  5. Chun HS, Kim HJ, Cho SB (2003) Effects of selected stabilizers on the color deterioration of crude pigrnent extract from schizandra fruit (Schizandra Fructus). Korean J Food Culture 18: 475-482
  6. Jeong PH, Kim YS, Shin DH (2006) Changes of physicochemical characteristics of schizandra chinensis during postharvest ripening at various temperatures. Koeran J Food Sci Technol 38: 469-474
  7. Jung GT, Ju IO, Choi JS, Hong JS (2000) The antioxudative, antimicrobial and nitrite scavenging effects of schizandra chinensis ruprecht (Omija) seed. Korean J Food Sci Technol 32: 928-935
  8. Kim HS (2013) Comparison of the content of physiological active substances in schisandra fruit tea and ginger tea by extraction method. Dissertation, Dankook University
  9. Kim KS, Kang SS, Ryu SN (2002) Quantitative analysis of lignans from fruits of schizandra chinensis. Kor J Pharmacogn 33: 272-276
  10. Kim KS, Park CG, Ryu SN, Bang JK, Lee BH (2000) Schizandrin, oil compounds, and their extraction yield in fruits of schizandra chinensis baillon. Korean J Crop Sci 45: 158-162
  11. Kim MJ, Park EJ (2010) Antioxidative and antigenotoxic effect of Omija (Schizandra chinensis B.) extracted with various solvents. J Korean Soc Food Sci Nutr 39: 487-493 https://doi.org/10.3746/jkfn.2010.39.4.487
  12. Kim SI, Sim KH, Ju SY, Han YS (2009) A Study of antioxidative and hypoglycemic activities of Omija (Schizandra chinensis baillon) extract under variable extract conditions. Korean J Food & Nutr 22: 41-47
  13. Kim YJ, Ha N, Han SH, Jeon JY, Hwang MH, Im YJ, Lee SY, Chae SW, Kim MG (2013) Confirmation of schizandrin as a marker compound in jangsu omija powder. J Korean Soc Food Sci Nutr 42: 244-248 https://doi.org/10.3746/jkfn.2013.42.2.244
  14. Kim YJ, Han SH, Jeon JY, Hwang MH, Im YJ, Chae SW, Kim MG (2012) Method development of ellagic acid as marker compound for standardization of gochang bokbunja (Rubus coreanus Miquel) as functional ingredient. J. Korean Soc Food Sci Nutr 41: 1554-1558 https://doi.org/10.3746/jkfn.2012.41.11.1554
  15. Ko BS, Park SK, Choi SB, Jun DW, Choi MK, Park SM (2004) A study on hypoglycemic effects of crude extracts of schizandrae fructus. J Korean Soc Appl Biol Chem 47: 258-264
  16. Kwon HJ, Park CS (2006) Biological activities of extracts from Omija (Schizandra chinensis Baillon). Korean J Food Preserv 15: 587-592
  17. Kwon J, Lee SJ, So JN, Oh CH (2001) Effects of schizandra chinensis fructus on the immunoregulatory action and apoptosis of L1210 cells. Korean J Food Sci Technol 33: 384-388
  18. Lee JS, Lee SW (1990) Effects of water extracts in fruits of Omija (Schizandra chinensis baillon) on alcohol metabolism. J Korean Soc Food Culture 5: 259-263
  19. Mok CY (2005) Quality characteristics of instant tea prepared from spraydried Omija (Schizandra chinensis Baillon) extract/grape juice mixture. Food Eng Prog 9: 226-230
  20. Moon YJ, Park S, Sung CK (2003) Effect of ethanolic extract of schizandra chinensis for the delayed ripening Kimchi preparation. Food Sci Nutr 16: 7-14
  21. Ock ES (1995) Effect of schizandra chinensis extract in hyperlipidemic rats. J Korean Soc Food Nutr 24: 658-662
  22. Park EJ, Ahn JJ, Kwon JH (2013) Effect of reflux conditions on extraction properties and antioxidant activity of freeze dried-schisandra chinensis. Korean J Food Sci. Technol 45: 550-556 https://doi.org/10.9721/KJFST.2013.45.5.550
  23. Park HM, Hong JH (2014) Physiological activities of aronia melanocarpa extracts on extraction solvents. Korean J Food Preserv 21: 718-726 https://doi.org/10.11002/kjfp.2014.21.5.718
  24. Rho SN, Oh HS (2002) Effect of Omija (Schizandra chinensis baillon) extracts on the growth of liver cancer cell line SNU-398. Korean J Nutr 35: 201-206
  25. Ryu MJ, Chung HS (2011) Effect on hot water extract of schizandra chinensis on colon cancer cell line. Food Eng Prog 15: 64-69
  26. SAS Institute (1988) SAS/STAT User Guide, release 6.30 edition. Washington, D.C, USA
  27. Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Amer J Enol Viticult 16: 144-158
  28. Sohn HJ, Bock JY (1989) Identification of lignan compounds in fruits of schisandra chinensis baillon by gas chromatography/mass spectrometry. J Korean Agric Chem Soc 32: 344-349
  29. Song GB (1982) Anthocyanin in cultured omija (schizandra chinensis baillon) and its stability. Dissertation, Chonbuk National University
  30. Whang IT (2002) Study on the effects of fructus schizandrae on the cerebral blood flow and blood pressure. Dissertation, Wonkwang University
  31. Yukinobu I, Heihachiro T, Itiro Y, Hiroshi K (1979) The constituents of schizandra chinensis baill. I. isolation and structure determination of five new lignans, gomisin A, B, C, F and G, and the absolute structure of schizandrin. Chem Pharm Bull 27: 1383-1394 https://doi.org/10.1248/cpb.27.1383

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