Ocean and Polar Research

Korea Institute of Ocean Science & Technology (한국해양과학기술원)
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RNA-Seq Transcriptome Analysis of the Cutlass Fish Reveals Photoreceptors Gene Expression in Peripheral Tissues

RNA-Seq transcriptome 분석을 통한 갈치 광수용체 유전자 탐색 및 mRNA 조직발현

  • Hyeon, Ji-Yeon (Jeju International Marine Science Research & Logistics Center, KIOST) ;
  • Kim, Mun-Kwan (Jeju Special Self-Governing Province Oceans and Fisheries Research Institute) ;
  • Lim, Bong-Soo (Solforto Co. Ltd.) ;
  • Byun, Jun-Hwan (Jeju International Marine Science Research & Logistics Center, KIOST) ;
  • Moon, Ji-Sung (Jeju International Marine Science Research & Logistics Center, KIOST) ;
  • Kang, Hyeong-Cheol (Jeju Special Self-Governing Province Oceans and Fisheries Research Institute) ;
  • Hur, Sung-Pyo (Jeju International Marine Science Research & Logistics Center, KIOST) ;
  • Oh, Seong-Rip (Jeju Special Self-Governing Province Oceans and Fisheries Research Institute)
  • 현지연 (한국해양과학기술원 제주국제해양과학연구지원센터) ;
  • 김문관 (제주특별자치도 해양수산연구원) ;
  • 임봉수 ((주)솔포투) ;
  • 변준환 (한국해양과학기술원 제주국제해양과학연구지원센터) ;
  • 문지성 (한국해양과학기술원 제주국제해양과학연구지원센터) ;
  • 강형철 (제주특별자치도 해양수산연구원) ;
  • 허성표 (한국해양과학기술원 제주국제해양과학연구지원센터) ;
  • 오성립 (제주특별자치도 해양수산연구원)
  • Received : 2017.04.17
  • Accepted : 2017.05.26
  • Published : 2017.06.30
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Abstract

The opsin family of light sensitive proteins family makes up are the universal photoreceptor molecules of all visual systems in the vertebrates including teleosts. They can change their conformation from a resting state to a signaling state upon light absorption, which activates the G-protein coupled receptor, thereby resulting in a signaling cascade that produces physiological responses. However, this species is poorly characterized at molecular level due to little sequence information available in public databases. We have investigated the opsin family of nocturnal cutlass fish using the whole transcriptome sequencing method. The opsin genes were cloned and its expression in the tissues and organs were examined by qPCR. We cloned 6 opsin genes (RRH, Opn4, Rh1, Rh2, VA-opsin, and Opn3) in retina and brain tissue. It contained the seven presumed transmembrane domains that are characteristic of the G-protein-coupled receptor family. However, short wavelength sensitive pigment (SWS) and long wavelength sensitive pigment (LWS) were not detected in this study. The mRNA expression of the 6 photoreceptor genes were detected in retina and peripheral tissue. Our studies will lead to further investigation of the photic entrainment mechanism at molecular and cellular levels in cutlass fish and can be used in comparative studies of other fishes.

Keywords

References

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